CAJ | 학술논문

目的评价血管内皮祖细胞(endothelial progenitor cells,EPCs)促血管化组织工程骨修复兔桡骨大段骨缺损的效果.方法选用68只新西兰大耳白兔,以数字随机法分为3组.实验组:EPCs+经成骨诱导的骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)+脱钙骨基质(decalcified bone matrix,DBM);自身对照组:BMSCs+DBM;阴性对照组:单纯DBM.将上述材料置入桡骨中段15 mm骨缺损区,术后12,16周摄X线片行骨密度、组织学光镜、骨钙素免疫组化染色及生物力学测试.结果实验组的骨痂生长、塑形、髓腔再通、骨愈合速度及力学强度等方面均明显优于对照组.结论 EPCs促血管化组织工程骨成骨能力强,能有效促进骨愈合,是修复大段骨缺损的有效方法.
목적 평개혈관내피조세포(endothelial progenitor cells,EPCs)촉혈관화조직공정골수복토뇨골대단골결손적효과.방법 선용68지신서란대이백토,이수자수궤법분위3조.실험조:EPCs+경성골유도적골수간충질간세포(bone marrow mesenchymal stem cells,BMSCs)+탈개골기질(decalcified bone matrix,DBM);자신대조조:BMSCs+DBM;음성대조조:단순DBM.장상술재료치입뇨골중단15 mm골결손구,술후12,16주섭X선편행골밀도、조직학광경、골개소면역조화염색급생물역학측시.결과 실험조적골가생장、소형、수강재통、골유합속도급역학강도등방면균명현우우대조조.결론 EPCs촉혈관화조직공정골성골능력강,능유효촉진골유합,시수복대단골결손적유효방법.
Objective To evaluate the bone healing effect of vascularized tissue-engineered bone induced by endothelial progenitor cells(EPCs)in repair of large segmental radius defects in rabbits.Methods A total of 68 healthy New Zealand white rabbits were enrolled in the study and randomized into three groups,ie,experimental group(EPCs group):EPCs plus bone marrow mesenchymal stem cells (BMSCs)plus decalcified bone matrix(DBM);control group:BMSCs plus DBM;sham control group:pure DBM.Materials mentioned above were implanted into middle radius defects for 15 mm.At 12 and 16 weeks post-operatively,X-ray test,bone mineral density test,histological light microscopic test,osteocalcin immunohistochemical staining test and biomechanical test were carried out.Results Growth and plasticity of callus,speed of medullary cavity recanalization,bone healing speed and biomechanical intensity in the experimental group were all significantly better than those of control group.Conclusions Vascularized tissue-engineered bone induced by EPCs has strong osteegenic ability,can accelerate bone healing and hence is an effective method for repair of large segmental bone defects.