CAJ | 학술논문

[目的]通过推理选育,获得高产的阿维菌素工业生产菌种。[方法]以Biok Av-023为出发菌株,首先采用常规紫外诱变筛选出正突变菌株,再通过添加L-异亮氨酸诱导推理选育方法选育高产阿维菌素生产菌株。[结果]经紫外诱变和L-Ile定向筛选表明,L-Ile筛选浓度以0.5%最佳,经复筛后获得的高产突变菌株AV60s-32最高效价可达4 520 IU/ml,与出发菌株相比提高了23.4%。[结论]采用紫外诱变和L-Ile推理选育相结合的方法可有效提高阿维菌素菌种发酵效价。
[목적]통과추리선육,획득고산적아유균소공업생산균충。[방법]이Biok Av-023위출발균주,수선채용상규자외유변사선출정돌변균주,재통과첨가L-이량안산유도추리선육방법선육고산아유균소생산균주。[결과]경자외유변화L-Ile정향사선표명,L-Ile사선농도이0.5%최가,경복사후획득적고산돌변균주AV60s-32최고효개가체4 520 IU/ml,여출발균주상비제고료23.4%。[결론]채용자외유변화L-Ile추리선육상결합적방법가유효제고아유균소균충발효효개。
[Objective] Strain Biok Av-023 used as the control was employed on screening of high-avermectin yield mutants by rational screening.[Method] With Biok Av-023 as the original strain,the positive mutation strain was firstly screened by routine UV mutagenesis,and then the high-yield avermectin producing strain was selected by the breeding way inferred by L-Ile induction.[Result] UV mutation and L-Ile directional screening had showed that the best L-Ile screening concentration was 0.5%,and the high-yield mutation strain AV60s-32 after re-screening reached the highest titer of 4520 IU/ml,which increased by 23.4% compared with the original strain.[Conclusion] The production of avermectin can be effectively enhanced by the combined way of UV mutation and L-Ile rational breeding.