CAJ | 학술논문

目的观察胰淀素对大鼠胰岛β细胞电压门控L-型钙通道的影响.方法用全细胞膜片钳技术观察胰淀素作用前后胰岛β细胞电压门控L-型钙通道门控特性、电流变化.结果在葡萄糖5.5 mmol/L条件下,大鼠胰岛β细胞L-型钙通道电流约在-40 mV激活,+20 mV左右达高峰,峰值约为-120 pA,胰岛素分泌量为(0.76±0.12)μg/L;16.7 mmol/L葡萄糖刺激下峰电流电压左移、峰电流达-140 pA左右,胰岛素分泌量为(1.78±0.13)μg/L.用0.5、1.0、5.0、10.0μmol/L浓度胰淀素分别孵育胰岛β细胞,在5.5、16.7 mmoL/L葡萄糖条件下,0.5、1.0μmol/L胰淀素浓度的峰电位激活电压、电流、胰岛素分泌最与对照组相比,差异均无统计学意义,在5.5 mol/L葡萄糖条件下,5.0、10.0μmol/L胰淀素浓度的激活电雎增加为-30 mV,峰电流分别减小至-80 pA、-60 pA左右,胰岛素分泌量分别降低至(0.49±0.11)、(0.36±0.12)μg/L;在16.7 mmol/L葡萄糖条件下,激活电压由-40 mV增加到-30 mV,峰电流分别减小至-80 pA、-40 pA,胰岛素分泌量分别降低至(1.20±0.13)、(0.89±0.14)μg/L,与对照组相比差异具有统计学意义.结论葡萄糖刺激胰岛β细胞电压门控L-型钙通道开放与胰岛素分泌同步,胰淀素抑制胰岛β细胞电压门控L-型钙通道开放和抑制胰岛素分泌作用同步并与其作用浓度正相关.
목적 관찰이정소대대서이도β세포전압문공L-형개통도적영향.방법 용전세포막편겸기술관찰이정소작용전후이도β세포전압문공L-형개통도문공특성、전류변화.결과 재포도당5.5 mmol/L조건하,대서이도β세포L-형개통도전류약재-40 mV격활,+20 mV좌우체고봉,봉치약위-120 pA,이도소분비량위(0.76±0.12)μg/L;16.7 mmol/L포도당자격하봉전류전압좌이、봉전류체-140 pA좌우,이도소분비량위(1.78±0.13)μg/L.용0.5、1.0、5.0、10.0μmol/L농도이정소분별부육이도β세포,재5.5、16.7 mmoL/L포도당조건하,0.5、1.0μmol/L이정소농도적봉전위격활전압、전류、이도소분비최여대조조상비,차이균무통계학의의,재5.5 mol/L포도당조건하,5.0、10.0μmol/L이정소농도적격활전저증가위-30 mV,봉전류분별감소지-80 pA、-60 pA좌우,이도소분비량분별강저지(0.49±0.11)、(0.36±0.12)μg/L;재16.7 mmol/L포도당조건하,격활전압유-40 mV증가도-30 mV,봉전류분별감소지-80 pA、-40 pA,이도소분비량분별강저지(1.20±0.13)、(0.89±0.14)μg/L,여대조조상비차이구유통계학의의.결론 포도당자격이도β세포전압문공L-형개통도개방여이도소분비동보,이정소억제이도β세포전압문공L-형개통도개방화억제이도소분비작용동보병여기작용농도정상관.
Objective To observe the effect of amylin on the islet β-cells voltage-gated L-calcium channels in rats. Method Patch clamp technique was employed in the observation of the features and changes of electric current of islet β-cells voltage-gated L-calcium channels before and after using amylin. Results In the glucose environment of 5. 5 mmoL/L, the electric current of rat islet β-cells voltage-gated L-calcium channels was activated at-40 mV and reached the peak at about +20 mV, with a peak value of about-120 pA and the insulin secretion level was(0. 76±0. 12) μg/L. Under the stimulation of glucose of 16. 7 mmol/L, the peak current voltage moved to the left and increased up to-140 pA and the level of insulin secretion measured (1.78±0. 13) μg/L. Hatch islet β-cells in amylin at the concentrations of 0. 5, 1.0, 5.0 and 10.0 μmol/L, respectively. It was observed that in the 0. 5 μmol/L and 1.0 μmol/L groups,there was no remarkable change in the peak potential activation voltage, current, and insulin secretion volume in comparison with the control group. However, in the environment of 5.5 mmol/L glucose, the increase of activation voltage of the 5.0 and 10.0 μmol/L groups was-30 mV, with the peak current reduced to approximately-80 pA and-60 pA and the insulin secretion decreased to (0. 49±0. 11) μg/L and (0. 36±0. 12) μg/L respectively. Under the concentration of 16. 7 mmol/L glucose, the activation voltage increased from-40 mV up to-30 mV and the peak current reduced to-80 pA and-40 pA. In the meantime, the insulin secretion decreased respectively to (1.20±0. 13) μg/L and (0. 89±0. 14) μg/L, which is of significance. Conclusion The secretion of insulin is synchronized with the opening of the islet β-cells voltage-gated L-calcium channels at the stimulation of glucose. The amylin inhibition of the insulin secretion is also synchronized with the opening of islet β-cells voltage-gated L-calcium channels and it's in a positive concentration-dependent manner.