CAJ | 학술논문

目的探讨环氧合酶-2(COX-2)、基质金属蛋白酶-9(MMP-9)及核增殖抗原(Ki67)在胃癌中的表达与胃癌发生、浸润和转移的关系.方法选择2003年1月至2005年12月手术切除、病理证实的胃癌存档蜡块标本58例,其中男37例,女21例.年龄31～76岁,中位年龄58.2岁.另取上述胃癌根治术患者距肿瘤5～6 cm的癌旁组织作对照.采用免疫组化法检测胃癌组织中COX2、MMP-9、Ki67的表达.结果 COX-2、MMP-9在胃癌组织中的表达率分别为82.76%和68.9%,均高于对照组(37.93%和24.14%,P＜0.01).COX-2、MMP-9的表达与胃癌患者性别、年龄、部位及大小无相关性(P＞0.05),与胃癌浸润深度、淋巴结转移及TNM分期有关(P＜0.05),MMP-9还与胃癌分化程度相关(P＜0.05).COX-2与MMP-9在胃癌组织中的表达有相关性(P＜0.05,C=0.359).MMP-9、COX-2表达阳性者Ki67表达水平高于阴性者,两者间差异有统计学意义(P＜0.01).结论 COX-2、MMP-9、Ki67在胃癌浸润、转移过程中起重要作用,共同促进肿瘤的发生、发展.
목적 탐토배양합매-2(COX-2)、기질금속단백매-9(MMP-9)급핵증식항원(Ki67)재위암중적표체여위암발생、침윤화전이적관계.방법 선택2003년1월지2005년12월수술절제、병리증실적위암존당사괴표본58례,기중남37례,녀21례.년령31～76세,중위년령58.2세.령취상술위암근치술환자거종류5～6 cm적암방조직작대조.채용면역조화법검측위암조직중COX2、MMP-9、Ki67적표체.결과 COX-2、MMP-9재위암조직중적표체솔분별위82.76%화68.9%,균고우대조조(37.93%화24.14%,P＜0.01).COX-2、MMP-9적표체여위암환자성별、년령、부위급대소무상관성(P＞0.05),여위암침윤심도、림파결전이급TNM분기유관(P＜0.05),MMP-9환여위암분화정도상관(P＜0.05).COX-2여MMP-9재위암조직중적표체유상관성(P＜0.05,C=0.359).MMP-9、COX-2표체양성자Ki67표체수평고우음성자,량자간차이유통계학의의(P＜0.01).결론 COX-2、MMP-9、Ki67재위암침윤、전이과정중기중요작용,공동촉진종류적발생、발전.
Objective To investigate the association of expressions of cyclooxygenase-2(COX-2),matrix metanoproteinases-9(MMp-9)and Ki67 with occurrence,infiltration and metastasis of gastric cancer.Methods Fifty-eight surgically obtained and pathologically performed cancerous tissues were collected between Jan.2003 and Dec.2005.Among them,37 were males and 21 were females with mean age of 58.2 years(ranged from 31 to 76 years).Fifty-eight paracancerous tissues(5-6 cm apart form the cancer)were taken as controls.The expressions of COX-2,MMP-9 and Ki67 in cancerous or paracancerous tissues were examined by immunohistochemistry.Results The expressions of COX-2 and MMP-9 in cancerous tissues(82.76%and 68.9%,respectively)were higher than those in controls(37.93%and 24.14%,respectively,P＜0.01).The expressions of COX-2 and MMP-9 were not associated with sex,age,location and size of gastric cancer(P＞0.05),but were correlated with the depth of lymph node infiltration.metastasis and TNM stage(P＜0.05).The expression of MMP-9 was also associated with the degree of differentiation of gastric cancer(P＜0.05).There was a correlation between COX-2 and MMp-9 expressions in gastric cancer tissues(P＜0.05,C=0.359).The expression of Ki67 in tissues with positive expressions of COX-2 and MMp-9 was higher than that in negative ones(P＜0.O 1).Conclusion The COX-2,MMP-9 and Ki67 play important roles in metastasis and infiltration,which promote occurrence and development of gastric cancer.