CAJ | 학술논문

目的:观察肾衰2号方对慢性肾衰(chronic renal failure,CRF)大鼠肾皮质环氧化酶2(cyclooxygenase-2,COX-2)及环氧化酶1(cyclooxygenase-1,COX-1)mRNA表达的影响.方法:采用左肾动脉的2/3分支结扎,右肾摘除(ablation/infarction, A/I)法制作大鼠CRF模型.造模成功后,将造模大鼠随机分为模型组、西乐葆(塞来昔布)组、肾衰2号方组.选用10只正常大鼠作为正常对照组.治疗2个月后,苏木精和伊红染色观察大鼠肾组织形态学改变,检测治疗前后大鼠血清肌酐(serum creatinine,SCr)、尿素氮(blood urea nitrogen,BUN)水平,并用逆转录实时聚合酶链反应检测肾组织中COX-2和COX-1 mRNA的表达.结果:肾衰2号方能明显降低CRF大鼠SCr和BUN水平,改善肾功能及肾组织形态,并能明显降低CRF大鼠肾皮质COX-2 mRNA表达量,但对COX-1 mRNA表达无明显影响.结论:肾衰2号方可通过抑制COX-2 mRNA的过度表达改善肾功能,减轻肾小球硬化和纤维化.
목적:관찰신쇠2호방대만성신쇠(chronic renal failure,CRF)대서신피질배양화매2(cyclooxygenase-2,COX-2)급배양화매1(cyclooxygenase-1,COX-1)mRNA표체적영향.방법:채용좌신동맥적2/3분지결찰,우신적제(ablation/infarction, A/I)법제작대서CRF모형.조모성공후,장조모대서수궤분위모형조、서악보(새래석포)조、신쇠2호방조.선용10지정상대서작위정상대조조.치료2개월후,소목정화이홍염색관찰대서신조직형태학개변,검측치료전후대서혈청기항(serum creatinine,SCr)、뇨소담(blood urea nitrogen,BUN)수평,병용역전록실시취합매련반응검측신조직중COX-2화COX-1 mRNA적표체.결과:신쇠2호방능명현강저CRF대서SCr화BUN수평,개선신공능급신조직형태,병능명현강저CRF대서신피질COX-2 mRNA표체량,단대COX-1 mRNA표체무명현영향.결론:신쇠2호방가통과억제COX-2 mRNA적과도표체개선신공능,감경신소구경화화섬유화.
Objective: To observe the effects of No.2 Renal Failure Recipe (No.2RFR), a compound traditional Chinese herbal medicine, on expressions of cyclooxygenase-2 (COX-2) and cyclooxygenase-1 (COX-1) mRNAs in rats with chronic renal failure (CRF).Methods: A rat model of CRF was successfully established by infarction of approximately two-thirds of the left kidney and removal of the right kidney (ablation/infarction, A/I). Thirty A/I rats were randomly divided into untreated group, celebrex group and No.2RFR group. Another 10 SD rats were selected as normal control group. After 2-month treatment, the pathology of the nephridial tissue was observed with hematoxylin and eosin straining under a light microscope. Renal function including serum creatinine (SCr) and blood urea nitrogen (BUN) was determined by using an automatic biochemical analyzer. Expressions of COX-2 and COX-1 mRNAs in nephridial tissues were detected by reverse transcription-polymerase chain reaction (RT-PCR).Results: No.2RFR could significantly decrease the levels of SCr and BUN. Renal function and morphology of CRF rats were ameliorated and the expressions of COX-2 mRNA were decreased significantly in the No.2RFR group and the celebrex group, but the expressions of COX-1 mRNA had no differences among the four groups.Conclusion: No.2RFR can improve renal function and reduce glomerular sclerosis and renal fibrosis by inhibiting the over-expression of the COX-2 mRNA.