中华临床营养杂志
中華臨床營養雜誌
중화림상영양잡지
CHINESE JOURNAL OF CLINICAL NUTRITION
2010年
4期
230-234,后插4
,共6页
向军英%欧阳钦%胡仁伟%甘华田
嚮軍英%歐暘欽%鬍仁偉%甘華田
향군영%구양흠%호인위%감화전
溃疡性结肠炎%白芍总苷%核因子-κB%肿瘤坏死因子-α
潰瘍性結腸炎%白芍總苷%覈因子-κB%腫瘤壞死因子-α
궤양성결장염%백작총감%핵인자-κB%종류배사인자-α
Ulcerative colitis%Total glucosides of paeony%Nuclear factor-κB%Tumor necrosis factor-α
目的 探讨白芍总苷(TGP)对小鼠溃疡性结肠炎(UC)的作用及机制.方法 将48只雄性BALB/c小鼠随机分为6组(n=8):正常对照组、模型组、柳氮磺胺吡啶(SASP)组(100mg·kg-1·d-1)以及低、中、高剂量TGP(60、120、240 mg·kg-1·d-1)组.除正常对照组外,其他组小鼠均以恶唑酮灌肠造模.灌肠1 d后,SASP组和TGP组开始给予相应的药物灌胃治疗3 d,同时进行疾病活动指数(DAI)评分.处死小鼠后,行结肠大体损伤和组织学损伤评分,以ELISA方法检测结肠黏膜中肿瘤坏死因子-α(TNF-α)水平,以免疫组织化学方法检测结肠黏膜中核因子-κB(NF-κB)p65的表达.结果 模型组小鼠DAI评分明显高于正常对照组(P＜0.01),与低剂量TGP治疗组相似(P＞0.05),SASP组、中剂量和高剂量TGP治疗组小鼠DAI评分明显低于模型组(P均＜0.05);模型组结肠大体评分、组织学评分、TNF-α水平和NF-κB p65的表达均显著高于正常对照组、SASP组、中剂量和高剂量TGP治疗组(P均＜0.01),与低剂量TGP治疗组相似(P＞0.05);高剂量TGP治疗组疗效优于SASP组.结论 TGP对实验性UC有一定的治疗作用,可能与其抑制NF-κB的活化,减少TNF-α的产生有关.
目的 探討白芍總苷(TGP)對小鼠潰瘍性結腸炎(UC)的作用及機製.方法 將48隻雄性BALB/c小鼠隨機分為6組(n=8):正常對照組、模型組、柳氮磺胺吡啶(SASP)組(100mg·kg-1·d-1)以及低、中、高劑量TGP(60、120、240 mg·kg-1·d-1)組.除正常對照組外,其他組小鼠均以噁唑酮灌腸造模.灌腸1 d後,SASP組和TGP組開始給予相應的藥物灌胃治療3 d,同時進行疾病活動指數(DAI)評分.處死小鼠後,行結腸大體損傷和組織學損傷評分,以ELISA方法檢測結腸黏膜中腫瘤壞死因子-α(TNF-α)水平,以免疫組織化學方法檢測結腸黏膜中覈因子-κB(NF-κB)p65的錶達.結果 模型組小鼠DAI評分明顯高于正常對照組(P＜0.01),與低劑量TGP治療組相似(P＞0.05),SASP組、中劑量和高劑量TGP治療組小鼠DAI評分明顯低于模型組(P均＜0.05);模型組結腸大體評分、組織學評分、TNF-α水平和NF-κB p65的錶達均顯著高于正常對照組、SASP組、中劑量和高劑量TGP治療組(P均＜0.01),與低劑量TGP治療組相似(P＞0.05);高劑量TGP治療組療效優于SASP組.結論 TGP對實驗性UC有一定的治療作用,可能與其抑製NF-κB的活化,減少TNF-α的產生有關.
목적 탐토백작총감(TGP)대소서궤양성결장염(UC)적작용급궤제.방법 장48지웅성BALB/c소서수궤분위6조(n=8):정상대조조、모형조、류담광알필정(SASP)조(100mg·kg-1·d-1)이급저、중、고제량TGP(60、120、240 mg·kg-1·d-1)조.제정상대조조외,기타조소서균이악서동관장조모.관장1 d후,SASP조화TGP조개시급여상응적약물관위치료3 d,동시진행질병활동지수(DAI)평분.처사소서후,행결장대체손상화조직학손상평분,이ELISA방법검측결장점막중종류배사인자-α(TNF-α)수평,이면역조직화학방법검측결장점막중핵인자-κB(NF-κB)p65적표체.결과 모형조소서DAI평분명현고우정상대조조(P＜0.01),여저제량TGP치료조상사(P＞0.05),SASP조、중제량화고제량TGP치료조소서DAI평분명현저우모형조(P균＜0.05);모형조결장대체평분、조직학평분、TNF-α수평화NF-κB p65적표체균현저고우정상대조조、SASP조、중제량화고제량TGP치료조(P균＜0.01),여저제량TGP치료조상사(P＞0.05);고제량TGP치료조료효우우SASP조.결론 TGP대실험성UC유일정적치료작용,가능여기억제NF-κB적활화,감소TNF-α적산생유관.
Objective To investigate the effects of total glucosides of paeony (TGP) on mice with experimental ulcerative colitis and the underlying mechanisms. Methods Forty-eight mice were equally randomized into 6 groups ( n = 8): normal control group, model group, salicylazosulfapyridine (SASP) group, low-dose TGP group one day after enama, the mice were treated with corresponding agent by oral gavage for3 days. The disease activity index (DAI) score was evaluated every day. After all the mice were scarified, the macroscopic and histological scores were evaluated. The tumor necrosis factor-α (TNF-α) level of the colon mucosa was measured by ELISA and the colonic expression of nuclear factor-κB (NF-κB) p65 was detected by immunohistochemistry. Results Compared with the normal control group, the DAI score was significantly higher in the model group (P ＜0. 01 ),but was similar to that in low-dose TGP group ( P ＞ 0.05 ). Compared with the model group, the DAI score was significantly decreased in SASP group, medium-dose TGP group, and high-dose TGP group (all P ＜ 0. 05). The macroscopic score, histological score, TNF-α level, and expression of NF-κB p65 were significantly higher in model group than in normal control group, SASP group, medium-dose TGP group, and high-dose TGP group ( all P ＜0. 01 ), but was similar to that in low-dose TGP group (P ＞ 0. 05). The efficacy of TGP was higher in high-dose TGP group than in SASP group. Conclusions TGP has certain therapeutic effects on experimental ulcerative colitis,which may be achieved by its inhibitory effect on the activation of NF-κB and the production of TNF-α.
