CAJ | 학술논문

为了从环境中筛选高效降解血红蛋白的菌株,采用鲜血琼脂平板,以产生溶血圈比值大小为判定标准,从南京某屠宰场下水道中分离到一株具有较强降解血红蛋白能力的菌株NJM4.经形态学观察、生理生化测定和16S rRNA基因分析(GenBank登陆号为EU234500),初步认为该菌株为短小芽孢杆菌(Bacillus pumilus).该菌株在血红蛋白溶液中可将其逐渐降解为小分子的多肽.在血红蛋白发酵培养基中于37℃、120 r/min培养24 h,降解率达53.64 %.
위료종배경중사선고효강해혈홍단백적균주,채용선혈경지평판,이산생용혈권비치대소위판정표준,종남경모도재장하수도중분리도일주구유교강강해혈홍단백능력적균주NJM4.경형태학관찰、생리생화측정화16S rRNA기인분석(GenBank등륙호위EU234500),초보인위해균주위단소아포간균(Bacillus pumilus).해균주재혈홍단백용액중가장기축점강해위소분자적다태.재혈홍단백발효배양기중우37℃、120 r/min배양24 h,강해솔체53.64 %.
To obtain high efficient degradation bacteria of hemoglobin, strain NJM4 from slaughterhouse sewer in Nanjing was isolated by blood agar plate. The ability of degeneration hemoglobin was assessed by hematolysis circle ratio. Initially, the strain was identified as Bacillus pumiius based on morphology observation, physicochemical characteristics and 16S rRNA sequence analysis (GenBank ID: EU234500). The results showed that the hemoglobin could be degraded into small molecular multi-peptides, and after 24 h incubation under 37 ℃ , 120 r/min, the degradation rate of hemoglobin could reach to 53. 64%.