中华精神科杂志
中華精神科雜誌
중화정신과잡지
CHINESE JOURNA OF PSYCHIATRY
2011年
2期
75-78
,共4页
李彩霞%王强%邓伟%马小红%王英成%刘协和%李涛
李綵霞%王彊%鄧偉%馬小紅%王英成%劉協和%李濤
리채하%왕강%산위%마소홍%왕영성%류협화%리도
精神分裂症%吸烟%基因%受体,烟碱
精神分裂癥%吸煙%基因%受體,煙堿
정신분렬증%흡연%기인%수체,연감
Schizophrenia%Smoking%Genes%Receptors,nicotinic
目的 探讨α7烟碱样乙酰胆碱受体(nAchR)基因多态性与精神分裂症及吸烟行为的关系.方法 连续收集符合美国精神障碍诊断与统计手册第4版(DSM-IV)诊断标准的精神分裂症患者221例(患者组)和患者亲属432名(亲属组),评定患者吸烟情况;采用聚合酶链反应和限制性酶切技术,检测α7nAchR基因的rs1355920单核苷酸多态性(SNP)和D15S1360微卫星多态性2个位点,对其基因型和等位基因与精神分裂症发病以及吸烟行为的关系进行传递不平衡检验和单倍型分析.结果 患者组从亲代接受到SNP rs1355920位点等位基因A的几率增加(Z=2.56,P=0.010)、接受到等位基因G的几率减少(Z=-2.70,P=0.007),SNP rs1355920与精神分裂症的发病有关(总体x2值=6.910,总体P值=0.008),微卫星多态性位点D15S1360与精神分裂症发病无关(总体x2值=1.331,总体P值=0.514),2个位点单倍型与精神分裂症发病有关(总体x2值=6.991,总体P值=0.030);吸烟组与不吸烟组在rs1355920和D15S1360多态性位点的基因型和等位基因的差异均无统计学意义(P>0.05).结论 α7nAchR基因可能与精神分裂症的发病存在关联,可能与吸烟行为不存在关联.
目的 探討α7煙堿樣乙酰膽堿受體(nAchR)基因多態性與精神分裂癥及吸煙行為的關繫.方法 連續收集符閤美國精神障礙診斷與統計手冊第4版(DSM-IV)診斷標準的精神分裂癥患者221例(患者組)和患者親屬432名(親屬組),評定患者吸煙情況;採用聚閤酶鏈反應和限製性酶切技術,檢測α7nAchR基因的rs1355920單覈苷痠多態性(SNP)和D15S1360微衛星多態性2箇位點,對其基因型和等位基因與精神分裂癥髮病以及吸煙行為的關繫進行傳遞不平衡檢驗和單倍型分析.結果 患者組從親代接受到SNP rs1355920位點等位基因A的幾率增加(Z=2.56,P=0.010)、接受到等位基因G的幾率減少(Z=-2.70,P=0.007),SNP rs1355920與精神分裂癥的髮病有關(總體x2值=6.910,總體P值=0.008),微衛星多態性位點D15S1360與精神分裂癥髮病無關(總體x2值=1.331,總體P值=0.514),2箇位點單倍型與精神分裂癥髮病有關(總體x2值=6.991,總體P值=0.030);吸煙組與不吸煙組在rs1355920和D15S1360多態性位點的基因型和等位基因的差異均無統計學意義(P>0.05).結論 α7nAchR基因可能與精神分裂癥的髮病存在關聯,可能與吸煙行為不存在關聯.
목적 탐토α7연감양을선담감수체(nAchR)기인다태성여정신분렬증급흡연행위적관계.방법 련속수집부합미국정신장애진단여통계수책제4판(DSM-IV)진단표준적정신분렬증환자221례(환자조)화환자친속432명(친속조),평정환자흡연정황;채용취합매련반응화한제성매절기술,검측α7nAchR기인적rs1355920단핵감산다태성(SNP)화D15S1360미위성다태성2개위점,대기기인형화등위기인여정신분렬증발병이급흡연행위적관계진행전체불평형검험화단배형분석.결과 환자조종친대접수도SNP rs1355920위점등위기인A적궤솔증가(Z=2.56,P=0.010)、접수도등위기인G적궤솔감소(Z=-2.70,P=0.007),SNP rs1355920여정신분렬증적발병유관(총체x2치=6.910,총체P치=0.008),미위성다태성위점D15S1360여정신분렬증발병무관(총체x2치=1.331,총체P치=0.514),2개위점단배형여정신분렬증발병유관(총체x2치=6.991,총체P치=0.030);흡연조여불흡연조재rs1355920화D15S1360다태성위점적기인형화등위기인적차이균무통계학의의(P>0.05).결론 α7nAchR기인가능여정신분렬증적발병존재관련,가능여흡연행위불존재관련.
Objective To investigate the associations of two polymorphisms in α7 nicotinic acetylcholine receptor subunit gene(α7 nAchR)with schizophrenia and smoking.Methods Eligible patients with schizophrenia(n=221)according to the Diagnostic and Statistical Manual of Mental Disorders Fouah Edition(DSM-IV)and their healthy relatives(n=432)were continuously recruited.Smoking data were collected in the patients.Two polymorphisms(rs1355920 and D15S1360)of α7 nAchR gene were genotyped using PCR-RFLP.Associations of the polymorphisms with schizophrenia and smoking were analyzed using transmission disequilibrium tests(TDT)and haplotype analysis.Results The odds of patient group received from parents allele A of SNP rs1355920 was increased(Z=2.56,P=0.010),and the odds of received allele G was decreased(Z=-2.70,P=0.007).The allele A of rs1355920 was more common in the patients than in the relatives(x2=6.910,P=0.008).The frequencies of D15S1360 were not significantly different between patients and relatives(x2=1.33 1,P=0.5 14).The haplotypes of the two locus were related to schizophrenia(x2=6.991,P=0.030).In addition,all these alleles or genotype frequencies of rs1355920 and D15S1360 did not differ significantly between smokers and non-smokers(P>0.05).Conclusion The α7nAchR gene may be associated with schizophrenia.but not with smoking.
