CAJ | 학술논문

目的通过体外细胞学实验观察熊果苷拮抗H2O2的作用.方法体外培养人脐静脉内皮细胞ECV-304,将细胞分为对照组、损伤组和熊果苷组,熊果苷组提前12h加入熊果苷,500μmol/L H2O2诱导细胞氧化应激状态.光镜实验比较各组细胞形态学的变化;MTT实验比较各组细胞增殖活力的改变.结果光镜下可见,对照组细胞结构清晰,呈单层铺路石状紧密排列;损伤组细胞变形、皱缩、排列紊乱、脱落明显,胞浆内出现空泡;熊果苷组细胞形态结构基本清晰,但细胞有轻度肿胀,其它接近于正常对照组;MTT结果显示,与损伤组相比,熊果苷能明显保护ECV-304细胞增殖活力(P<0.05).结论本实验结果提示,熊果苷可以抵御H2O2所致ECV-304细胞氧化应激损伤.
목적통과체외세포학실험관찰웅과감길항H2O2적작용.방법체외배양인제정맥내피세포ECV-304,장세포분위대조조、손상조화웅과감조,웅과감조제전12h가입웅과감,500μmol/L H2O2유도세포양화응격상태.광경실험비교각조세포형태학적변화;MTT실험비교각조세포증식활력적개변.결과광경하가견,대조조세포결구청석,정단층포로석상긴밀배렬;손상조세포변형、추축、배렬문란、탈락명현,포장내출현공포;웅과감조세포형태결구기본청석,단세포유경도종창,기타접근우정상대조조;MTT결과현시,여손상조상비,웅과감능명현보호ECV-304세포증식활력(P<0.05).결론본실험결과제시,웅과감가이저어H2O2소치ECV-304세포양화응격손상.
ObjectiveTo study the protection of arbutin against the damage caused by H2O2 in ECV-304 cells.MethodsThe cells were cultured in vitro and were divided into three groups:control group,damage group and arbutin group in which the cells were pretreated with 500μg/L arbutin, while the same amount of PBS was added to the other two groups. After 12h, the cells in damage group and arbutin group were treated with 500μmol/L H2O2. After 1h, light microscope and MTT were used to observe the change of the cells in three groups.ResultsThrough light microscope, in control group, the cells grew in pebble-shaped arrangement. In damage group, the cells became out of shape and most of them peeled off. In arbutin group, the cells were in a better state which was similar to that of control group except the slight swelling of the cells; MTT assay showed significant difference between damage group and arbutin group (P<0.05). ConclusionArbutin could protect ECV-304 cells from the damage caused by H2O2.