中国小儿急救医学
中國小兒急救醫學
중국소인급구의학
CHINESE PEDIATRIC EMERGENCY MEDICINE
2010年
1期
37-39
,共3页
郑飞霞%李光乾%施旭来%林忠东
鄭飛霞%李光乾%施旭來%林忠東
정비하%리광건%시욱래%림충동
惊厥,发热性%白细胞介素-1β%白细胞介素-1受体拮拉剂%海马%大鼠
驚厥,髮熱性%白細胞介素-1β%白細胞介素-1受體拮拉劑%海馬%大鼠
량궐,발열성%백세포개소-1β%백세포개소-1수체길랍제%해마%대서
Seizures,febrile%IL-1β%IL-1ra%Hippocampus%Rat
目的 探讨大鼠反复热性惊厥(FS)后海马白细胞介素-1β(IL-1β)和白细胞介素-1受体拈抗剂(IL-1ra)表达的变化.方法 清洁级21日龄雄性SD大鼠随机分为正常对照组(NC组)、高热对照组(HC组)和热性惊厥组(FS组).末次热水浴后12 h处死动物,用ELISA法检测海马IL-1β和IL-1ra的含量;RT-PCR法测海马IL-1β和IL-1ra mRNA的表达水平.结果 Fs组大鼠海马IL-1β蛋白和IL-1β mRNA水平均明显高于NC组和HC组,差异均具有显著性(P＜0.01和P＜0.05);FS组大鼠海马IL-1ra蛋白及IL-1ra mRNA水平亦明显高于HC组和NC组,差异均具有显著性(P＜0.01和P＜0.05).各组大鼠海马IL-1ra/IL-1β蛋白比值差异无显著性(P＞0.05).结论 短期内反复FS可导致海马IL-1β和IL-1ra表达增加,提示IL-1β和IL-1ra可能参与FS脑损伤发病的病理过程.
目的 探討大鼠反複熱性驚厥(FS)後海馬白細胞介素-1β(IL-1β)和白細胞介素-1受體拈抗劑(IL-1ra)錶達的變化.方法 清潔級21日齡雄性SD大鼠隨機分為正常對照組(NC組)、高熱對照組(HC組)和熱性驚厥組(FS組).末次熱水浴後12 h處死動物,用ELISA法檢測海馬IL-1β和IL-1ra的含量;RT-PCR法測海馬IL-1β和IL-1ra mRNA的錶達水平.結果 Fs組大鼠海馬IL-1β蛋白和IL-1β mRNA水平均明顯高于NC組和HC組,差異均具有顯著性(P＜0.01和P＜0.05);FS組大鼠海馬IL-1ra蛋白及IL-1ra mRNA水平亦明顯高于HC組和NC組,差異均具有顯著性(P＜0.01和P＜0.05).各組大鼠海馬IL-1ra/IL-1β蛋白比值差異無顯著性(P＞0.05).結論 短期內反複FS可導緻海馬IL-1β和IL-1ra錶達增加,提示IL-1β和IL-1ra可能參與FS腦損傷髮病的病理過程.
목적 탐토대서반복열성량궐(FS)후해마백세포개소-1β(IL-1β)화백세포개소-1수체념항제(IL-1ra)표체적변화.방법 청길급21일령웅성SD대서수궤분위정상대조조(NC조)、고열대조조(HC조)화열성량궐조(FS조).말차열수욕후12 h처사동물,용ELISA법검측해마IL-1β화IL-1ra적함량;RT-PCR법측해마IL-1β화IL-1ra mRNA적표체수평.결과 Fs조대서해마IL-1β단백화IL-1β mRNA수평균명현고우NC조화HC조,차이균구유현저성(P＜0.01화P＜0.05);FS조대서해마IL-1ra단백급IL-1ra mRNA수평역명현고우HC조화NC조,차이균구유현저성(P＜0.01화P＜0.05).각조대서해마IL-1ra/IL-1β단백비치차이무현저성(P＞0.05).결론 단기내반복FS가도치해마IL-1β화IL-1ra표체증가,제시IL-1β화IL-1ra가능삼여FS뇌손상발병적병리과정.
Objective To evaluate the changes of IL-1β and IL-lra in hippocampus of rats with frequent febrile seizures(FS).Methods 21-day-old male Sprague-Dawley(SD)rats were randomly divided into 3 groups:normal control group(NC group),hyperthermic control group(HC group)and febrile seizures group(FS group).IL-1β and IL-1ra levels in hippocampus of these rats were determined by ELISA methods,IL-1β mRNA and IL-1ra mRNA levels in hippocampus were measured by RT-PCR.Results IL-1β mRNA and protein levels in hippocampus of rats in FS group were significantly higher than those in NC group and HC group(P＜0.01,P＜0.05).IL-1ra mRNA and protein levels in hippocampus of rats in FS group were significantly higher than those in NC group and HC group(P＜0.01,P＜0.05),too.IL-1ra/IL-1β protein ratio showed no significant difference among 3 groups.Conclusion Frequent FS can cause the increase of IL-1β and IL-lra mRNA and protein levels in hippocampus,suggesting that IL-1β and IL-1ra may be involved in the pathogenesis of hippocampal neuron damage in FS rat.
