中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2009年
2期
151-154
,共4页
侯殿俊%马娅%刘伟%乔建维%卢峰%商希梅%李洁清%封丽
侯殿俊%馬婭%劉偉%喬建維%盧峰%商希梅%李潔清%封麗
후전준%마아%류위%교건유%로봉%상희매%리길청%봉려
辐射照射%基因突变%TCR基因%生物剂量计
輻射照射%基因突變%TCR基因%生物劑量計
복사조사%기인돌변%TCR기인%생물제량계
Radiation exposure%Gene mutation%TCR gene%Biological dosimeter
目的 用培养法研究X射线诱发的人外周血淋巴细胞TCR基因突变情况.方法 以不同剂量(0～8 Gy)的X射线照射新鲜分离的健康成人外周血淋巴细胞,植物血凝素、白细胞介素2(IL-2)协同刺激培养7 d,流式细胞术检测TCR基因突变频率(TCR MF),并拟合剂量效应关系.结果 随着照射剂量的增加,TCR基因突变频率随之上升,最佳拟合曲线为二次多项式模型.结论 TCR基因突变可作为辐射生物剂量计,用于急性辐射照射生物剂量的估算.
目的 用培養法研究X射線誘髮的人外週血淋巴細胞TCR基因突變情況.方法 以不同劑量(0～8 Gy)的X射線照射新鮮分離的健康成人外週血淋巴細胞,植物血凝素、白細胞介素2(IL-2)協同刺激培養7 d,流式細胞術檢測TCR基因突變頻率(TCR MF),併擬閤劑量效應關繫.結果 隨著照射劑量的增加,TCR基因突變頻率隨之上升,最佳擬閤麯線為二次多項式模型.結論 TCR基因突變可作為輻射生物劑量計,用于急性輻射照射生物劑量的估算.
목적 용배양법연구X사선유발적인외주혈림파세포TCR기인돌변정황.방법 이불동제량(0～8 Gy)적X사선조사신선분리적건강성인외주혈림파세포,식물혈응소、백세포개소2(IL-2)협동자격배양7 d,류식세포술검측TCR기인돌변빈솔(TCR MF),병의합제량효응관계.결과 수착조사제량적증가,TCR기인돌변빈솔수지상승,최가의합곡선위이차다항식모형.결론 TCR기인돌변가작위복사생물제량계,용우급성복사조사생물제량적고산.
objective To study the TCR gene mutation in peripheral blood lymphocytes induced by X-ray exposure using cultured lymphocytes cloning method.Methods Freshly isolated peripheral lymphocytes from healthy aduh donors were irradiated with X-ray in doses ranging from 0 to 8 Gy and cultured with interleukin2 and phytohemagglutinin for 7 days.The mutant frequencies of TCR gene(TCR MF)were detected by flow cytonletry and the dose response curves were fitted.Results TCR MF increased with the dose going up.An aquadratic polynomial dose response model was fitted.Conclusions TCR gene mutation could which serve as a potential biological dosimeter.It might be applied for the estimation of biological dose in emergency exposure.
