新疆农业科学
新疆農業科學
신강농업과학
XINJIANG AGRICULTURAL SCIENCES
2010年
1期
126-129
,共4页
张志东%谢玉清%楚敏%顾美英%宋素琴%唐琦勇%茆军
張誌東%謝玉清%楚敏%顧美英%宋素琴%唐琦勇%茆軍
장지동%사옥청%초민%고미영%송소금%당기용%묘군
山药%内生菌%分离%鉴定
山藥%內生菌%分離%鑒定
산약%내생균%분리%감정
yam rhizome%endophyte%isolation%identify
[目的和方法]分别采用5种培养基对山药根根中的内生菌进行分离,得到了10株内生菌,通过形态学分析其为4种微生物,16S rRNA比对分析确定其分属为欧文氏菌属(Erwinia)和芽孢杆菌属(Bacillus),未发现放线菌和真菌.[结果]表明山药内生菌极为单调.其中,能在山药浸出液培养基中大量产生多糖的菌株SS2的16S rRNA与模式菌株Erwinia pyrifoliae Ep1/96~T 最大同源性为97.1%.[结论]极有可能为新种.
[目的和方法]分彆採用5種培養基對山藥根根中的內生菌進行分離,得到瞭10株內生菌,通過形態學分析其為4種微生物,16S rRNA比對分析確定其分屬為歐文氏菌屬(Erwinia)和芽孢桿菌屬(Bacillus),未髮現放線菌和真菌.[結果]錶明山藥內生菌極為單調.其中,能在山藥浸齣液培養基中大量產生多糖的菌株SS2的16S rRNA與模式菌株Erwinia pyrifoliae Ep1/96~T 最大同源性為97.1%.[結論]極有可能為新種.
[목적화방법]분별채용5충배양기대산약근근중적내생균진행분리,득도료10주내생균,통과형태학분석기위4충미생물,16S rRNA비대분석학정기분속위구문씨균속(Erwinia)화아포간균속(Bacillus),미발현방선균화진균.[결과]표명산약내생균겁위단조.기중,능재산약침출액배양기중대양산생다당적균주SS2적16S rRNA여모식균주Erwinia pyrifoliae Ep1/96~T 최대동원성위97.1%.[결론]겁유가능위신충.
[Objective and Method]Ten endophytes were isolated from yam rhizome, using five different isolation media. Four microbes were chosen for 16S rRNA gene sequence and systematic analysis.[Result]Results of 16S rRNA gene sequences blasting showed that the strains were within Erwinia genus and Bacillus genus, no actinomycete and fungus was found, which indicated that there was a poor diversity of endophytes in yam rhizome. The max homology of 16S rRNA of the produced polysaccharide strain SS2 product from yam rhizome leaching solution and model strain Erwinia pyrifoliae Ep1/96T was 97.1%.[Conclusion]which suggested the strain may be a novel species.
