CAJ | 학술논문

目的探讨超声造影剂SonoVue在诊断超声不同剂量、不同成像模式条件下对兔睾丸生精细胞的影响.方法选用大白兔33只,随机分组.对照组3只,双侧睾丸接受灰阶超声辐射3 min.常规造影组18只,分为A1、B1、C1、A2、B2、C2共6个小组,每组各3只,经兔耳缘静脉团注SonoVue,其中A1、A2组分别单次注射0.1 ml/kg;B1、B2组每次均注射0.1 ml/kg,间隔15 min重复2次;C1、C2组单次注射1 ml/kg.造影剂爆破组12只,分为D1、D2、E1、E2组,每组各3只,当造影剂刚充满整个睾丸实质时,单次触发爆破,其中D1、D2组注射0.1 ml/kg,E1、E2组注射1 ml/kg.A1、B1、C1、D1、E1各组于实验结束后立即取材,A2、B2、C2、D2、E2各组于实验结束常规饲养24 h后取材.应用光镜、电镜观察各组睾丸组织学变化.结果造影剂爆破组的凋亡细胞总数较对照组、常规造影组显著增多(P＜0.001),常规造影组与对照组的差异无统计学意义(P＞0.05).电镜下,常规造影组中除C1组睾丸内局部细胞间隙增宽、线粒体肿胀外,其他各小组均未见明显改变.造影剂爆破组的睾丸实质均可见细胞间隙增宽、线粒体空泡,其中D1组、E1组睾丸组织还存在点状坏死灶.结论常规造影组常规剂量对生精细胞无明显影响,大剂量可造成生精细胞的短暂的可逆性损伤.造影剂爆破可造成生精细胞不可逆的损伤.
목적 탐토초성조영제SonoVue재진단초성불동제량、불동성상모식조건하대토고환생정세포적영향.방법 선용대백토33지,수궤분조.대조조3지,쌍측고환접수회계초성복사3 min.상규조영조18지,분위A1、B1、C1、A2、B2、C2공6개소조,매조각3지,경토이연정맥단주SonoVue,기중A1、A2조분별단차주사0.1 ml/kg;B1、B2조매차균주사0.1 ml/kg,간격15 min중복2차;C1、C2조단차주사1 ml/kg.조영제폭파조12지,분위D1、D2、E1、E2조,매조각3지,당조영제강충만정개고환실질시,단차촉발폭파,기중D1、D2조주사0.1 ml/kg,E1、E2조주사1 ml/kg.A1、B1、C1、D1、E1각조우실험결속후립즉취재,A2、B2、C2、D2、E2각조우실험결속상규사양24 h후취재.응용광경、전경관찰각조고환조직학변화.결과 조영제폭파조적조망세포총수교대조조、상규조영조현저증다(P＜0.001),상규조영조여대조조적차이무통계학의의(P＞0.05).전경하,상규조영조중제C1조고환내국부세포간극증관、선립체종창외,기타각소조균미견명현개변.조영제폭파조적고환실질균가견세포간극증관、선립체공포,기중D1조、E1조고환조직환존재점상배사조.결론 상규조영조상규제량대생정세포무명현영향,대제량가조성생정세포적단잠적가역성손상.조영제폭파가조성생정세포불가역적손상.
Objective To study the impact of ultrasound contrast agent with different doses or imaging modes on rabbit spermatogenic cell in the diagnosis of radiation dose.Methods Thirty-three New Zealand white rabbits were randomly divided into 3 groups.In group Ⅰ ,three minutes gray scale ultrasound radiation was performed for three rabbits.In group Ⅱ, SonoVue was bolus injected from ear vein for eighteen rabbits.The rabbits in this group were divided into 6 sub-group according to different doses and different sampling times, the single injections of SonoVue with dosage of 0.1 ml/kg(A1, A2) or 1 ml/kg (C1 ,C2) and repeated injections with dosage of 0.1 ml/kg interval 15 min (B1 ,B2) were taken.In group Ⅲ ,twelve rabbits were bolus injected with SonoVue and then the agent bubbles were blasted when the SonoVue suffused the whole testis.The rabbits in this group were divided into 4 sub-groups according to different doses and different sampling times, and the dosages of SonoVue were 0.1 ml/kg(D1, D2) and 1 ml/kg(E1,E2).Testis were drew immediately or 24 hours later for the observation under light microscope and transmission electron microscope.Results Apoptotic cells in group Ⅲ were more than those in group Ⅰ and Ⅱ ( P ＜0.05) ,while no significant difference of the total number of apoptotic cells between group Ⅰ and Ⅱ ( P ＞0.05).The appearances of focal ultra-structural damage were observed by transmission electron microscope in group C1, such as intercellular space widening and mitochondria swelling, and no distinct damage were observed in the other groups.The appearances of intercellular space widening, mitochondria swelling and cellular edema were observed by transmission electron microscope in group Ⅲ, and spotty necrosisetc was also observed in group D1 and E1.Conclusions Large dose of contrast agent could cause germ cells slight instantaneous effects, while conventional dose had no effect.Contrast agent blasting may cause germ cells unrecoverable damage.