CAJ | 학술논문

目的观察人源脑肿瘤干细胞(hBTSC)在免疫缺陷动物脑内高致瘤性及高侵袭性.方法将原发和复发1 ×105个细胞的hBTSC球接种到40只裸小鼠脑内,移植瘤的组织学特点,免疫组织化学方法检测移植瘤中基质金属蛋白酶-1(MMP-1)的表达.结果 40只裸小鼠在30 d内全部成瘤.两种hBTSC在接种部位呈浸润性生长的同时,还散布于其他区域.但侵袭形式不尽相同,原发者在接种侧半球,呈团块,局限于脑实质;复发者,还浸润软脑膜和对侧半球.复发者MMPl+细胞的分布比原发者密集.结论 hBTSC除了高致瘤性,还具高侵袭性.复发hBTSC的侵袭性更强,可能与MMPl高表达有关.
목적 관찰인원뇌종류간세포(hBTSC)재면역결함동물뇌내고치류성급고침습성.방법 장원발화복발1 ×105개세포적hBTSC구접충도40지라소서뇌내,이식류적조직학특점,면역조직화학방법검측이식류중기질금속단백매-1(MMP-1)적표체.결과 40지라소서재30 d내전부성류.량충hBTSC재접충부위정침윤성생장적동시,환산포우기타구역.단침습형식불진상동,원발자재접충측반구,정단괴,국한우뇌실질;복발자,환침윤연뇌막화대측반구.복발자MMPl+세포적분포비원발자밀집.결론 hBTSC제료고치류성,환구고침습성.복발hBTSC적침습성경강,가능여MMPl고표체유관.
Objective To investigate the high oncogenicity and invasiveness of human brain tumor stem cell (hBTSC) transplantation tumor.Methods Primary and recurrent hBTSC spheres were injected into the brain of 40 nude mice. Histological characteristics and matrix metalloproteinase-1 ( MMP1 ) protein in the xenografts were determinated by HE staining and immunohistochemical method,respectively.Results All 40 injected nude mice formed tumor in 30 days.Xenografts showed invasive growth in injected location and spread other area.The invision of primary and recurrent xenografts was different.Primary xenografts were concentrated in injected hemicerebrum.Recurrent xenografts displayed dissemination growth,even to reach hemicerebrum and cerebral pia mater of opposite side.MMP1 + cells in primary xenografts were more than those in recurrent xenografts.Conclusion hBTSCs have strong oncogenicity and invasiveness.The stronger invasiveness of recurrent hBTSCs may be related to high expression of MMP1.