CAJ | 학술논문

目的研究大黄素(1,3,8-三羟基-6-甲基蒽醌)是否能减轻急性坏死性胰腺炎(ANP)大鼠肺组织的炎症反应. 方法通过胰腺被膜下注射3%牛磺胆酸钠制造大鼠急性坏死性胰腺炎模型,大黄素以灌肠的方式给药,检测在急性坏死性胰腺炎给药后3、6和12 h的血浆淀粉酶变化,用半定量RT-PCR方法检测大鼠肺组织IL-1β,IL-6和IL-10的表达,免疫组化方法观察肺内IL-1β转化酶(ICE,又称caspase-1)的改变. 结果在未造模组血浆淀粉酶(1611.20±218.72)IU/L.在造模后3、6和12 h,急性坏死性胰腺炎模型组(未用大黄素治疗组)血浆淀粉酶分别为(1981.40±56.81)IU/L,(3287.40±612.37)IU/L 和(4914.60±746.82)IU/L,与未造模组比较有显著性差异(P＜0.05),在给予大黄素治疗后血浆淀粉酶3、6和12 h 分别为(1617.20±136.80)IU/L,(2323.40±318.19)IU/L 和(2670.20±390.03)IU/L.RT-PCR表明给药组与未给药组比较IL-1β和IL-6 mRNA的表达随着时间的改变而降低, IL-10在mRNA上表达增加.免疫组化显示IL-1β转化酶(ICE)在各时间点给药组与未给药组比较其表达减少. 结论大黄素对大鼠急性坏死性胰腺炎肺组织炎症反应有一定的抗炎作用,其抗炎作用的机制可能与大黄素下调IL-1β和IL-6 mRNA的表达和上调IL-10 mRNA的表达有关.
목적 연구대황소(1,3,8-삼간기-6-갑기은곤)시부능감경급성배사성이선염(ANP)대서폐조직적염증반응. 방법 통과이선피막하주사3%우광담산납제조대서급성배사성이선염모형,대황소이관장적방식급약,검측재급성배사성이선염급약후3、6화12 h적혈장정분매변화,용반정량RT-PCR방법검측대서폐조직IL-1β,IL-6화IL-10적표체,면역조화방법관찰폐내IL-1β전화매(ICE,우칭caspase-1)적개변. 결과 재미조모조혈장정분매(1611.20±218.72)IU/L.재조모후3、6화12 h,급성배사성이선염모형조(미용대황소치료조)혈장정분매분별위(1981.40±56.81)IU/L,(3287.40±612.37)IU/L 화(4914.60±746.82)IU/L,여미조모조비교유현저성차이(P＜0.05),재급여대황소치료후혈장정분매3、6화12 h 분별위(1617.20±136.80)IU/L,(2323.40±318.19)IU/L 화(2670.20±390.03)IU/L.RT-PCR표명급약조여미급약조비교IL-1β화IL-6 mRNA적표체수착시간적개변이강저, IL-10재mRNA상표체증가.면역조화현시IL-1β전화매(ICE)재각시간점급약조여미급약조비교기표체감소. 결론 대황소대대서급성배사성이선염폐조직염증반응유일정적항염작용,기항염작용적궤제가능여대황소하조IL-1β화IL-6 mRNA적표체화상조IL-10 mRNA적표체유관.
Objective To investigate whether emodin(1,3,8-trihydroxy-6-methylanthraquinone) can attenuate inflammatory response in rats' lungs with acute necrotic pancreatitis (ANP). Method Acute necrotic pancreatitis model was induced by injection of 3% sodium taurocholic acid into the subcapsular of pancreas and emodin was administered by intestine perfusion. Plasma amylase of 3, 6 and 12 h with acute necrotic pancreatitis was measured together with the detection of IL-1β, IL-6 and IL-10 mRNA expressions in rats' lungs by semi-quantitative RT-PCR. Immunohistochemistry was detected the expression of IL-1β converting enzyme (ICE) in the rats' lungs. Result Plasma amylase of 3, 6 and 12 h with acute necrotic pancreatitis groups are obviously high as compared with normal group(P＜0.05). Plasma amylase was (1 611.20±218.72)IU/L in normal group. Plasma amylase of 3, 6 and 12 h with acute necrotic pancreatitis groups were (1 981.40±56.81)IU/L, (3 287.40±612.37)IU/L and (4 914.60±746.82)IU/L. Plasma amylase of 3, 6 and 12 h with acute necrotic pancreatitis after treatment with emodin groups were obviously low as compared with acute necrotic pancreatitis groups. The plasma amylase was(1 617.20±136.80)IU/L,(2 323.40±318.19) IU/L and (2 670.20±390.03)IU/L respectively. The study showed that the mRNA expression of pro-inflammatory cytokin IL-1β and the expression of IL-6, as well as the expression of IL-1β converting enzyme(ICE) were decreased and IL-10 was increased. Conclusion The study demonstrates that emodin plays an important role in reducing plasma amylase level. Emodin exerts anti-inflammatory effects in acute necrotic pancreatitis rats' lungs by downregulating the mRNA expression of IL-1β and IL-6 and upregulating the mRNA expression of IL-10.