中华医学遗传学杂志
中華醫學遺傳學雜誌
중화의학유전학잡지
CHINESE JOURNAL OF MEDICAL GENETICS
2010年
2期
217-220
,共4页
戴卫健%朱发明%严力行%赵洪灿
戴衛健%硃髮明%嚴力行%趙洪燦
대위건%주발명%엄역행%조홍찬
人类白细胞抗原DPA1%人类白细胞抗原DPB1%遗传多态性%基因频率%单倍型
人類白細胞抗原DPA1%人類白細胞抗原DPB1%遺傳多態性%基因頻率%單倍型
인류백세포항원DPA1%인류백세포항원DPB1%유전다태성%기인빈솔%단배형
human leukocyte antigen-DPA1%human leukocyte antigen-DPB1%genetic polymorphism%gene frequency%haplotype
目的 检测浙江地区汉族人群HLA-DPA1和HLA-DPB1等位基因及单倍型频率.方法 应用PCR-直接测序分型法对100名健康、无血缘关系的浙江汉族人外周血标本进行HLA-DPA1和HLADPB1等位基因分析.结果 在100份标本中检出8个HLA-DPA1等位基因和19个HLA-DPB1等位基因.HLA-DPA1等位基因频率较高的依次为DPA1*020202(47.0%)、DPA1*010301(38.5%)和DPA1*020101(10.5%).HLA-DPB1等位基因中,频率较高的依次为DPB1*0501(39.5%)、DPB1*020102(13.5%)和DPB1*040101(13.0%).连锁分析发现共有44个HLA-DPA1-DPB1单倍型,单倍型频率最高为DPA1*020202-DPB1*0501(29.5%).结论 浙江地区汉族人群HLA-DPA1和DPB1基因座等位基因具有丰富的多态性,2个基因座呈现连锁不平衡.
目的 檢測浙江地區漢族人群HLA-DPA1和HLA-DPB1等位基因及單倍型頻率.方法 應用PCR-直接測序分型法對100名健康、無血緣關繫的浙江漢族人外週血標本進行HLA-DPA1和HLADPB1等位基因分析.結果 在100份標本中檢齣8箇HLA-DPA1等位基因和19箇HLA-DPB1等位基因.HLA-DPA1等位基因頻率較高的依次為DPA1*020202(47.0%)、DPA1*010301(38.5%)和DPA1*020101(10.5%).HLA-DPB1等位基因中,頻率較高的依次為DPB1*0501(39.5%)、DPB1*020102(13.5%)和DPB1*040101(13.0%).連鎖分析髮現共有44箇HLA-DPA1-DPB1單倍型,單倍型頻率最高為DPA1*020202-DPB1*0501(29.5%).結論 浙江地區漢族人群HLA-DPA1和DPB1基因座等位基因具有豐富的多態性,2箇基因座呈現連鎖不平衡.
목적 검측절강지구한족인군HLA-DPA1화HLA-DPB1등위기인급단배형빈솔.방법 응용PCR-직접측서분형법대100명건강、무혈연관계적절강한족인외주혈표본진행HLA-DPA1화HLADPB1등위기인분석.결과 재100빈표본중검출8개HLA-DPA1등위기인화19개HLA-DPB1등위기인.HLA-DPA1등위기인빈솔교고적의차위DPA1*020202(47.0%)、DPA1*010301(38.5%)화DPA1*020101(10.5%).HLA-DPB1등위기인중,빈솔교고적의차위DPB1*0501(39.5%)、DPB1*020102(13.5%)화DPB1*040101(13.0%).련쇄분석발현공유44개HLA-DPA1-DPB1단배형,단배형빈솔최고위DPA1*020202-DPB1*0501(29.5%).결론 절강지구한족인군HLA-DPA1화DPB1기인좌등위기인구유봉부적다태성,2개기인좌정현련쇄불평형.
Objective To investigate the polymorphisms of HLA-DPA1 and HLA-DPB1 loci of Han population in Zhejiang province of China. Methods The alleles of HLA-DPAl and HLA-DPB1 loci in 100 unrelated healthy individuals were analyzed using polymerase chain reaction-sequence based typing. Results Eight HLA-DPA1 alleles and 19 HLA-DPB1 alleles were found in the population. The HLA-DPA1 alleles with higher frequencies were DPA1 * 020202 (47.0%), DPA1 * 010301 (38.5%) and DPA1 *020101(10.5%). The HLA-DPB1 alleles with higher frequencies were DPB1 * 0501, DPB1 * 020102 and DPB1 * 040101. The frequencies were 39.5%, 13.5% and 13.0%, respectively. A total of 44 estimated DPA1-DPB1 haplotypes were detected. The HLA-DPA1 * 020202-DPB1 * 0501 (29.5%) was the most frequent haplotype. Conclusion The polymorphism data of the HLA-DPA1 and-DPB1 were obtained in Han population in Zhejiang province of China. There was linkage disequilibrium between the two loci.
