中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2012年
3期
199-204
,共6页
李宝新%王燕%刘岩%马剑侠%李玉坤
李寶新%王燕%劉巖%馬劍俠%李玉坤
리보신%왕연%류암%마검협%리옥곤
糖尿病,2型%骨质疏松%受体,骨化三醇%成纤维细胞生长因子23%骨形态发生蛋白7
糖尿病,2型%骨質疏鬆%受體,骨化三醇%成纖維細胞生長因子23%骨形態髮生蛋白7
당뇨병,2형%골질소송%수체,골화삼순%성섬유세포생장인자23%골형태발생단백7
Diabetes mellitus,type 2%Osteoporosis%Receptors,calcitriol%Fibroblast growth factor 23%Bone morphogenetic protein 7
目的 探讨2型糖尿病并发骨质疏松大鼠肾和骨组织维生素D受体(VDR)、成纤维细胞生长因子(FGF)23、骨形态发生蛋白(BMP)7的表达及意义.方法 雌性Wistar大鼠随机分为假手术对照组(NS组)、单纯去卵巢组(NOVX组)、2型糖尿病假手术组(DS组)、2型糖尿病去卵巢组(D0VX 组).糖尿病组大鼠予高脂高糖饲料联合小剂量链脲佐菌素腹腔注射制备2型糖尿病模型,模型成功后去卵巢组行双侧卵巢切除术,去卵巢后4周、8周、12周末随机分批处死.用RT-PCR法观察各组肾和骨组织VDR、FGF23、BMP7的mRNA表达,Western免疫印迹法观察各组肾组织VDR、FGF23、BMP7蛋白表达.结果 成功制备2型糖尿病并发骨质疏松模型.随着时间延长,DS组和DOVX组肾和骨组织VDR、BMP7的mRNA表达和肾组织VDR、BMP7蛋白表达均较NS组和NOVX组降低,且以DOVX组表达最低(P＜0.05)；DS组和DOVX组骨组织FGF23的mRNA表达明显低于NS和NOVX组(0.566±0.059,0.452±0.057比1.008±0.068,0.520±0.049,P＜0.05),而DS组和DOVX组肾组织FGF23的mRNA和蛋白(1.034±0.071,1.136±0.112比0.919±0.086,0.952±0.143)表达明显高于NS和NOVX组,以DOVX组表达最高(P＜0.05).结论 FGF23和BMP7在肾和骨组织共同介导2型糖尿病并发骨质疏松大鼠维生素D受体表达中起不同作用.
目的 探討2型糖尿病併髮骨質疏鬆大鼠腎和骨組織維生素D受體(VDR)、成纖維細胞生長因子(FGF)23、骨形態髮生蛋白(BMP)7的錶達及意義.方法 雌性Wistar大鼠隨機分為假手術對照組(NS組)、單純去卵巢組(NOVX組)、2型糖尿病假手術組(DS組)、2型糖尿病去卵巢組(D0VX 組).糖尿病組大鼠予高脂高糖飼料聯閤小劑量鏈脲佐菌素腹腔註射製備2型糖尿病模型,模型成功後去卵巢組行雙側卵巢切除術,去卵巢後4週、8週、12週末隨機分批處死.用RT-PCR法觀察各組腎和骨組織VDR、FGF23、BMP7的mRNA錶達,Western免疫印跡法觀察各組腎組織VDR、FGF23、BMP7蛋白錶達.結果 成功製備2型糖尿病併髮骨質疏鬆模型.隨著時間延長,DS組和DOVX組腎和骨組織VDR、BMP7的mRNA錶達和腎組織VDR、BMP7蛋白錶達均較NS組和NOVX組降低,且以DOVX組錶達最低(P＜0.05)；DS組和DOVX組骨組織FGF23的mRNA錶達明顯低于NS和NOVX組(0.566±0.059,0.452±0.057比1.008±0.068,0.520±0.049,P＜0.05),而DS組和DOVX組腎組織FGF23的mRNA和蛋白(1.034±0.071,1.136±0.112比0.919±0.086,0.952±0.143)錶達明顯高于NS和NOVX組,以DOVX組錶達最高(P＜0.05).結論 FGF23和BMP7在腎和骨組織共同介導2型糖尿病併髮骨質疏鬆大鼠維生素D受體錶達中起不同作用.
목적 탐토2형당뇨병병발골질소송대서신화골조직유생소D수체(VDR)、성섬유세포생장인자(FGF)23、골형태발생단백(BMP)7적표체급의의.방법 자성Wistar대서수궤분위가수술대조조(NS조)、단순거란소조(NOVX조)、2형당뇨병가수술조(DS조)、2형당뇨병거란소조(D0VX 조).당뇨병조대서여고지고당사료연합소제량련뇨좌균소복강주사제비2형당뇨병모형,모형성공후거란소조행쌍측란소절제술,거란소후4주、8주、12주말수궤분비처사.용RT-PCR법관찰각조신화골조직VDR、FGF23、BMP7적mRNA표체,Western면역인적법관찰각조신조직VDR、FGF23、BMP7단백표체.결과 성공제비2형당뇨병병발골질소송모형.수착시간연장,DS조화DOVX조신화골조직VDR、BMP7적mRNA표체화신조직VDR、BMP7단백표체균교NS조화NOVX조강저,차이DOVX조표체최저(P＜0.05)；DS조화DOVX조골조직FGF23적mRNA표체명현저우NS화NOVX조(0.566±0.059,0.452±0.057비1.008±0.068,0.520±0.049,P＜0.05),이DS조화DOVX조신조직FGF23적mRNA화단백(1.034±0.071,1.136±0.112비0.919±0.086,0.952±0.143)표체명현고우NS화NOVX조,이DOVX조표체최고(P＜0.05).결론 FGF23화BMP7재신화골조직공동개도2형당뇨병병발골질소송대서유생소D수체표체중기불동작용.
Objective To investigate the expression and significance of Vitamin D receptor (VDR),fibroblast growth factor(FGF) 23 and bone morphogenetic protein(BMP) 7 in the kidney and bone tissues in rats with type 2 diabetes and osteoporosis.Methods Female Wistar rats were randomly divided into four groups:sham control group (NS group),ovariectomy group (NOVX group),type 2 diabetes sham surgery group (DS group) and type 2 diabetic rats undergoing ovariectomy group (DOVX group).In diabetes groups,rats were administrated with high-sugar-fat diet and intraperitoneal injection of small dose of streptozotocin so as to establish type 2 diabetes models (T2DMs).After the models were successfully established,the ovariectomy groups received ovariectomy and were randomly sacrificed at the end of week 4,8 and 12 after ovariectomy in batches.The mRNA expression levels of VDR,FGF23 and BMP7 in kidney and bone tissues in each group were observed by RT-PCR and the protein expression levels of VDR,FGF23 and BMP7 in kidney tissues by Western blot.Results The models of type 2 diabetes with osteoporosis were successfully established.Both mRNA expression levcls of VDR and BMP7 in kidney and bone tissues and the protein expression levels of VDR and BMP7 in kidney tissues of DS and DOVX groups were lower than those of NS and NOVX groups along with time (P＜0.05).DS and DOVX groups had significantly lower mRNA expression of FGF23 in bone tissues as compared with NS and NOVX groups(0.566±0.059,0.452±0.057 vs 1.008±0.068,0.520±0.049,P＜0.05),but the mRNA and protein expression of FGF23 in kidney tissues of DS and DOVX groups was significantly higher than that of NS and NOVX groups (protein:1.034±0.071,1.136±0.112 vs 0.919±0.086,0.952±0.143),with expression level in DOVX group being the highest(P＜ 0.05).Conclusion The expression of vitamin D receptor(VDR) mediated by both FGF23 and BMP7 may not exert effects on rats of type 2 diabetes with osteoporosis.
