CAJ | 학술논문

为了解水稻(Oryza sativa L.)对氮饥饿反应的分子与基因背景,利用RaSH策略构建了水稻氮(N)饥饿诱导cDNA文库.通过反向Northern筛选该文库,获得氮饥饿诱导的18个功能已知基因和2个功能未知基因.这些已知基因涉及碳代谢、次生代谢产物合成、蛋白质分解代谢、激素代谢、信号转导、生长调控过程及转录因子.这些基因表现出不同的时空表达模式.研究结果表明了植物对氮饥饿反应涉及互相关联的多种生理与分子机理,提供了相关的一些基因信息.
위료해수도(Oryza sativa L.)대담기아반응적분자여기인배경,이용RaSH책략구건료수도담(N)기아유도cDNA문고.통과반향Northern사선해문고,획득담기아유도적18개공능이지기인화2개공능미지기인.저사이지기인섭급탄대사、차생대사산물합성、단백질분해대사、격소대사、신호전도、생장조공과정급전록인자.저사기인표현출불동적시공표체모식.연구결과표명료식물대담기아반응섭급호상관련적다충생리여분자궤리,제공료상관적일사기인신식.
To understand the regulation system of nitrogen (N)-starvation in higher plants, a cDNA libraryfrom N-starved rice (Oryza sativa L.) seedlings was constructed using rapid subtraction hybridization (RaSH)procedure. Through reverse Northern analysis and Northern blotting, 18 unique known genes and twounique unknown genes were identified, which were up-regulated by N-starvation in rice. The known genesare involved in several metabolisms including carbon metabolism, secondary metabolite synthesis,ubiquitylation and protein degradation, phytohormone metabolism, signal transduction, growth regulatorand transcription factors. Different induced expression patterns based on spatial and temporal express ionswere found for these genes. The results indicate the cross-talks between N-starvation response andvarious metabolisms in plants.