中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2012年
1期
44-48
,共5页
食管肿瘤%肿瘤,鳞状细胞%死亡相关蛋白激酶1%CD147%预后
食管腫瘤%腫瘤,鱗狀細胞%死亡相關蛋白激酶1%CD147%預後
식관종류%종류,린상세포%사망상관단백격매1%CD147%예후
Esophageal neoplasms%Neoplasms,squamous cell%Death associated protein kinase 1%CD147%Prognosis
目的 探讨死亡相关蛋白激酶1(DAPK1)、CD147在食管鳞癌中的表达状态及其与食管鳞癌预后的关系.方法 应用免疫组织化学方法和逆转录聚合酶链反应检测DAPK1、CD147在食管鳞癌和正常食管上皮组织中的表达水平,结合临床病理特征进行统计学分析.结果 DAPK1蛋白在食管鳞癌组织及癌旁正常组织中的阳性表达率分别为31.3％和58.5％,CD147蛋白的表达分别为57.5％和25.0％,差异均有统计学意义(均P＜0.01).不同浸润深度、淋巴结转移状态、TNM分期、分化程度的食管鳞癌患者DAPK1蛋白表达情况不同,差异有统计学意义(均P＜0.01)；不同浸润深度、淋巴结转移、TNM分期、分化程度的食管鳞癌患者CD147蛋白表达情况不同,差异有统计学意义(均P＜0.01).DAPK1 mRNA在52例食管鳞癌组织及癌旁正常组织中的表达水平分别为0.236±0.049和0.395±0.058,CD147 mRNA的表达水平分别为0.942±0.204和0.821±0.171,差异均有统计学意义(均P ＜0.01).在无淋巴结转移、分化程度高、病理分期低的食管鳞癌组织中DAPK1mRNA表达水平高；在淋巴结转移、分化程度低、病理分期高的食管鳞癌组织中CD147 mRNA表达水平高.结论 DAPK1、CD147蛋白的表达与食管鳞癌的临床病理学特征有关,DAPK1、CD147可能参与了食管鳞癌的转移过程,DAPK1、CD147作为预测食管鳞癌预后的重要指标.
目的 探討死亡相關蛋白激酶1(DAPK1)、CD147在食管鱗癌中的錶達狀態及其與食管鱗癌預後的關繫.方法 應用免疫組織化學方法和逆轉錄聚閤酶鏈反應檢測DAPK1、CD147在食管鱗癌和正常食管上皮組織中的錶達水平,結閤臨床病理特徵進行統計學分析.結果 DAPK1蛋白在食管鱗癌組織及癌徬正常組織中的暘性錶達率分彆為31.3％和58.5％,CD147蛋白的錶達分彆為57.5％和25.0％,差異均有統計學意義(均P＜0.01).不同浸潤深度、淋巴結轉移狀態、TNM分期、分化程度的食管鱗癌患者DAPK1蛋白錶達情況不同,差異有統計學意義(均P＜0.01)；不同浸潤深度、淋巴結轉移、TNM分期、分化程度的食管鱗癌患者CD147蛋白錶達情況不同,差異有統計學意義(均P＜0.01).DAPK1 mRNA在52例食管鱗癌組織及癌徬正常組織中的錶達水平分彆為0.236±0.049和0.395±0.058,CD147 mRNA的錶達水平分彆為0.942±0.204和0.821±0.171,差異均有統計學意義(均P ＜0.01).在無淋巴結轉移、分化程度高、病理分期低的食管鱗癌組織中DAPK1mRNA錶達水平高；在淋巴結轉移、分化程度低、病理分期高的食管鱗癌組織中CD147 mRNA錶達水平高.結論 DAPK1、CD147蛋白的錶達與食管鱗癌的臨床病理學特徵有關,DAPK1、CD147可能參與瞭食管鱗癌的轉移過程,DAPK1、CD147作為預測食管鱗癌預後的重要指標.
목적 탐토사망상관단백격매1(DAPK1)、CD147재식관린암중적표체상태급기여식관린암예후적관계.방법 응용면역조직화학방법화역전록취합매련반응검측DAPK1、CD147재식관린암화정상식관상피조직중적표체수평,결합림상병리특정진행통계학분석.결과 DAPK1단백재식관린암조직급암방정상조직중적양성표체솔분별위31.3％화58.5％,CD147단백적표체분별위57.5％화25.0％,차이균유통계학의의(균P＜0.01).불동침윤심도、림파결전이상태、TNM분기、분화정도적식관린암환자DAPK1단백표체정황불동,차이유통계학의의(균P＜0.01)；불동침윤심도、림파결전이、TNM분기、분화정도적식관린암환자CD147단백표체정황불동,차이유통계학의의(균P＜0.01).DAPK1 mRNA재52례식관린암조직급암방정상조직중적표체수평분별위0.236±0.049화0.395±0.058,CD147 mRNA적표체수평분별위0.942±0.204화0.821±0.171,차이균유통계학의의(균P ＜0.01).재무림파결전이、분화정도고、병리분기저적식관린암조직중DAPK1mRNA표체수평고；재림파결전이、분화정도저、병리분기고적식관린암조직중CD147 mRNA표체수평고.결론 DAPK1、CD147단백적표체여식관린암적림상병이학특정유관,DAPK1、CD147가능삼여료식관린암적전이과정,DAPK1、CD147작위예측식관린암예후적중요지표.
Objective To explore the amplification and expression status of DAPK1 and CD147 in esophageal squamous cell carcinoma ( ESCC ) and their relationship with the prognosis of ESCC.Methods Immunohistochemical staining and RT-PCR were used to detect the expression and amplification of DAPK1and CD147 in esophageal squamous carcinoma tissue and normal esophageal mucosa.Statistical analysis of the clinocopathological data was performed with SPSS 11.5 software package.Results The positive rates of expression of DAPK1 protein and CD147 protein in the specimens of esophageal carcinoma were 31.3％ and 58.5％,and in normal esophageal mucosa 57.5％ and 25.0％,respectively,with a statistically significant difference (P ＜ 0.001 ).The expressions of DAPK1 and CD147 were significant correlated with invasion depth,lymph node metastasis,TNM stage and the degree of cancer differentiation. ( P ＜ 0.05 ).The negative expression of DAPK1 and positive expression of CD147 indicated a poor prognosis.In 52 ESCC cases,the expression of DAPK1 in cancer tissues was 0.236 ± 0.049,and 0.395 ± 0.058 in normal esophageal mucosa,while that of CD147 mRNA expression was 0.942 ±0.204 and 0.821 ± 171,respectively,statistically both with a very significant difference ( P ＜ 0.01 ).There was a higher expression level of DAPK1 mRNA in the cancer tissue in patients with no lymph node metastasis,well differentiation,and earlier pathological stage,and a higher expression level of CD147 mRNA in the cancer tissues in patients with lymph node metastasis,poor differentiation,and later pathological stage.Conclusions The expression of DAPK1 and CD147 proteins is closely correlated with the clinicopathological characteristics of ESCC.The genes DAPK1 and CD147 may participate in the metastasis and apoptosis of ESCC.The expression of DAPK1 and CD147 may be used as important prognostic predictors in ESCC.
