CAJ | 학술논문

目的探讨外周血Vγ2Vδ2+T淋巴细胞数与功能变化对HIV合并结核感染状态的影响.方法将76例HIV/AIDS合并结核感染患者分为活动性结核感染组(HIV+TB组)和潜伏结核感染组(HIV+LTB组).流式细胞仪测定外周血淋巴细胞分类情况.酶联斑点免疫法(ELISPOT)和胞内细胞因子染色(ICS)方法检测在PPD和磷酸化抗原(HMBPP)的刺激下T淋巴细胞亚群分泌IFN-γ功能的情况.统计学处理采用t检验.结果 HIV+TB组CD3+T淋巴细胞绝对计数(t=-3.67,P<0.01)和Vγ2Vδ2+T淋巴细胞所占CD3+T淋巴细胞比例(t=-2.06,P<0.05)均显著低于HIV+LTB组.PPD刺激时,HIV+LTB组分泌特异性IFN-γ的T淋巴细胞和参与分泌IFN-γ的CD4+T淋巴细胞所占CD3+T淋巴细胞比例,与HIV+TB组比较差异均无统计学意义.HMBPP刺激时,HIV+LTB组与HIV+TB组比较,分泌IFN-γ的特异性T淋巴细胞计数(t=2.71,P<0.01)和产生IFN-7的特异性Vy2'T淋巴细胞比例(t=3.003,P<0.01)均显著增加.结论 Vγ2Vδ2+T淋巴细胞在HIV/AIDS合并活动性结核患者中的数量和功能都有受损,提示该类细胞在CD4+T淋巴细胞受到抑制的情况下可能是人体内抵御结核感染的重要免疫细胞.
목적 탐토외주혈Vγ2Vδ2+T림파세포수여공능변화대HIV합병결핵감염상태적영향.방법 장76례HIV/AIDS합병결핵감염환자분위활동성결핵감염조(HIV+TB조)화잠복결핵감염조(HIV+LTB조).류식세포의측정외주혈림파세포분류정황.매련반점면역법(ELISPOT)화포내세포인자염색(ICS)방법 검측재PPD화린산화항원(HMBPP)적자격하T림파세포아군분비IFN-γ공능적정황.통계학처리채용t검험.결과 HIV+TB조CD3+T림파세포절대계수(t=-3.67,P<0.01)화Vγ2Vδ2+T림파세포소점CD3+T림파세포비례(t=-2.06,P<0.05)균현저저우HIV+LTB조.PPD자격시,HIV+LTB조분비특이성IFN-γ적T림파세포화삼여분비IFN-γ적CD4+T림파세포소점CD3+T림파세포비례,여HIV+TB조비교차이균무통계학의의.HMBPP자격시,HIV+LTB조여HIV+TB조비교,분비IFN-γ적특이성T림파세포계수(t=2.71,P<0.01)화산생IFN-7적특이성Vy2'T림파세포비례(t=3.003,P<0.01)균현저증가.결론 Vγ2Vδ2+T림파세포재HIV/AIDS합병활동성결핵환자중적수량화공능도유수손,제시해류세포재CD4+T림파세포수도억제적정황하가능시인체내저어결핵감염적중요면역세포.
Objective To evaluate the number and function of peripheral Vγ2Vδ2+T lymphocytes during Mycobacterium tuberculosis(MTB)infection in human immunodeficiency virus (HIV)infected individuals.Methods Seventy-six HIV/acquired immune deficiency syndrome(AIDS) patients co-infected with MTB were divided into active tuberculosis(TB)group(HIV+TB)and latent TB group(HIV+LTB).T cell subsets of peripheral blood lymphocytes were analysed by flow cytometry.Stimulated by protein purified derivative(PPD)and hydroxymethylbutenyl diphosphate (HMBPP),specific interferon(IFN)-γ producing T cells were detected using enzyme-linked immunospot(ELISPOT)and intracellular cytokine staining(ICS).Data were analyzed by t test.Results The absolute number of CD3'T cells and DroDortion of V72VB2'T cells in CD3+T cells in HIV+TB group were both significantly lower than those in HIV+LTB group(t=-3.67,P<0.01;t=-2.06,P<0.05).PPD-specific IFN-y-producing T cells and percentage of PPD-specific CD4+Tcells in CD3+T cells in HIV+LTB group were both similar with those in HIV+TB group.While HMBPP-specific IFN-γ-producing T cells and percentage of HMBPP-specific Vγ2Vδ2+T cells in CD3+Tcells in HIV+LTB group were both higher than those in HIV+TB group(t=2.71 and t=3.003,respectively;both P<0.0 1).Conclusion The number and function of Vγ2Vδ2+T cells were impaired in HIV/AIDS patients coinfected with active MTB infection,which indicates that Vγ2Vδ2+T cells may be the key immune cells against MTB in individuals with impaired CD4+T cells.