CAJ | 학술논문

目的研究腺病毒介导的BcI-XL shRNA对结肠癌细胞株的体外抗肿瘤性.方法将已有的Bcl-xl腺病毒进行扩增纯化,并通过反转录反应分析该腺病毒在mRNA水平对Bcl-XL的表达调控.通过MTT检测及克隆形成实验,了解该腺病毒对结直肠癌细胞株(Lovo细胞株)的体外杀伤作用.结果 Ad/Bcl-XL shRNA作用于Lovo细胞株导致Bcl-XL mRNA表达水平下降.与对照腺病毒Ad/GFP相比,Ad/Bcl-XL shRNA显著抑制Lovo细胞的增长(P＜0.05),且该抑制效应呈剂量和时间依赖性；显著抑制Lovo细胞的克隆形成(P＜0.05),且该抑制效应呈剂量依赖性.而Ad/Bcl-XL shRNA对正常人成纤维细胞(NHFB)无明显杀伤作用.结论腺病毒介导的Bcl-XL shRNA对Lovo细胞株具有杀伤作用,可能存在潜在的结肠癌治疗价值.
목적 연구선병독개도적BcI-XL shRNA대결장암세포주적체외항종류성.방법 장이유적Bcl-xl선병독진행확증순화,병통과반전록반응분석해선병독재mRNA수평대Bcl-XL적표체조공.통과MTT검측급극륭형성실험,료해해선병독대결직장암세포주(Lovo세포주)적체외살상작용.결과 Ad/Bcl-XL shRNA작용우Lovo세포주도치Bcl-XL mRNA표체수평하강.여대조선병독Ad/GFP상비,Ad/Bcl-XL shRNA현저억제Lovo세포적증장(P＜0.05),차해억제효응정제량화시간의뢰성；현저억제Lovo세포적극륭형성(P＜0.05),차해억제효응정제량의뢰성.이Ad/Bcl-XL shRNA대정상인성섬유세포(NHFB)무명현살상작용.결론 선병독개도적Bcl-XL shRNA대Lovo세포주구유살상작용,가능존재잠재적결장암치료개치.
Objective To investigate the anti-tumor effect of adenovirus-mediated Bcl-XL shRNA on colon cancer cells in vitro.Methods A recombinant Bcl-xl adenovirus was constructed,applified,and purified.The effect on mRNA expression of Bcl-XL was assessed by RT-PCR,and the effect on apoptosis-induction of colon cancer (Lovo cell line) in vitro was assessed by MTT assay and cell clonogenic assay.Results RT-PCR showed that Ad/Bcl-XL shRNA significantly down-regulated the mRNA expression of Bcl-XL in Lovo cells.Ad/Bcl-XL shRNA suppressed the proliferation of Lovo cells in a dose-dependent as well as a time-dependent manner compared with Ad/GFP(P＜0.05).Treatment with Ad/Bcl-XL shRNA dramatically suppressed the colony formation of Lovo cells in a dosedependent manner (P＜0.05).Ad/Bcl-XL shRNA showed no effect on normal human fibroblast.Conclusion Ad/Bcl-XL shRNA exhibits cytotoxic effect on Lovo cells and may have the potential value in the treatment of colon cancer.