中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2012年
3期
187-190
,共4页
蒋毅%涂胜豪%夏玉坤%陈哲%常栋%杨宏伟%胡永红
蔣毅%塗勝豪%夏玉坤%陳哲%常棟%楊宏偉%鬍永紅
장의%도성호%하옥곤%진철%상동%양굉위%호영홍
模型,动物%关节炎,类风湿%受体,表皮生长因子%基因,ErbB-2%雷公藤多苷
模型,動物%關節炎,類風濕%受體,錶皮生長因子%基因,ErbB-2%雷公籐多苷
모형,동물%관절염,류풍습%수체,표피생장인자%기인,ErbB-2%뢰공등다감
Models,animal%Arthritis,rheumatoid%Receptor,epidermal growth factor%Gene,ErbB-2%Tripterygium wilfordiipolyglycoside
目的 观察表皮生长因子受体(EGFR)家族成员EGFR、ErbB-2在胶原诱导性关节炎(CIA)大鼠滑膜及软骨中的表达及雷公藤多苷(TWP)对其影响,探讨EGFR、ErbB-2在CIA大鼠发病中的,作用及TWP治疗RA的药理作用机制.方法 建立CIA大鼠模型,分别采用免疫组织化学染色及实时定量聚合酶链反应( PCR)检测EGFR、ErbB-2在滑膜和软骨中的表达.统计学处理采用单因素方差分析.结果 EGFR、ErbB-2的蛋白表达及mRNA水平在CIA模型组滑膜(EGFR 0.268±0.059,ErbB-2 0.25±0.04.EGFR mRNA:14.2±0.55,ErbB-2 mRNA 23.46±3.64)和软骨(EGFR 0.193±0.018,ErbB-2 0.217±0.033,EGFR mRNA:4.16±0.50,ErbB-2 mRNA 9.23±0.66)中明显高于正常组(P＜0.01),在治疗组中表达低于模型组(P＜0.01).结论 初步证明EGFR、ErbB-2参与了CIA的病理过程.TWP治疗RA的机制可能与其降低EGFR、ErbB-2的表达有关.
目的 觀察錶皮生長因子受體(EGFR)傢族成員EGFR、ErbB-2在膠原誘導性關節炎(CIA)大鼠滑膜及軟骨中的錶達及雷公籐多苷(TWP)對其影響,探討EGFR、ErbB-2在CIA大鼠髮病中的,作用及TWP治療RA的藥理作用機製.方法 建立CIA大鼠模型,分彆採用免疫組織化學染色及實時定量聚閤酶鏈反應( PCR)檢測EGFR、ErbB-2在滑膜和軟骨中的錶達.統計學處理採用單因素方差分析.結果 EGFR、ErbB-2的蛋白錶達及mRNA水平在CIA模型組滑膜(EGFR 0.268±0.059,ErbB-2 0.25±0.04.EGFR mRNA:14.2±0.55,ErbB-2 mRNA 23.46±3.64)和軟骨(EGFR 0.193±0.018,ErbB-2 0.217±0.033,EGFR mRNA:4.16±0.50,ErbB-2 mRNA 9.23±0.66)中明顯高于正常組(P＜0.01),在治療組中錶達低于模型組(P＜0.01).結論 初步證明EGFR、ErbB-2參與瞭CIA的病理過程.TWP治療RA的機製可能與其降低EGFR、ErbB-2的錶達有關.
목적 관찰표피생장인자수체(EGFR)가족성원EGFR、ErbB-2재효원유도성관절염(CIA)대서활막급연골중적표체급뢰공등다감(TWP)대기영향,탐토EGFR、ErbB-2재CIA대서발병중적,작용급TWP치료RA적약리작용궤제.방법 건립CIA대서모형,분별채용면역조직화학염색급실시정량취합매련반응( PCR)검측EGFR、ErbB-2재활막화연골중적표체.통계학처리채용단인소방차분석.결과 EGFR、ErbB-2적단백표체급mRNA수평재CIA모형조활막(EGFR 0.268±0.059,ErbB-2 0.25±0.04.EGFR mRNA:14.2±0.55,ErbB-2 mRNA 23.46±3.64)화연골(EGFR 0.193±0.018,ErbB-2 0.217±0.033,EGFR mRNA:4.16±0.50,ErbB-2 mRNA 9.23±0.66)중명현고우정상조(P＜0.01),재치료조중표체저우모형조(P＜0.01).결론 초보증명EGFR、ErbB-2삼여료CIA적병리과정.TWP치료RA적궤제가능여기강저EGFR、ErbB-2적표체유관.
Objective To study the regulatory effect of Tripterygium wilfprdii polyglycoside (TWP) on the expression of EGFR and ErbB-2 induced arthritis rats.The effect of TWP on arthritis was also explored.Methods After the model of CIA rats were established,the expression of EGFR and ErbB-2 in the synovium and articular cartilage were tested by immunohistochemical stain and real time PCR.ANOVA was used for statistical analysis.Results The protein and mRNA expression of EGFR and ErbB-2 in the synovium (EGFR 0.268±0.059,ErbB-2 0.25±0.04,EGFR mRNA:14.2±0.55,ErbB-2 mRNA 23.46±3.64) and articular cartilage (EGFR 0.193±0.018,ErbB-2 0.217±0.033,EGFR mRNA:4.16±0.50,ErbB-2 mRNA 9.23±0.66) of the model group were significantly higher than those of the control group (P＜0.01).After being treated with TWP and MTX,the protein and mRNA expression of the EGFR and ErbB-2 decreased markedly (P＜0.01).Conclusion EGFR and ErbB-2 may play an important role in the pathogenesis of arthritis development.The molecular mechanism that TWP can treat synovitis and bone destruction of RA is related to the inhibition of EGFR and ErbB-2.
