中华消化外科杂志
中華消化外科雜誌
중화소화외과잡지
CHINESE JOURNAL OF DIGESTIVE SURGERY
2012年
3期
279-283
,共5页
卢鹏%李霄%窦科峰%党政%胡延忠%马远方%宋振顺
盧鵬%李霄%竇科峰%黨政%鬍延忠%馬遠方%宋振順
로붕%리소%두과봉%당정%호연충%마원방%송진순
肝肿瘤%热休克转录因子l%热休克蛋白70%免疫组织化学
肝腫瘤%熱休剋轉錄因子l%熱休剋蛋白70%免疫組織化學
간종류%열휴극전록인자l%열휴극단백70%면역조직화학
Liver neoplasms%Heat shock transcription factor 1%Heat shock protein 70%Immunohistochemistry
目的 探讨热休克转录因子1( HSF1)蛋白在人肝细胞癌组织中的表达及临床意义,并推测HSF1在肝细胞癌发生、发展中的可能分子机制.方法 收集2006年3月至2007年3月第四军医大学西京医院67例行手术切除的肝细胞癌组织,以及21例因肝血管瘤行手术切除的部分正常肝脏组织,采用免疫组织化学法柃测肝细胞癌及正常肝脏组织中HSF1蛋白及热休克蛋白70( HSP70)的表达,分析HSF1蛋白与患者临床病理特征及预后之间的关系,并根据HSFI蛋白与HSP70表达情况的相关性分析推测HSF1在肝细胞癌发生、发展中的可能分子机制.不同组织中HSF1蛋白阳性率的分析以及肝细胞癌中HSF1蛋白阳性表达率与各临床病理资料的关系采用x2检验及Fisher确切概率法,HSFI蛋白与HSP70在肝细胞癌中表达的相关性采用Spearman相关性分析.采用Kaplan-Meier法绘制生存曲线,生存情况比较采用Log-rank检验.结果 HSFI蛋白在肝细胞癌组织中的阳性率为69％ (46/67),在正常肝脏组织中的阳性率为29％ (6/21),两者比较,差异有统计学意义(x2=10.628,P＜0.05).HSP70在肝细胞癌组织中的阳性率为57％( 38/67),在正常肝脏组织中的阳性率为24％ (5/21),两者比较,差异有统计学意义(x2=6.929,P＜0.05).HSF1蛋白与HSP70在肝细胞癌组织中的表达呈正相关(r=0.319,P＜0.05).HSFI蛋白的高表达与肝细胞癌的包膜完整与否、分化程度以及TNM分期有关(X2=5.935,9.762,5.159,11.267,P＜0.05)；而与患者性别、年龄、HBsAg水平、AFP水平以及是否存在门静脉癌栓无关(x2=0.822,0.172,2.059,P＞0.05).HSF1蛋白阳性和阴性表达者的生存时间分别为(21.4+1.9)个月和(29.8±2.7)个月,两者生存率比较,差异有统计学意义(x2=4.276,P＜0.05).结论 HSF1与肝细胞癌的发生、发展、侵袭转移以及不良预后相关,其可能通过调控HSP70的表达而发挥作用,在肝细胞癌的诊断和治疗中有良好应用前景.
目的 探討熱休剋轉錄因子1( HSF1)蛋白在人肝細胞癌組織中的錶達及臨床意義,併推測HSF1在肝細胞癌髮生、髮展中的可能分子機製.方法 收集2006年3月至2007年3月第四軍醫大學西京醫院67例行手術切除的肝細胞癌組織,以及21例因肝血管瘤行手術切除的部分正常肝髒組織,採用免疫組織化學法柃測肝細胞癌及正常肝髒組織中HSF1蛋白及熱休剋蛋白70( HSP70)的錶達,分析HSF1蛋白與患者臨床病理特徵及預後之間的關繫,併根據HSFI蛋白與HSP70錶達情況的相關性分析推測HSF1在肝細胞癌髮生、髮展中的可能分子機製.不同組織中HSF1蛋白暘性率的分析以及肝細胞癌中HSF1蛋白暘性錶達率與各臨床病理資料的關繫採用x2檢驗及Fisher確切概率法,HSFI蛋白與HSP70在肝細胞癌中錶達的相關性採用Spearman相關性分析.採用Kaplan-Meier法繪製生存麯線,生存情況比較採用Log-rank檢驗.結果 HSFI蛋白在肝細胞癌組織中的暘性率為69％ (46/67),在正常肝髒組織中的暘性率為29％ (6/21),兩者比較,差異有統計學意義(x2=10.628,P＜0.05).HSP70在肝細胞癌組織中的暘性率為57％( 38/67),在正常肝髒組織中的暘性率為24％ (5/21),兩者比較,差異有統計學意義(x2=6.929,P＜0.05).HSF1蛋白與HSP70在肝細胞癌組織中的錶達呈正相關(r=0.319,P＜0.05).HSFI蛋白的高錶達與肝細胞癌的包膜完整與否、分化程度以及TNM分期有關(X2=5.935,9.762,5.159,11.267,P＜0.05)；而與患者性彆、年齡、HBsAg水平、AFP水平以及是否存在門靜脈癌栓無關(x2=0.822,0.172,2.059,P＞0.05).HSF1蛋白暘性和陰性錶達者的生存時間分彆為(21.4+1.9)箇月和(29.8±2.7)箇月,兩者生存率比較,差異有統計學意義(x2=4.276,P＜0.05).結論 HSF1與肝細胞癌的髮生、髮展、侵襲轉移以及不良預後相關,其可能通過調控HSP70的錶達而髮揮作用,在肝細胞癌的診斷和治療中有良好應用前景.
목적 탐토열휴극전록인자1( HSF1)단백재인간세포암조직중적표체급림상의의,병추측HSF1재간세포암발생、발전중적가능분자궤제.방법 수집2006년3월지2007년3월제사군의대학서경의원67례행수술절제적간세포암조직,이급21례인간혈관류행수술절제적부분정상간장조직,채용면역조직화학법령측간세포암급정상간장조직중HSF1단백급열휴극단백70( HSP70)적표체,분석HSF1단백여환자림상병리특정급예후지간적관계,병근거HSFI단백여HSP70표체정황적상관성분석추측HSF1재간세포암발생、발전중적가능분자궤제.불동조직중HSF1단백양성솔적분석이급간세포암중HSF1단백양성표체솔여각림상병리자료적관계채용x2검험급Fisher학절개솔법,HSFI단백여HSP70재간세포암중표체적상관성채용Spearman상관성분석.채용Kaplan-Meier법회제생존곡선,생존정황비교채용Log-rank검험.결과 HSFI단백재간세포암조직중적양성솔위69％ (46/67),재정상간장조직중적양성솔위29％ (6/21),량자비교,차이유통계학의의(x2=10.628,P＜0.05).HSP70재간세포암조직중적양성솔위57％( 38/67),재정상간장조직중적양성솔위24％ (5/21),량자비교,차이유통계학의의(x2=6.929,P＜0.05).HSF1단백여HSP70재간세포암조직중적표체정정상관(r=0.319,P＜0.05).HSFI단백적고표체여간세포암적포막완정여부、분화정도이급TNM분기유관(X2=5.935,9.762,5.159,11.267,P＜0.05)；이여환자성별、년령、HBsAg수평、AFP수평이급시부존재문정맥암전무관(x2=0.822,0.172,2.059,P＞0.05).HSF1단백양성화음성표체자적생존시간분별위(21.4+1.9)개월화(29.8±2.7)개월,량자생존솔비교,차이유통계학의의(x2=4.276,P＜0.05).결론 HSF1여간세포암적발생、발전、침습전이이급불량예후상관,기가능통과조공HSP70적표체이발휘작용,재간세포암적진단화치료중유량호응용전경.
Objective To investigate the expression and clinical significance of heat shock transcription factor 1 (HSF1) protein in human hepatocellular carcinoma (HCC) tissues,and deduce the probable molecular mechanism of HSF1 in the development and advancement of HCC.Methods Sixty-seven samples of HCC tissue and 21 samples of normal liver tissue were obtained from March 2006 to March 2007 at the Xijing Hospital.The expressions of HSF1 protein and heat shock protein 70 (HSP70) were detected by using immunohistochemistry.The probable molecular mechanism of HSF1 in the development and advancement of HCC was deduced according to the relationship between the expressions of HSF1 protein and HSP70.Positive rates of HSF1 protein in different tissues and the relationship between HSF1 protein expression in the HCC tissues and clinical pathological factors were analyzed by the chi-square test and by calculating Fisher exact probability,respectively.The correlation between the expressions of HSF1 protein and HSP70 in the HCC tissue was analyzed by the Spearman correlation coefficient.The survival curve was drawn by the Kaplan-Meier method,and the survival rate was analyzed by the Log-rank test.Results The positive rates of HSF1 protein expression was 69％ (46/67) in the HCC tissue,which was significantly higher than 29％ (6/21) in the normal liver tissue ( x2 =10.628,P ＜ 0.05 ),The positive rates of HSP70 expression in the HCC tissue was 57％ (38/67),which was significantly higher than 24％ (5/21) in the normal liver tissue ( x2 =6.929,P ＜ 0.05 ).The expression of HSF1 protein in the HCC tissue was positively correlated with that of HSP70 (r=0.319,P ＜0.05).The high expression of HSF1 protein was correlated with the integrity of capsule of HCC,tumor differentiation and TNM stage (x2 =5.935,9.762,5.159,11.267,P＜0.05 ),while the high expression of HSF1 protein was not correlated with the gender,age,levels of hepatitis B surface antigen and alpha fetoprotein,and portal vein tumor thrombus ( x2 =0.822,0.172,2.059,P ＞0.05 ).The survival time was (21.4 ± 1.9 )months for patients with positive HSF1 protein expression and (29.8 ± 2.7 ) months for patients with negative HSF1 protein expression.There was a significant difference in the survival time between patients with positive and negative HSF1 protein expression ( x2 =4.276,P ＜ 0.05 ).Conclusions HSF1 is correlated with the development,advancement,invasion,metastasis and malignant prognosis of HCC.HSF1 takes effects by regulating the expression of HSP70,and it has a good perspective of clinical application for the diagnosis and treatment of HCC.
