CAJ | 학술논문

目的探讨电针对抑郁症大鼠的治疗作用及海马cAMP反应元件结合蛋白(CREB)、脑源性神经营养因子(BDNF)的影响.方法 SD成年大鼠32只随机分为空白组、模型组、针刺组(合谷+太冲)和药物组(盐酸氟西汀),每组8只.空白组进行正常饲养,不给予其他处理;模型组大鼠采用长期不可预见性温和刺激造成抑郁症模型;电针组大鼠造模后电针合谷穴和太冲穴,每日1次,每次15 min,左右两侧穴位交替进行,持续21 d.药物组大鼠造模后进行氟西汀灌胃,每日1次,持续3 w.应激后第7、14、22天观察大鼠Open field行为学的变化,应激后22 d取材观察海马神经元形态结构、BDNF和CREB的表达.结果 (1)应激后第7、14、22天模型组大鼠水平运动及垂直运动较应激前有不同程度的减弱,针刺组于应激后第14天可有效地改善抑郁型大鼠模型的行为学表现,其效果优于药物组(P<0.05).(2)模型组海马齿状回神经元排列散乱,可见明显肿胀、固缩、变性、脱落等病理改变;针刺组和药物组大鼠海马神经元的病变程度较模型组有不同程度的减轻.(3)模型组大鼠BDNF阳性细胞数量较空白组明显减少(P<0.05),针刺组和药物组海马BDNF和CREB的表达较模型组上调(P<0.05).结论针刺能改善抑郁症大鼠的行为学及海马神经元的病理变化,CREB-BDNF受体后信号转导通路是针刺抗抑郁的重要途径和作用靶点之一.
목적 탐토전침대억욱증대서적치료작용급해마cAMP반응원건결합단백(CREB)、뇌원성신경영양인자(BDNF)적영향.방법 SD성년대서32지수궤분위공백조、모형조、침자조(합곡+태충)화약물조(염산불서정),매조8지.공백조진행정상사양,불급여기타처리;모형조대서채용장기불가예견성온화자격조성억욱증모형;전침조대서조모후전침합곡혈화태충혈,매일1차,매차15 min,좌우량측혈위교체진행,지속21 d.약물조대서조모후진행불서정관위,매일1차,지속3 w.응격후제7、14、22천관찰대서Open field행위학적변화,응격후22 d취재관찰해마신경원형태결구、BDNF화CREB적표체.결과 (1)응격후제7、14、22천모형조대서수평운동급수직운동교응격전유불동정도적감약,침자조우응격후제14천가유효지개선억욱형대서모형적행위학표현,기효과우우약물조(P<0.05).(2)모형조해마치상회신경원배렬산란,가견명현종창、고축、변성、탈락등병리개변;침자조화약물조대서해마신경원적병변정도교모형조유불동정도적감경.(3)모형조대서BDNF양성세포수량교공백조명현감소(P<0.05),침자조화약물조해마BDNF화CREB적표체교모형조상조(P<0.05).결론 침자능개선억욱증대서적행위학급해마신경원적병리변화,CREB-BDNF수체후신호전도통로시침자항억욱적중요도경화작용파점지일.
Objective To explore the therapeutic effect of electroacupuncture on depresssion rats and cyclic adenosine monophosphate (cAMP) response element binding protein (CREB) and brain-derived neurotrophic factor (BDNF). Methods Thirty-two adult Sprague-Dawley rats were divided randomly into control, model, electroacupuncture (EA) and medicine groups, 8 rats in each group. Rats in control group did not receive any other stimulation, and rats in model group were made depression by the chronic mild stress. In EA group, rats were given EA stimulation for 15 minutes, once per day for 3 w, following stress stimulation. Rats in medicine group were treated with fluoxetine hydrochloride for 3 w. Changes of open-field behavior of the rats were observed in the 7th, 14th and 22th d following stress. Morphological changes of hippocampus neurons and the expression of CREB and BDNF were measured in the 22th d. Results (1)The open field behavior of the depression rats decreased significantly following stress, and EA treatment effectively improved it, which was better than that of the antidepressant(P<0.05).(2) In the depression rats, neurons in the hippocampus were dispersedly distributed in the dentate gyrus ( DG ), which showed edema, atrophy and denaturation. Both EA and antidepressant treatment improved the neuronal damage in the DG. (3) The number of BDNF positive neurons in model group were remarkably decreased compared with that of control group. In EA group and medicine group, both expressions of CREB and BDNF in the hippocampus were up-regulated obviously(P＜0.05).Conclusions Acupuncture could improve significantly the changes of the behavior, pathological changes of neurons in the hippocampus, and CREB-BDNF postreceptor signal transduction pathway may be an important approach and target of acupuncture in treating depression.