CAJ | 학술논문

分析了搭载于实践八号育种卫星的啤酒酵母的存活情况和糖代谢相关酶类活性.啤酒酵母于YPD液体培养基培养,培养过夜后用新鲜YPD稀释10~(-6)倍,分装后分剐置于地面和卫星搭栽两种条件下15 d.返回地面后收集样品,利用稀释平板计数法检测啤酒酵母活力,采用酶解结合分光光度法检测酵母糖原水平,分光光度法检测己糖激酶、琥珀酸脱氢酶和苹果酸脱氢酶的活性.结果发现,卫星搭载样品的菌落形成数显著高于地面对照组,卫星搭载样品是地面对照的3.1倍;卫星搭载啤酒酵母样品的己糖激酶和琥珀酸脱氢酶活性均明显低于地面对照组,而卫星搭载样品的苹果酸脱氢酶活性明显高于地面对照组;卫星搭载样品的糖原水平均低于相对应的地面对照组.表明,太空飞行下可导致啤酒酵母的存活率提高,同时伴有糖代谢相关酶活性和糖原水平的变化,提示太空飞行条件下引起糖代谢变化有利于啤酒酵母存活.
분석료탑재우실천팔호육충위성적비주효모적존활정황화당대사상관매류활성.비주효모우YPD액체배양기배양,배양과야후용신선YPD희석10~(-6)배,분장후분과치우지면화위성탑재량충조건하15 d.반회지면후수집양품,이용희석평판계수법검측비주효모활력,채용매해결합분광광도법검측효모당원수평,분광광도법검측기당격매、호박산탈경매화평과산탈경매적활성.결과발현,위성탑재양품적균락형성수현저고우지면대조조,위성탑재양품시지면대조적3.1배;위성탑재비주효모양품적기당격매화호박산탈경매활성균명현저우지면대조조,이위성탑재양품적평과산탈경매활성명현고우지면대조조;위성탑재양품적당원수평균저우상대응적지면대조조.표명,태공비행하가도치비주효모적존활솔제고,동시반유당대사상관매활성화당원수평적변화,제시태공비행조건하인기당대사변화유리우비주효모존활.
The viable conditions and activities of carbohydrate metabolism related enzymes (CMRE) of Saccharomy-ces cerevisiae took a flight in Practice 8 recoverable satellite for 15 days (space yeast) were analyzed. The yeast was cultured in liquid YPD medium overnight, then diluted with fresh YPD to 10~(-6) times before taking the satellite flight and filled another one to keep on the ground for 15 days as a control. After recovery, the cell viability was determines by colony counting on fiat plate, and the activities of CMRE including hexokinase, suceinate dehydrogenase, malate dehydrogenase, and the content of glycogen in whole cells of both yeasts (satellite and ground) were tested and deter-mined with zymolysis combined with spectrophotometry. The results showed that the colony forming amount of space yeast samples was obviously higher than that of the ground control samples, as high as 3.1 times. However, the activ-ities of hexokinase and snccinate dehydrogenase of space yeast were obviously lower than the ground control one; but the activity of malate dehydrogenase of space yeast was higher than the ground control one. The content of glycogen of space yeast was lower than the ground control one. These indicated that under the space flight could increase the via-bility of the yeast, and at the same time it accompanied with the changes of the activities of related enzymes and the level of glycogen that conduced to the viability of the yeast in space flight conditions.