中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2005年
1期
196-197
,共2页
安玉会%蔡尚党%崔福爱%赵荷键
安玉會%蔡尚黨%崔福愛%趙荷鍵
안옥회%채상당%최복애%조하건
痴呆,血管性%疾病模型,动物%一氧化氮
癡呆,血管性%疾病模型,動物%一氧化氮
치태,혈관성%질병모형,동물%일양화담
背景:应用酸性肽对血管型痴呆(vascular dementia,VD)小鼠模型进行治疗研究,是否可以发现酸性肽的治疗效果.目的:研究酸性肽对血管型模型痴呆小鼠学习记忆能力和它们脑中蛋白质和一氧化氮含量的变化并与正常小鼠相比较.设计:随机对照的实验研究.单位:郑州大学医学院生物化学与分子生物学教研室.对象:实验于2002-10/2003-02在郑州大学医学院生物化学与分子生物学教研室的第一研究室和动物房进行.取昆明种小鼠84只,跳台实验低于正常小鼠平均反应时间的1只动物被排除.随机分为7组:正常组,模型组,盐水组,脑复康组,酸生肽高浓度组,酸性肽中浓度组,酸性肽低浓度组.方法:除12只正常组小鼠外,其余的动物一直用高脂乳剂0 5 mL/d灌胃共10 d,按照高维娟等的方法建立VD动物模型.模型组不作任何治疗,盐水组用生理盐,脑复康组应用脑复康,酸性肽高、中、低组应用不同浓度酸性肽治疗,口服给药治疗15 d.之后用跳台试验进行学习记忆功能的变化测试,随后处死小鼠,测定脑中蛋白质和一氧化氮水平.主要观察指标:各组小鼠学习记忆功能和脑中蛋白质和一氧化氮水平.结果:①跳台试验的结果表明酸性肽能明显地减少痴呆小鼠跳台试验的错误次数,能显著地增加痴呆小鼠的学习记忆能力.②生化分析结果表明,模型组小鼠因脑缺血而使脑中蛋白质的含量[3.530 9±0.327 8)g/L]较正常组[4.028 3±0.378 3)g/L]减少.用酸性肽治疗痴呆小鼠后,其脑中的蛋白质含量[高、中、低浓度组分别为:(4.908 3±0.515 3),(4.752 5±0 471 7),(4.376 7±0.370 7)g/L]比模型组和生理盐水治疗组均显著增加.说明酸性肽能促进脑内蛋白合成.这有利于受损脑细胞的修复和功能恢复.③酸性肽可显著地抑制NO的合成.这将会减少一氧化氮对脑细胞的毒性作用.结论:酸性肽能显著增强VD小鼠的学习记忆能力,有利于受损脑细胞的修复和功能恢复.
揹景:應用痠性肽對血管型癡呆(vascular dementia,VD)小鼠模型進行治療研究,是否可以髮現痠性肽的治療效果.目的:研究痠性肽對血管型模型癡呆小鼠學習記憶能力和它們腦中蛋白質和一氧化氮含量的變化併與正常小鼠相比較.設計:隨機對照的實驗研究.單位:鄭州大學醫學院生物化學與分子生物學教研室.對象:實驗于2002-10/2003-02在鄭州大學醫學院生物化學與分子生物學教研室的第一研究室和動物房進行.取昆明種小鼠84隻,跳檯實驗低于正常小鼠平均反應時間的1隻動物被排除.隨機分為7組:正常組,模型組,鹽水組,腦複康組,痠生肽高濃度組,痠性肽中濃度組,痠性肽低濃度組.方法:除12隻正常組小鼠外,其餘的動物一直用高脂乳劑0 5 mL/d灌胃共10 d,按照高維娟等的方法建立VD動物模型.模型組不作任何治療,鹽水組用生理鹽,腦複康組應用腦複康,痠性肽高、中、低組應用不同濃度痠性肽治療,口服給藥治療15 d.之後用跳檯試驗進行學習記憶功能的變化測試,隨後處死小鼠,測定腦中蛋白質和一氧化氮水平.主要觀察指標:各組小鼠學習記憶功能和腦中蛋白質和一氧化氮水平.結果:①跳檯試驗的結果錶明痠性肽能明顯地減少癡呆小鼠跳檯試驗的錯誤次數,能顯著地增加癡呆小鼠的學習記憶能力.②生化分析結果錶明,模型組小鼠因腦缺血而使腦中蛋白質的含量[3.530 9±0.327 8)g/L]較正常組[4.028 3±0.378 3)g/L]減少.用痠性肽治療癡呆小鼠後,其腦中的蛋白質含量[高、中、低濃度組分彆為:(4.908 3±0.515 3),(4.752 5±0 471 7),(4.376 7±0.370 7)g/L]比模型組和生理鹽水治療組均顯著增加.說明痠性肽能促進腦內蛋白閤成.這有利于受損腦細胞的脩複和功能恢複.③痠性肽可顯著地抑製NO的閤成.這將會減少一氧化氮對腦細胞的毒性作用.結論:痠性肽能顯著增彊VD小鼠的學習記憶能力,有利于受損腦細胞的脩複和功能恢複.
배경:응용산성태대혈관형치태(vascular dementia,VD)소서모형진행치료연구,시부가이발현산성태적치료효과.목적:연구산성태대혈관형모형치태소서학습기억능력화타문뇌중단백질화일양화담함량적변화병여정상소서상비교.설계:수궤대조적실험연구.단위:정주대학의학원생물화학여분자생물학교연실.대상:실험우2002-10/2003-02재정주대학의학원생물화학여분자생물학교연실적제일연구실화동물방진행.취곤명충소서84지,도태실험저우정상소서평균반응시간적1지동물피배제.수궤분위7조:정상조,모형조,염수조,뇌복강조,산생태고농도조,산성태중농도조,산성태저농도조.방법:제12지정상조소서외,기여적동물일직용고지유제0 5 mL/d관위공10 d,안조고유연등적방법건립VD동물모형.모형조불작임하치료,염수조용생리염,뇌복강조응용뇌복강,산성태고、중、저조응용불동농도산성태치료,구복급약치료15 d.지후용도태시험진행학습기억공능적변화측시,수후처사소서,측정뇌중단백질화일양화담수평.주요관찰지표:각조소서학습기억공능화뇌중단백질화일양화담수평.결과:①도태시험적결과표명산성태능명현지감소치태소서도태시험적착오차수,능현저지증가치태소서적학습기억능력.②생화분석결과표명,모형조소서인뇌결혈이사뇌중단백질적함량[3.530 9±0.327 8)g/L]교정상조[4.028 3±0.378 3)g/L]감소.용산성태치료치태소서후,기뇌중적단백질함량[고、중、저농도조분별위:(4.908 3±0.515 3),(4.752 5±0 471 7),(4.376 7±0.370 7)g/L]비모형조화생리염수치료조균현저증가.설명산성태능촉진뇌내단백합성.저유리우수손뇌세포적수복화공능회복.③산성태가현저지억제NO적합성.저장회감소일양화담대뇌세포적독성작용.결론:산성태능현저증강VD소서적학습기억능력,유리우수손뇌세포적수복화공능회복.
BACKGROUND: If it can prove the therapeutic effects of acidic peptide when applying it to the mouse model of vascular dementia(VD).OBJECTIVE: To study the memory ability, change of contents of protein and nitric oxide of mouse model of VD and make comparison with normal mice.DESIGN: A randomized and experimental study.SETTING: Biochemistry & Molecular Biology Teaching Section of College of Medicine, Zhengzhou University.PARTICIPANTS: The experiment was completed in the First Research Unit and Animal Centre of Biochemistry & Molecular Biology Teaching Section of Zhengzhou University. Totally 84 mice of Kunming species were selected and the mouse was excluded because its average response time was slower than normal mouse when performing step-down avoidance test. The mice were randomly divided into 7 groups: normal group, model group, saline group,naofukang group, high dose acidic peptide group, moderate dose acidic peptide group and low dose acidic peptide group.METHODS: All the mice excepts of 12 in normal group were conducted stomach perfusion of high fat emulsion 0.5 mL per day for 10 days to establish VD animal model according to the method of Gao et al. There was no treatment for model group. While saline, acidic peptide high, moderate and low dose of acidic peptide was orally administrated to saline group, naofukang group, high dose, moderate dose and low dose acidic peptide group respectively for 15days. Then learning and memory functions were tested by step-down avoidance test. After being executed, protein and nitric oxide in mouse brain was assayed.MAIN OUTCOME MEASURES: Learning and memory ability of mice in each group as well as the content of protein and nitric oxide in brain.peptide had greatly reduced the mistaken times in mice and dramatically chemical analysis showed that the content of protein in mice of model group [ (3. 530 9 ± 0. 327 8) g/L] was reduced comparing with mice in normal group [ (4. 028 3 ± 0. 378 3) g/L] . After administrating acidic peptide to dementia mice, the protein content in high, moderate and low dose group was (4. 908 3 ± 0. 515 3) g/L, (4. 752 5 ± 0. 471 7) g/L and(4. 376 7 ±0. 370 7) g/L respectively which was greatly increased when compared with model group and saline group. It suggested that acidic peptide could stimulate the synthesis of protein in brain, which was good for the resynthesis of nitric oxide and reduced the toxic effects of NO to brain cells.CONCLUSION: Acidic peptide can improve the ability of learning and memory of mice with VD greatly and also has good effects on the plerosis of injured cerebral cells and the rehabilitation of its function.
