CAJ | 학술논문

目的探讨膀胱癌中上皮-间质转化因子Twist表达及其与上皮-间质转化及病理参数之间的关系.方法应用E-钙黏蛋白和N-钙黏蛋白的表达来检测上皮-间质转化的发生.采用免疫组织化学SABC法、实时定量聚合酶链反应(PCR)和Western blot法观察45例膀胱癌组织和5例正常膀胱组织中Twist蛋白、E-钙黏蛋白和N-钙黏蛋白的表达.结果 45例膀胱癌组织和5例正常膀胱黏膜中Twist阳性率分别为26.66%和0,随着膀胱癌的病理分级及临床分期的增高,Twist的表达率逐渐升高.且Twist在有淋巴转移中的表达明显高于无淋巴结转移的膀胱癌.Twist的表达与E-钙黏蛋白和N-钙黏蛋白的表达显著相关.结论 Twist蛋白的表达对上皮-间质转化有重要影响,可为膀胱癌侵袭转移的研究和患者的预后判断提供参考.
목적 탐토방광암중상피-간질전화인자Twist표체급기여상피-간질전화급병리삼수지간적관계.방법 응용E-개점단백화N-개점단백적표체래검측상피-간질전화적발생.채용면역조직화학SABC법、실시정량취합매련반응(PCR)화Western blot법관찰45례방광암조직화5례정상방광조직중Twist단백、E-개점단백화N-개점단백적표체.결과 45례방광암조직화5례정상방광점막중Twist양성솔분별위26.66%화0,수착방광암적병리분급급림상분기적증고,Twist적표체솔축점승고.차Twist재유림파전이중적표체명현고우무림파결전이적방광암.Twist적표체여E-개점단백화N-개점단백적표체현저상관.결론 Twist단백적표체대상피-간질전화유중요영향,가위방광암침습전이적연구화환자적예후판단제공삼고.
Objective To investigate the relaitonship between the immunohistochemical expression of Twist and epithelial-mesenchymal transition in bladder cancer. Methods The epithelial-mesenchymal transition was assessed by the expression of E-cadherin and N-cadherin. The expression of E-cad-herin and N-cadherin in formalin-fixed and paraffin-embedded tissues and fresh tissue blocks from 49 patients with bladder cancer was detected by immunohistochemistry, real-time polymerase chain reaction (PCR) and Western blotting. Results The expression of Twist protein was detected in 14 cases (26.66% ) of bladder cancer. Twist positive rate was associated with the pathological parameters. The expression of Twist was closely associated with epithelial-mesenchymal transition and infiltration and metastasis. Conclusion Twist has an effect on epithelial-mesenchymal transition in bladder cancer. Twist may play an important role in the infiltration and metastasis of bladder cancer.