中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2010年
9期
764-769
,共6页
朱鹏立%贾德安%沈阳辉%阮景明%余惠珍%陈慧
硃鵬立%賈德安%瀋暘輝%阮景明%餘惠珍%陳慧
주붕립%가덕안%침양휘%원경명%여혜진%진혜
动脉粥样硬化%巨噬细胞%基质金属蛋白酶类
動脈粥樣硬化%巨噬細胞%基質金屬蛋白酶類
동맥죽양경화%거서세포%기질금속단백매류
Atherosclerosis%Macrophages%Matrix metalloproteinases
目的 探讨白藜芦醇抑制可溶性CD40配体(sCD40L)作用下对巨噬细胞基质金属蛋白酶-9(MMP-9)表达的影响.方法 佛波酯诱导人单核细胞株(THP-1)细胞分化为巨噬细胞.依次给予白藜芦醇和可溶性sCD40L孵育细胞,利用半定量反转录-聚合酶链反应(RT-PCR)、蛋白免疫印迹法、明胶酶谱法检测巨噬细胞内MMP-9和组织基质金属蛋白酶抑制因子-1(TIMP-1)基因的转录、蛋白表达和酶活性.结果 给予sCD40L刺激后巨噬细胞内MMP-9基因转录增多(1.53±0.04与0.75±0.01,P<0.05),蛋白分泌明显增加(244 930.8±31 268.6与192 976.8±20 223.1,P<0.05);白藜芦醇可抑制巨噬细胞MMP-9基因转录及蛋白分泌(P<0.01),降低MMP-9酶活性(P<0.05),升高巨噬细胞TIMP-1基因转录和蛋白分泌(P<0.05).结论 白藜芦醇可以抑制CD40途径活化的巨噬细胞内MMP-9的表达,调节MMP-9的活性,这可能是其抗动脉粥样硬化、稳定粥样斑块的作用机制之一.
目的 探討白藜蘆醇抑製可溶性CD40配體(sCD40L)作用下對巨噬細胞基質金屬蛋白酶-9(MMP-9)錶達的影響.方法 彿波酯誘導人單覈細胞株(THP-1)細胞分化為巨噬細胞.依次給予白藜蘆醇和可溶性sCD40L孵育細胞,利用半定量反轉錄-聚閤酶鏈反應(RT-PCR)、蛋白免疫印跡法、明膠酶譜法檢測巨噬細胞內MMP-9和組織基質金屬蛋白酶抑製因子-1(TIMP-1)基因的轉錄、蛋白錶達和酶活性.結果 給予sCD40L刺激後巨噬細胞內MMP-9基因轉錄增多(1.53±0.04與0.75±0.01,P<0.05),蛋白分泌明顯增加(244 930.8±31 268.6與192 976.8±20 223.1,P<0.05);白藜蘆醇可抑製巨噬細胞MMP-9基因轉錄及蛋白分泌(P<0.01),降低MMP-9酶活性(P<0.05),升高巨噬細胞TIMP-1基因轉錄和蛋白分泌(P<0.05).結論 白藜蘆醇可以抑製CD40途徑活化的巨噬細胞內MMP-9的錶達,調節MMP-9的活性,這可能是其抗動脈粥樣硬化、穩定粥樣斑塊的作用機製之一.
목적 탐토백려호순억제가용성CD40배체(sCD40L)작용하대거서세포기질금속단백매-9(MMP-9)표체적영향.방법 불파지유도인단핵세포주(THP-1)세포분화위거서세포.의차급여백려호순화가용성sCD40L부육세포,이용반정량반전록-취합매련반응(RT-PCR)、단백면역인적법、명효매보법검측거서세포내MMP-9화조직기질금속단백매억제인자-1(TIMP-1)기인적전록、단백표체화매활성.결과 급여sCD40L자격후거서세포내MMP-9기인전록증다(1.53±0.04여0.75±0.01,P<0.05),단백분비명현증가(244 930.8±31 268.6여192 976.8±20 223.1,P<0.05);백려호순가억제거서세포MMP-9기인전록급단백분비(P<0.01),강저MMP-9매활성(P<0.05),승고거서세포TIMP-1기인전록화단백분비(P<0.05).결론 백려호순가이억제CD40도경활화적거서세포내MMP-9적표체,조절MMP-9적활성,저가능시기항동맥죽양경화、은정죽양반괴적작용궤제지일.
Objective To explore the effect of resveratrol on the expression of matrix metalloproteinases-9 (MMP-9) in soluble CD40 ligand (sCD40L)-activated macrophages. Methods Human monocytic cell line THP-1 cells under an inducing of phorbol ester differentiated into macrophages. Then the macrophages were sitimulated by sCD40L independently and after a preincubation with resveratrol. The mRNA expression of MMP-9 and tissue-inhibitor of metalloproteinase-1 (TIMP-1) in macrophages were investigated by semiquantitative RT-PCR. The secretions of MMP-9 and TIMP-1 protein were measured by Western blot. The MMP-9 activity was analyzed by gelatin zymography technique. Results The expressions of MMP-9 gene(1.53±0.04 vs. 0.75±0.01,P＜0.05) and protein(244 930.8±31 268.6 vs. 192 976.8±20 223.1,P＜0.05)were higher in macrophages when stimulated by sCD40L. Resveratrol (10 μmol/L and 50 μmol/L)can inhibit the CD40L-induced gene expression and the protein secretion of MMP-9 (P＜0.01). The activity of MMP-9 was degraded by resveratrol (P＜0.05). Meanwhile resveratrol could increase the gene expression and protein secretion of TIMP-1 (P＜0.05). Conclusions Resveratrol can inhibit the CD40L-activated macrophage expression of MMP-9. It may be one of its mechanisms on antiatherosclerosis and stabilization of atheromatous plaques.
