CAJ | 학술논문

目的了解绍兴市无偿献血者隐匿性乙肝病毒感染情况及病毒的分子生物学特征.方法应用ELISA的方法对绍兴市中心血站8692例无偿献血者进行筛查,对HBsAg阴性标本进行HBV DNA检测,从而检出隐匿性乙肝病毒感染者,再对阳性标本进行序列分析和氨基酸突变分析,从而了解绍兴市无偿献血者隐匿性乙肝病毒感染情况和病毒分子生物学特征,探讨隐匿性乙肝病毒感染的可能机制.结果在总共8692例无偿献血者标本中,共计H BsAg(-)8644例,在8644例HBsAg(-)标本中,有8例HBV DNA阳性,隐匿性HBV感染比例为0.92‰(8/8692),其中基因C型6例(75％),基因B型2例(25％)；氨基酸突变分析显示有7株OBI病毒株在“a”表位发生特异性突变.结论绍兴市无偿献血者中存在一定比例隐匿性乙肝病毒感染,隐匿性乙肝病毒感染与病毒基因突变有相关性.
목적 료해소흥시무상헌혈자은닉성을간병독감염정황급병독적분자생물학특정.방법 응용ELISA적방법대소흥시중심혈참8692례무상헌혈자진행사사,대HBsAg음성표본진행HBV DNA검측,종이검출은닉성을간병독감염자,재대양성표본진행서렬분석화안기산돌변분석,종이료해소흥시무상헌혈자은닉성을간병독감염정황화병독분자생물학특정,탐토은닉성을간병독감염적가능궤제.결과 재총공8692례무상헌혈자표본중,공계H BsAg(-)8644례,재8644례HBsAg(-)표본중,유8례HBV DNA양성,은닉성HBV감염비례위0.92‰(8/8692),기중기인C형6례(75％),기인B형2례(25％)；안기산돌변분석현시유7주OBI병독주재“a”표위발생특이성돌변.결론 소흥시무상헌혈자중존재일정비례은닉성을간병독감염,은닉성을간병독감염여병독기인돌변유상관성.
Objective To assess the molecular biological characteristics of occult hepatitis B virus ( HBV ) infected blood donors in Shaoxing.Methods 8692 blood donors were screened using ELISA.The occult HBV infection was determined by DNA analysis among the HBsAg negative subjects.DNA sequencing and mutational analysis were further performed in the HBV DNA positive samples.The overall situation of occult HBV infection was hereby evaluated and the possible underlying mechanisms discussed.Results Among the 8644 HBsAg negative subjects out of 8692 blood donors,8 were HBV DNA positive.The occult HBV infection rate was 0.92‰(8/8692).Among the 8 samples,6 were genotype C (75％) and 2 genotype B (25％).In addition,a specific mutation in "a" epitope was observed in 7OBI virus strains by amino acid analysis.Conclusion There were occult HBV infected among blood donors in Shaoxing,which is probably associated with the gene mutation of HBV virus.