肿瘤
腫瘤
종류
TUMOR
2010年
4期
322-327
,共6页
乳腺肿瘤%抗原,肿瘤相关,碳水化合物%肿瘤相关糖蛋白72%葡萄糖调节蛋白94%免疫组织化学
乳腺腫瘤%抗原,腫瘤相關,碳水化閤物%腫瘤相關糖蛋白72%葡萄糖調節蛋白94%免疫組織化學
유선종류%항원,종류상관,탄수화합물%종류상관당단백72%포도당조절단백94%면역조직화학
Breast neoplasms%Antigens,tumor-associated,carbohydrate%Tumor-associated glycoprotein 72%Glucose-regulated protein 94%Immunohistochemistry
目的:研究乳腺良恶性病变组织中肿瘤相关糖蛋白72(tumor-associated glycoprotein, TAG72)和葡萄糖调节蛋白94(glucose-regulated protein 94, GRP94)的表达及其临床病理意义.方法:采用免疫组织化学法检测60例乳腺癌组织和30例乳腺良性病变组织中TAG72和GRP94的表达,并进行评分.应用Kaplan-Meier法对乳腺癌患者进行生存分析.结果: 乳腺癌组织中TAG72和GRP94表达的阳性率分别为68.3%和63.3%,其总评分分别为2.53±1.86和2.73±2.23;乳腺良性病变组织的TAG72和GRP94表达阳性率分别为10.0%和13.3%,其总评分分别为0.37±1.13和0.47±1.22;乳腺癌组织与乳腺良性病变组织相比,差异均有统计学意义(P = 0.000).导管内癌、组织学分级为Ⅰ或Ⅱ级、肿瘤最大径≤3 cm、无淋巴结转移、雌激素受体阳性、p53阴性及CA15-3阴性乳腺癌患者的TAG72和GRP94表达阳性率及其评分均明显低于浸润性导管癌(P＜0.05)、组织学分级Ⅲ级(P＜0.01)、肿瘤最大径＞3 cm(P＜0.05)、淋巴结转移(P＜0.01)、雌激素受体阴性(P＜0.05)、p53阳性(P＜0.01)和CA15-3阳性(P＜0.01)的乳腺癌患者.TAG72和GRP94表达阳性的乳腺癌患者及两者均表达阳性的乳腺癌患者的术后生存期明显短于阴性表达者(P＜0.01).COX多因素分析结果显示,TAG74和(或)GRP94表达阳性均与术后生存率呈负相关,与死亡率呈正相关,均是独立的预后因素.结论:TAG72和GRP94表达在乳腺癌的发生、发展、临床生物学行为及预后方面可能具有重要作用,TAG72和(或)GRP94表达阳性的乳腺癌患者预后不良.
目的:研究乳腺良噁性病變組織中腫瘤相關糖蛋白72(tumor-associated glycoprotein, TAG72)和葡萄糖調節蛋白94(glucose-regulated protein 94, GRP94)的錶達及其臨床病理意義.方法:採用免疫組織化學法檢測60例乳腺癌組織和30例乳腺良性病變組織中TAG72和GRP94的錶達,併進行評分.應用Kaplan-Meier法對乳腺癌患者進行生存分析.結果: 乳腺癌組織中TAG72和GRP94錶達的暘性率分彆為68.3%和63.3%,其總評分分彆為2.53±1.86和2.73±2.23;乳腺良性病變組織的TAG72和GRP94錶達暘性率分彆為10.0%和13.3%,其總評分分彆為0.37±1.13和0.47±1.22;乳腺癌組織與乳腺良性病變組織相比,差異均有統計學意義(P = 0.000).導管內癌、組織學分級為Ⅰ或Ⅱ級、腫瘤最大徑≤3 cm、無淋巴結轉移、雌激素受體暘性、p53陰性及CA15-3陰性乳腺癌患者的TAG72和GRP94錶達暘性率及其評分均明顯低于浸潤性導管癌(P＜0.05)、組織學分級Ⅲ級(P＜0.01)、腫瘤最大徑＞3 cm(P＜0.05)、淋巴結轉移(P＜0.01)、雌激素受體陰性(P＜0.05)、p53暘性(P＜0.01)和CA15-3暘性(P＜0.01)的乳腺癌患者.TAG72和GRP94錶達暘性的乳腺癌患者及兩者均錶達暘性的乳腺癌患者的術後生存期明顯短于陰性錶達者(P＜0.01).COX多因素分析結果顯示,TAG74和(或)GRP94錶達暘性均與術後生存率呈負相關,與死亡率呈正相關,均是獨立的預後因素.結論:TAG72和GRP94錶達在乳腺癌的髮生、髮展、臨床生物學行為及預後方麵可能具有重要作用,TAG72和(或)GRP94錶達暘性的乳腺癌患者預後不良.
목적:연구유선량악성병변조직중종류상관당단백72(tumor-associated glycoprotein, TAG72)화포도당조절단백94(glucose-regulated protein 94, GRP94)적표체급기림상병리의의.방법:채용면역조직화학법검측60례유선암조직화30례유선량성병변조직중TAG72화GRP94적표체,병진행평분.응용Kaplan-Meier법대유선암환자진행생존분석.결과: 유선암조직중TAG72화GRP94표체적양성솔분별위68.3%화63.3%,기총평분분별위2.53±1.86화2.73±2.23;유선량성병변조직적TAG72화GRP94표체양성솔분별위10.0%화13.3%,기총평분분별위0.37±1.13화0.47±1.22;유선암조직여유선량성병변조직상비,차이균유통계학의의(P = 0.000).도관내암、조직학분급위Ⅰ혹Ⅱ급、종류최대경≤3 cm、무림파결전이、자격소수체양성、p53음성급CA15-3음성유선암환자적TAG72화GRP94표체양성솔급기평분균명현저우침윤성도관암(P＜0.05)、조직학분급Ⅲ급(P＜0.01)、종류최대경＞3 cm(P＜0.05)、림파결전이(P＜0.01)、자격소수체음성(P＜0.05)、p53양성(P＜0.01)화CA15-3양성(P＜0.01)적유선암환자.TAG72화GRP94표체양성적유선암환자급량자균표체양성적유선암환자적술후생존기명현단우음성표체자(P＜0.01).COX다인소분석결과현시,TAG74화(혹)GRP94표체양성균여술후생존솔정부상관,여사망솔정정상관,균시독립적예후인소.결론:TAG72화GRP94표체재유선암적발생、발전、림상생물학행위급예후방면가능구유중요작용,TAG72화(혹)GRP94표체양성적유선암환자예후불량.
Objective:To study the expression levels of tumor-associated glycoprotein (TAG72) and glucose-regulated protein 94 (GRP94) in the breast benign and malignant lesions and detect their clinicopathological significance. Methods:EnVisionTM immunohistochemistry was used for assaying expressive levels of TAG72 and GRP94 in conventional paraffin-embedded sections from specimens of breast cancer (n=60) and benign lesions (n=30). The survival analysis was performed by using Kaplan-Meier method.Results:The positive rates and scores of TAG72 and GRP94 were significantly higher in breast cancer than those in benign lesions (TAG72: 68.3% vs 10.0%, 2.53±1.86 vs 0.37±1.13; GRP94: 63.3% vs 13.3%, 2.73±2.23 vs 0.47±1.22; P=0.000). The positive rates and scores of TAG72 and GRP94 were significantly lower in the intra-ductal,histological grade Ⅰ or Ⅱ , with maximal diameter ≤3 cm, without lymph node metastasis, estrogen receptor (ER)-positive, p53 and CA15-3-negative breast cancer than those in the ductal invasive (P<0.05), histological grade Ⅲ (P<0.01), maximal diameter >3 cm (P<0.05), with lymph node metastasis (P<0.01), ER-negative (P<0.05) , p53-positive (P<0.01), and CA15-3-positive (P<0.01) breast cancer tissues. Kaplan-Meier survival analysis showed that the median survival time were significantly shorter in the TAG72-positive, GRP94-positive or TAG72 and GRP94-positive breast cancer patients than those with negative TAG72 and/or GRP94 expression (P<0.01). Multivariate COX regression analysis showed that TAG72 and/or GRP94-positive expression were negatively associated with postoperative survival rate and positively related with death rate. Both of them were independent prognostic factors for breast cancer. Conclusion:The expressive levels of TAG72 and/or GRP94 might have important effects on the carcinogenesis, progression, biologic behaviors and prognosis of breast cancer. The breast cancer patients with over-expression of TAG72 and/or GRP94 might have poor prognosis.
