中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2010年
6期
535-538
,共4页
张英%于风旭%付勇%廖斌%石应康%陈怀卿
張英%于風旭%付勇%廖斌%石應康%陳懷卿
장영%우풍욱%부용%료빈%석응강%진부경
剪切力%凝集素样氧化低密度脂蛋白受体1%动脉粥样硬化%脐静脉内皮细胞
剪切力%凝集素樣氧化低密度脂蛋白受體1%動脈粥樣硬化%臍靜脈內皮細胞
전절력%응집소양양화저밀도지단백수체1%동맥죽양경화%제정맥내피세포
Shear stress%Lectin-like oxidized low-density lipoprotein receptor-1%Atherosclerosis%Umbilical vein endothelial cell
目的 研究不同剪切力对内皮细胞表面凝集素样氧化低密度脂蛋白受体1(LOX-1)表达的影响.方法 体外培养人脐静脉内皮细胞,将其种植于载玻片上,放入流室系统分别施加以不同的剪切力.按施加剪切力的不同分为实验组和对照组:实验组分为低剪切力组(4.2 dyne/cm2,n=5)、中等剪切力组(8.4 dyne/cm2,n=5)和高剪切力组(15.0 dyne/cm2,n=5);对照组(n=5)静态条件下培养,不施加剪切力.剪切力加载1、2、4、8 h后RT-PCR测定各组内皮细胞LOX-1 mRNA的表达.结果 低剪切力(4.2 dyne/cm2)作用下,内皮细胞LOX-1表达随时间延长而增加.与对照组比较,中等剪切力(8.4dyne/cm2)作用2、4、8 h内皮细胞LOX-1的表达水平均降低[2 h:0.2346±0.0397比0.2948±0.0128,4 h:0.1747±0.0585比0.2985±0.0204,8 h:0.0256±0.0067比0.2968±0.0175,均P＜0.05].高剪切力(15.0dyne/cm2)作用下,血管内皮细胞LOX-1表达一直维持在极低水平.结论 不同剪切力可以影响内皮细胞的LOX-1表达水平.
目的 研究不同剪切力對內皮細胞錶麵凝集素樣氧化低密度脂蛋白受體1(LOX-1)錶達的影響.方法 體外培養人臍靜脈內皮細胞,將其種植于載玻片上,放入流室繫統分彆施加以不同的剪切力.按施加剪切力的不同分為實驗組和對照組:實驗組分為低剪切力組(4.2 dyne/cm2,n=5)、中等剪切力組(8.4 dyne/cm2,n=5)和高剪切力組(15.0 dyne/cm2,n=5);對照組(n=5)靜態條件下培養,不施加剪切力.剪切力加載1、2、4、8 h後RT-PCR測定各組內皮細胞LOX-1 mRNA的錶達.結果 低剪切力(4.2 dyne/cm2)作用下,內皮細胞LOX-1錶達隨時間延長而增加.與對照組比較,中等剪切力(8.4dyne/cm2)作用2、4、8 h內皮細胞LOX-1的錶達水平均降低[2 h:0.2346±0.0397比0.2948±0.0128,4 h:0.1747±0.0585比0.2985±0.0204,8 h:0.0256±0.0067比0.2968±0.0175,均P＜0.05].高剪切力(15.0dyne/cm2)作用下,血管內皮細胞LOX-1錶達一直維持在極低水平.結論 不同剪切力可以影響內皮細胞的LOX-1錶達水平.
목적 연구불동전절력대내피세포표면응집소양양화저밀도지단백수체1(LOX-1)표체적영향.방법 체외배양인제정맥내피세포,장기충식우재파편상,방입류실계통분별시가이불동적전절력.안시가전절력적불동분위실험조화대조조:실험조분위저전절력조(4.2 dyne/cm2,n=5)、중등전절력조(8.4 dyne/cm2,n=5)화고전절력조(15.0 dyne/cm2,n=5);대조조(n=5)정태조건하배양,불시가전절력.전절력가재1、2、4、8 h후RT-PCR측정각조내피세포LOX-1 mRNA적표체.결과 저전절력(4.2 dyne/cm2)작용하,내피세포LOX-1표체수시간연장이증가.여대조조비교,중등전절력(8.4dyne/cm2)작용2、4、8 h내피세포LOX-1적표체수평균강저[2 h:0.2346±0.0397비0.2948±0.0128,4 h:0.1747±0.0585비0.2985±0.0204,8 h:0.0256±0.0067비0.2968±0.0175,균P＜0.05].고전절력(15.0dyne/cm2)작용하,혈관내피세포LOX-1표체일직유지재겁저수평.결론 불동전절력가이영향내피세포적LOX-1표체수평.
Objective To investigate the effects of different shear stress on expression of lectinlike oxidized low-density lipoprotein receptor-1 (LOX-1 ) on endothelial cell surface. Methods The human umbilical vein endothelial cells (HUVECs) were cultured in vitro and implanted on microscopic slides which were then put into a flow chamber system and treated with different shear stress. According to different shear stress, HUVECs were allocated into control group (0 dyne/cm2, n=5) and experimental groups including low shear stress group (4.2 dyne/cm2, n=5), moderate shear stress group (8.4 dyne/cm2, n=5) and high shear stress group ( 15.0 dyne/cm2, n=5). The mRNA expression of LOX- 1 of the endothelial cells was detected in all groups with RT-PCR at 1 h, 2 h, 4 h and 8 h after loading of shear stress. Results The expression of LOX- 1 increased over time under low shear stress (4.2 dyne/cm2). Compared with the control group, the expressions of LOX- 1 in endothelial cells were declined at 2 h, 4 h and 8 h after treatment with moderate shear stress (8.4 dyne/cm2) [2 h: 0.2346 ± 0.0397 vs 0.2948 ± 0.0128; 4 h: 0.1747 ± 0.0585 vs 0.2985 ±0.0204; 8 h: 0.0256±0.0067 vs 0.2968±0.0175, all P＜0.05]. However, the expression of LOX- 1 maintained a very low level under high shear stress (15.0 dyne/cm2). Conclusion Different shear stress may have varied effects on the expression of LOX-1 in endothelial cells.
