CAJ | 학술논문

目的探讨雄性大鼠去势后血管内皮的损伤.方法 8周龄SD雄性大鼠30只随机分为3组:对照组(假去势)8只;去势组11只;去势后雄性激素替代治疗组11只.10周后,取主动脉血管,观察血管形态学变化和内皮细胞超微结构改变;用TUNEL法检测血管内皮细胞凋亡情况;RT-PCR检测bcl-2 mRNA表达情况.结果与正常对照组相比去势组主动脉内皮结构排列紊乱,内皮细胞结构不完整,有脱落现象;替代组动脉内膜病变明显较去势组减轻.TUNEL检测结果示与对照组相比,去势后大鼠主动脉内皮细胞凋亡数量(61％±20％)较对照组(24％±16％)明显增多(P＜0.05).bcl-2 mRNA相对表达量在去势组(0.59±0.09)较对照组明显下调(P＜0.05),而替代组(0.74±0.24)与对照组间差异无统计学意义(P＞0.05).结论雄性大鼠去势后,体内雄激素低于生理水平时,血管内皮结构损伤明显.
목적 탐토웅성대서거세후혈관내피적손상.방법 8주령SD웅성대서30지수궤분위3조:대조조(가거세)8지;거세조11지;거세후웅성격소체대치료조11지.10주후,취주동맥혈관,관찰혈관형태학변화화내피세포초미결구개변;용TUNEL법검측혈관내피세포조망정황;RT-PCR검측bcl-2 mRNA표체정황.결과 여정상대조조상비거세조주동맥내피결구배렬문란,내피세포결구불완정,유탈락현상;체대조동맥내막병변명현교거세조감경.TUNEL검측결과시여대조조상비,거세후대서주동맥내피세포조망수량(61％±20％)교대조조(24％±16％)명현증다(P＜0.05).bcl-2 mRNA상대표체량재거세조(0.59±0.09)교대조조명현하조(P＜0.05),이체대조(0.74±0.24)여대조조간차이무통계학의의(P＞0.05).결론 웅성대서거세후,체내웅격소저우생리수평시,혈관내피결구손상명현.
Objective To explore the injury of vascular endothelium in male castrated rats.Methods A total of 30 eight-week-old adult male SD rats were randomly divided into 3 groups:control ( n =8 ) ; castration ( n =11 ) ( low androgen) and replacement ( n =11 ) ( androgen dosing after castration).After 10 weeks of treatment or castration,the rats were sacrificed to obtain aortic specimens.Arterial morphological changes were observed by hematoxylin and eosin staining and electron microscope.Endothelial cell chromatin degradation was detected by the assay of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL).The mRNA level of bcl-2 was analyzed by reverse transcription-polymerase chain reaction (RT-PCR).Results Compared with the control group,the arterial structure was disorderly and had shedding of endothelium in the castration group.More apoptotic endothelial cells were found in the castration group(61％ ±20％ )than the other two groups (P ＜0.05).Compared with the control group,the bcl-2 mRNA level decreased significantly in the castration group ( P ＜ 0.05 ).However no significant difference existed in the replacement group ( P ＞ 0.05 ).Conclusion Low androgen may damage the structure of vascular endothelium in male castrated rats.