CAJ | 학술논문

研究了以霍乱毒素B亚基（CTB）为载体的重组疟疾多价抗原（AWTE）表位的DNA疫苗在恒河猴中的免疫原性及对相应疟原虫感染的免疫保护作用。结果表明：DNA疫苗组免疫2次后即产生了较高水平的细胞免疫和体液免疫，免疫后91天用1.25×108个食蟹疟原虫攻击，对照组5只动物在攻击后14天左右全部感染，感染持续34天以上；DNA疫苗组的5只动物一直到攻击后60天，没有感染。另外，还检测了免疫后不同时间各组的免疫应答水平，与对照组相比，DNA疫苗组免疫2次后即产生了较高水平的细胞免疫和体液免疫。从实验结果来看，首先说明了选择的这种鸡尾酒式的抗原表位组合构建的DNA疫苗具有很好的免疫原性，同时也说明了DNA疫苗在抗疟感染中起着举足轻重的作用。
연구료이곽란독소B아기（CTB）위재체적중조학질다개항원（AWTE）표위적DNA역묘재항하후중적면역원성급대상응학원충감염적면역보호작용。결과표명：DNA역묘조면역2차후즉산생료교고수평적세포면역화체액면역，면역후91천용1.25×108개식해학원충공격，대조조5지동물재공격후14천좌우전부감염，감염지속34천이상；DNA역묘조적5지동물일직도공격후60천，몰유감염。령외，환검측료면역후불동시간각조적면역응답수평，여대조조상비，DNA역묘조면역2차후즉산생료교고수평적세포면역화체액면역。종실험결과래간，수선설명료선택적저충계미주식적항원표위조합구건적DNA역묘구유흔호적면역원성，동시야설명료DNA역묘재항학감염중기착거족경중적작용。
The immunogenicity and protective efficacy of the DNA vaccine which include cholera toxin B subunit (CTB) and polyvalent protective epitopes of Plasmodium falciparum (awte gene) was assessed using rhesus monkeys as animal models. Recombinant plasmids of pCMV-CTB-AWTE were given to five rhesus monkeys three times with two weeks intervals by intramuscle (i.m) route, immunization dose was 500g per plasmid per animal. High levels of anti-CTB and anti-malaria epitopes antibodies and P. falciparum epitope specific CTL activity were elicited. The vaccinated groups was challenged with 1.25×108 of P. cynomolgi parasites. All monkeys of the control group was patent for at least 34 days, the DNA vaccinated groups wasn't infected during the 60 days we detected. The cocktail DNA vaccine which contains multi-stage and multi-epitope antigen gene shows excellent immunogenicity and protective efficacy, the results also suggests that DNA vaccine plays an important role against malaria infection.