CAJ | 학술논문

目的建立针对甲型流感病毒血凝素的通用抗体.方法通过对流感病毒数据库中血凝素基因的分析,寻找保守的序列并合成多肽.将多肽耦联在载体匙孔血监蛋白(KLH)上,多次皮下免疫家兔,制备高效价抗体.结果序列分析发现血凝素第二亚单位(HA2)上氨基端14个氨基酸极端保守,耦联多肽经4次免疫后效价达到1:80000,以免疫印迹方法证明此通用抗体能与流感病毒H1～H13亚型的血凝素蛋白特异反应.结论通过寻找保守蛋白并耦联在合适载体上,免疫家兔获得了针对甲型流感病毒血凝素抗原的通用抗体.
목적 건립침대갑형류감병독혈응소적통용항체.방법 통과대류감병독수거고중혈응소기인적분석,심조보수적서렬병합성다태.장다태우련재재체시공혈감단백(KLH)상,다차피하면역가토,제비고효개항체.결과 서렬분석발현혈응소제이아단위(HA2)상안기단14개안기산겁단보수,우련다태경4차면역후효개체도1:80000,이면역인적방법증명차통용항체능여류감병독H1～H13아형적혈응소단백특이반응.결론 통과심조보수단백병우련재합괄재체상,면역가토획득료침대갑형류감병독혈응소항원적통용항체.
Objective To establish the universal antibody against all subtypes of the influenza A viral hemagglutinins(HA). Methods All HA sequences from influenza viruses in common domains were analyzed to search for the most conservative re,on of the HA gene. The peptides conjugated to carrier proteins were used to immunize rabbits in order to obtain high titer antibody. Results The most conservative region among all influenza viruses HA genes were 14 amino acids located at the N-terminal of HA2. The antibody titer reached to 1 : 80 000 after 4 injections of the coupled peptides. The achieved antibody was demonstrated by Western blot to bind HA proteins of influenza virus subtypes H1-H13 specifically. Conclusion The universal antibody has been successfully established by immunizing the rabbits with most conservative peptides of HA protein coupled to carrier protein.