CAJ | 학술논문

目的研究1年以内感染HIV的感染者(早期感染者,EHI)体内CD+4 CD+25 Foxp3+调节性T淋巴细胞水平及其与疾病进展相关性.方法随机选取51例HIV感染者,依据感染时间及CD+4 T淋巴细胞水平分为3组:EHI组30例、HIV组15例、AIDS组6例,20名健康人作为对照组,各组对象的年龄、性别具有可比性.用EDTA抗凝管采集全血,应用FACSAria流式细胞仪及Foxp3染色试剂盒,检测外周血单个核细胞CD+4CD+25Foxp3+调节性T淋巴细胞表达水平,分析EHI者及全部HIV感染者CD+4 CD+25Foxp3+调节性T淋巴细胞表达水平与CD+4T淋巴细胞数量、病毒调定点、病毒载量及淋巴细胞活化水平间的相关性.结果健康对照组、EHI组、HIV组及AIDS组CD+4C+25Foxp3+T淋巴细胞百分率逐级上升,其中EHI组CD+4CD+25Foxp3+T淋巴细胞百分率[3.79(2.11～5.43)%]低于AIDS组[8.09(4.90～8.90)%],差异有统计学意义(Z=-2.29,P=0.022);EHI组CD+4 CD+25Foxp3+T淋巴细胞百分率与病毒调定点正相关(r=0.479,P=0.038),与CD4T淋巴细胞计数呈负相关(r=-0.455,P=0.011),与CD+3 HLA+T淋巴细胞呈正相关(r=0.533,P=0.002).结论中国EHI者CD+4 CD+25Foxp3+调节性T淋巴细胞百分率高与高病毒调定点及低CD+4 T淋巴细胞数量相关,提示CD+4 CD+25Fox3+调节性T淋巴细胞是加速HIV感染早期疾病进展的因素之一.
목적 연구1년이내감염HIV적감염자(조기감염자,EHI)체내CD+4 CD+25 Foxp3+조절성T림파세포수평급기여질병진전상관성.방법 수궤선취51례HIV감염자,의거감염시간급CD+4 T림파세포수평분위3조:EHI조30례、HIV조15례、AIDS조6례,20명건강인작위대조조,각조대상적년령、성별구유가비성.용EDTA항응관채집전혈,응용FACSAria류식세포의급Foxp3염색시제합,검측외주혈단개핵세포CD+4CD+25Foxp3+조절성T림파세포표체수평,분석EHI자급전부HIV감염자CD+4 CD+25Foxp3+조절성T림파세포표체수평여CD+4T림파세포수량、병독조정점、병독재량급림파세포활화수평간적상관성.결과 건강대조조、EHI조、HIV조급AIDS조CD+4C+25Foxp3+T림파세포백분솔축급상승,기중EHI조CD+4CD+25Foxp3+T림파세포백분솔[3.79(2.11～5.43)%]저우AIDS조[8.09(4.90～8.90)%],차이유통계학의의(Z=-2.29,P=0.022);EHI조CD+4 CD+25Foxp3+T림파세포백분솔여병독조정점정상관(r=0.479,P=0.038),여CD4T림파세포계수정부상관(r=-0.455,P=0.011),여CD+3 HLA+T림파세포정정상관(r=0.533,P=0.002).결론 중국EHI자CD+4 CD+25Foxp3+조절성T림파세포백분솔고여고병독조정점급저CD+4 T림파세포수량상관,제시CD+4 CD+25Fox3+조절성T림파세포시가속HIV감염조기질병진전적인소지일.
Objective To study the alternations of regulatory T cells in early HIV infected patients and its association with disease progression.Methods Fifty-one untreated HIV infected patients were enrolled and divided into 3 groups according to their infection time and CD+4 T cell levels(30 early HIV infected patients,15 typical progressors,6 AIDS patients).Twenty normal controls were enrolled.There were no significant differences between the age and sex among four groups.Blood was drawn by venipuncture from each subject in EDTA tubes and the levels of CD+4 CD+25 Foxp3 + regulatory T cells were detected by FACSAria flow-cytometry.Spearman correlation was used to detect association between CD+4 CD+25 Foxp3 + regulatory T cells and the absolute CD+4 T cells,viral load and activation of T cells.Results The levels of CD+4 CD+25Foxp3+ regulatory T cells showed the tendency of increasing tendency from normal control to early HIV infected patients,asymptomatic HIV infected patients and AIDS patients.Early HIV infected patients was significantly lower than that in AIDS group [3.79(2.11 - 5.43) % vs 8.09(4.90 - 8.90) %,Z = - 2.29,P = 0.022].The levels of CD+4 CD+25 Foxp3 + Treg cells were associated with viral set point(r = 0.479,P =0.038) and inversely associated with CD+4 T cells(r = -0.455,P =0.011) and closely associated with HLA-DR expression on CD+3 T cells(r = 0.533,P = 0.002).Conclusions The ratio of CD+4 CD+25 Foxp3 +regulatory T cells of early HIV infected patients was significantly increased and associated with viral set point and CD+4 T cell counts,which indicate that alternation of regulatory T cell may be an important factor contributing to the disease progression in early HIV infection.