中华创伤骨科杂志
中華創傷骨科雜誌
중화창상골과잡지
CHINESE JOURNAL OF ORTHOPAEDIC TRAUMA
2011年
2期
175-177
,共3页
脑损伤%创伤和损伤%骨折%骨痂%基因表达
腦損傷%創傷和損傷%骨摺%骨痂%基因錶達
뇌손상%창상화손상%골절%골가%기인표체
Brain injuries%Wounds and injuries%Fractures%Bony callus%Gene expression
目的 通过对SD大鼠脑外伤合并四肢骨折不同时间段骨痂局部转化生长因子(TGF-β)的基因表达进行检测，了解TGF-β在增强骨折愈合过程中的作用。方法 纯种SD雄性大鼠80只，随机分为（每组40只）单纯四肢骨折组（对照组）与脑外伤合并四肢骨折组（实验组）（每组40只），两组均利用逆转录PCR基因扩增技术对骨折后l、2、3、4周骨痂局部TGF-β基因表达的改变进行检测。结果 与对照组相比，实验组在骨折后1周TGF-β的基因表达明显升高，差异有统计学意义(P＜0.05)；在骨折后3周其表达明显下降，差异有统计学意义(P＜0.05)；两组TGF-β基因表达均在骨折后2周达到峰值，峰值与其他时间段比较差异均有统计学意义(P＜0.05)，但两组间峰值比较差异无统计学意义(P＞0.05)。结论 脑外伤合并四肢骨折骨痂局部TGF-β基因表达模式的改变表明，TGF-β基因参与了增强的骨折愈合病理生理过程。
目的 通過對SD大鼠腦外傷閤併四肢骨摺不同時間段骨痂跼部轉化生長因子(TGF-β)的基因錶達進行檢測，瞭解TGF-β在增彊骨摺愈閤過程中的作用。方法 純種SD雄性大鼠80隻，隨機分為（每組40隻）單純四肢骨摺組（對照組）與腦外傷閤併四肢骨摺組（實驗組）（每組40隻），兩組均利用逆轉錄PCR基因擴增技術對骨摺後l、2、3、4週骨痂跼部TGF-β基因錶達的改變進行檢測。結果 與對照組相比，實驗組在骨摺後1週TGF-β的基因錶達明顯升高，差異有統計學意義(P＜0.05)；在骨摺後3週其錶達明顯下降，差異有統計學意義(P＜0.05)；兩組TGF-β基因錶達均在骨摺後2週達到峰值，峰值與其他時間段比較差異均有統計學意義(P＜0.05)，但兩組間峰值比較差異無統計學意義(P＞0.05)。結論 腦外傷閤併四肢骨摺骨痂跼部TGF-β基因錶達模式的改變錶明，TGF-β基因參與瞭增彊的骨摺愈閤病理生理過程。
목적 통과대SD대서뇌외상합병사지골절불동시간단골가국부전화생장인자(TGF-β)적기인표체진행검측，료해TGF-β재증강골절유합과정중적작용。방법 순충SD웅성대서80지，수궤분위（매조40지）단순사지골절조（대조조）여뇌외상합병사지골절조（실험조）（매조40지），량조균이용역전록PCR기인확증기술대골절후l、2、3、4주골가국부TGF-β기인표체적개변진행검측。결과 여대조조상비，실험조재골절후1주TGF-β적기인표체명현승고，차이유통계학의의(P＜0.05)；재골절후3주기표체명현하강，차이유통계학의의(P＜0.05)；량조TGF-β기인표체균재골절후2주체도봉치，봉치여기타시간단비교차이균유통계학의의(P＜0.05)，단량조간봉치비교차이무통계학의의(P＞0.05)。결론 뇌외상합병사지골절골가국부TGF-β기인표체모식적개변표명，TGF-β기인삼여료증강적골절유합병리생리과정。
Objective To investigate gene expression of transforming growth factor-β(TGF-β) in local bony callus in traumatic brain injury combined with extremity long bone fracture in rats.Methods Eighty male SD rats were randomized into 2 even groups: the experimental group were models of traumatic brain injury combined with extremity bone fracture and the control group were models of simple extremity bone fracture. Samples of bony callus were harvested at 1, 2, 3, and 4 weeks in both groups, each time from 10rats respectively, for detection of gene expressions of TGF-β by reverse transcription PCR techniques.Results Levels of gene expression of TGF-3 in local bony callus in the experimental group were significantly higher than in the control group at one week ( P ＜ 0. 05); but significantly lower at 3 weeks( P ＜ 0. 05) .Peak values occurred at 2 week in both groups, though, significantly higher than at other times, there was no significant difference between the 2 groups ( P ＞ 0. 05) .Conclusion Changes in gene expression of TGF-β in local bony callus in traumatic brain injury combined with extremity long bone fracture indicate that TGF-β may play a role in the process of increased fracture healing.
