中国中西医结合杂志
中國中西醫結閤雜誌
중국중서의결합잡지
CHINESE JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE
2010年
2期
141-145
,共5页
乙型肝炎%病毒性%中医体质%人类白细胞抗原%基因多态性
乙型肝炎%病毒性%中醫體質%人類白細胞抗原%基因多態性
을형간염%병독성%중의체질%인류백세포항원%기인다태성
hepatitis B%viral%Chinese medicine constitution type%human leukocyte antigen%polymorphism of gene
目的 观察中医体质类型与浙江地区汉族人群乙型肝炎病毒(HBV)感染不同临床状态及人类白细胞抗原(HLA)DQA1基因多态性的关系,探讨中医体质因素在慢性乙型肝炎发病中的作用.方法 临床收集慢性乙型肝炎(CHB,120例)、慢性HBV携带者(ASC,60例)、自限性HBV感染者(RHBS,60例)3组患者,前两组诊断均经肝活检证实.以王琦体质分类判定中医体质类型;聚合酶链反应序列特异性引物(PCR-SSP)法检测HLA-DQA1基因型,比较组间体质类型分布的差异及组间基因频率的差异.结果 (1) CHB组阴虚质、痰湿质的分布频率(20.0%、12.5%)显著高于RHBS组(6.7%、1.7%),平和质的分布频率(11.7%)显著低于RHBS组(31.7%),差异均有统计学意义(OR=3.500,95%CI:1.16-10.60;OR=8.400,95%CI:1.09-65.42;OR=0.161,95%CI:0.076-0.34;均P<0.05);(2)CHB组湿热质的分布频率(24.2%)显著高于ASC组(6.7%,P<0.05,OR=4.462,95%CI: 1.49-13.36),平和质的分布频率(11.7%)显著低于ASC组(45.0%,P<0.01,OR=0.285,95%CI:0.13-0.62);(3)HLA-DQA1*0201在CHB组的分布频率(38.3%)显著高于RHBS组(5.8%,P<0.01,OR=10.04,95%CI:4.48-22.48);HLA-DQA1*0102的分布频率(9.6%)显著低于RHBS组(36.7%,P<0.01,OR=0.183,95%CI:0.10-0.32);(4)HLA-DQA1*0201在CHB组的分布频率(38.3%)显著高于ASC组(7.5%,P<0.01,OR=7.667,95%CI:3.7-15.87);HLA-DQA1*0102的分布频率(20%)显著低于ASC组(9.6%,P<0.01,OR=0.424,95%CI:0.23-0.79).结论 中医体质因素和HLA-DQA1基因多态性均可影响HBV感染的临床结局,但其间关系需进一步研究明确.
目的 觀察中醫體質類型與浙江地區漢族人群乙型肝炎病毒(HBV)感染不同臨床狀態及人類白細胞抗原(HLA)DQA1基因多態性的關繫,探討中醫體質因素在慢性乙型肝炎髮病中的作用.方法 臨床收集慢性乙型肝炎(CHB,120例)、慢性HBV攜帶者(ASC,60例)、自限性HBV感染者(RHBS,60例)3組患者,前兩組診斷均經肝活檢證實.以王琦體質分類判定中醫體質類型;聚閤酶鏈反應序列特異性引物(PCR-SSP)法檢測HLA-DQA1基因型,比較組間體質類型分佈的差異及組間基因頻率的差異.結果 (1) CHB組陰虛質、痰濕質的分佈頻率(20.0%、12.5%)顯著高于RHBS組(6.7%、1.7%),平和質的分佈頻率(11.7%)顯著低于RHBS組(31.7%),差異均有統計學意義(OR=3.500,95%CI:1.16-10.60;OR=8.400,95%CI:1.09-65.42;OR=0.161,95%CI:0.076-0.34;均P<0.05);(2)CHB組濕熱質的分佈頻率(24.2%)顯著高于ASC組(6.7%,P<0.05,OR=4.462,95%CI: 1.49-13.36),平和質的分佈頻率(11.7%)顯著低于ASC組(45.0%,P<0.01,OR=0.285,95%CI:0.13-0.62);(3)HLA-DQA1*0201在CHB組的分佈頻率(38.3%)顯著高于RHBS組(5.8%,P<0.01,OR=10.04,95%CI:4.48-22.48);HLA-DQA1*0102的分佈頻率(9.6%)顯著低于RHBS組(36.7%,P<0.01,OR=0.183,95%CI:0.10-0.32);(4)HLA-DQA1*0201在CHB組的分佈頻率(38.3%)顯著高于ASC組(7.5%,P<0.01,OR=7.667,95%CI:3.7-15.87);HLA-DQA1*0102的分佈頻率(20%)顯著低于ASC組(9.6%,P<0.01,OR=0.424,95%CI:0.23-0.79).結論 中醫體質因素和HLA-DQA1基因多態性均可影響HBV感染的臨床結跼,但其間關繫需進一步研究明確.
목적 관찰중의체질류형여절강지구한족인군을형간염병독(HBV)감염불동림상상태급인류백세포항원(HLA)DQA1기인다태성적관계,탐토중의체질인소재만성을형간염발병중적작용.방법 림상수집만성을형간염(CHB,120례)、만성HBV휴대자(ASC,60례)、자한성HBV감염자(RHBS,60례)3조환자,전량조진단균경간활검증실.이왕기체질분류판정중의체질류형;취합매련반응서렬특이성인물(PCR-SSP)법검측HLA-DQA1기인형,비교조간체질류형분포적차이급조간기인빈솔적차이.결과 (1) CHB조음허질、담습질적분포빈솔(20.0%、12.5%)현저고우RHBS조(6.7%、1.7%),평화질적분포빈솔(11.7%)현저저우RHBS조(31.7%),차이균유통계학의의(OR=3.500,95%CI:1.16-10.60;OR=8.400,95%CI:1.09-65.42;OR=0.161,95%CI:0.076-0.34;균P<0.05);(2)CHB조습열질적분포빈솔(24.2%)현저고우ASC조(6.7%,P<0.05,OR=4.462,95%CI: 1.49-13.36),평화질적분포빈솔(11.7%)현저저우ASC조(45.0%,P<0.01,OR=0.285,95%CI:0.13-0.62);(3)HLA-DQA1*0201재CHB조적분포빈솔(38.3%)현저고우RHBS조(5.8%,P<0.01,OR=10.04,95%CI:4.48-22.48);HLA-DQA1*0102적분포빈솔(9.6%)현저저우RHBS조(36.7%,P<0.01,OR=0.183,95%CI:0.10-0.32);(4)HLA-DQA1*0201재CHB조적분포빈솔(38.3%)현저고우ASC조(7.5%,P<0.01,OR=7.667,95%CI:3.7-15.87);HLA-DQA1*0102적분포빈솔(20%)현저저우ASC조(9.6%,P<0.01,OR=0.424,95%CI:0.23-0.79).결론 중의체질인소화HLA-DQA1기인다태성균가영향HBV감염적림상결국,단기간관계수진일보연구명학.
Objective To observe the Chinese medicine constitution types and human leukocyte antigen (HLA)-DQA1 gene polymorphism in patients with hepatitis B (HB) virus infection in Chinese Han population of Zhejiang Province,for exploring the roles of constitution factor in pathogenesis of HB. Methods A total of 240 subjects,including 120 biopsy-proven chronic HB (CHB),60 HB asymptomatic carrier (ASC) and 60 resolved from HBV infection spontaneously (RHBS) were studied. Their Chinese medicine constitution type was judged by Wangqi's classification,and their genotype of HLA-DQA1 was detected by polymerase chain reaction sequence specific primer for comparing the difference between groups in distribution frequency (DF) of constitution types and genes.Results (1) As compared with the RHBS group,DF of yin-deficiency constitution and phlegm-dampness constitution in the CHB group was significant higher (20.0% vs 6.7% and 12.5% vs 1.7%),and that of placid constitution was significant lower (11.7% vs 31.7%),showing statistical significance between groups (OR=3.5,95%CI: 1.16-10.60;OR=8.4,95%CI: 1.09-65.42;OR=0.161,95%CI: 0.076-0.34;all P<0.05). (2) As compared with the ASC group,DF of damp-heat constitution was significant higher (24.2% vs 6.7%,P<0.05,OR=4.462,95%CI: 1.49-13.36),and that of placid constitution was significant lower (11.7% vs 45.0%,P<0.01,OR=0.285,95%CI: 0.13-0.62) in the CHB group. (3) As compared with RHBS group,DF of HLA-DQA1* 0201 allele in CHB group was significant higher (38.3% vs 5.8%,P<0.01,OR=10.04,95%CI: 4.48-22.48);and that of HLA-DQA1*0102 allele was significant lower (9.6% vs 36.7%,P<0.01,OR=0.183,95%CI: 0.10-0.32). (4) As compared with ASC group,DF of HLA-DQA1*0201 allele in CHB group was significant higher (38.3% vs 7.5%,P<0.01,OR=7.667,95%CI: 3.7-15.87),and that of HLA-DQA1*0102 allele was significant lower (20.0% vs 9.6%,P<0.01,OR=0.424,95%CI: 0.23-0.79). Conclusion Both Chinese medicine constitution and HLA-DQA1 gene polymorphism show connection with the outcomes of HB virus infection in Chinese Han population,but the real association between them is required for further study.
