CAJ | 학술논문

目的采用不同相对分子质量枝化状聚乙二醇(PEG)交联巯基化的猪去细胞瓣,观察其对去细胞瓣膜力学性能的影响.方法将25例猪去细胞主动脉瓣随机分为5组:PEG3400组、PEG8000组、PEG12000组、PEG20000组和对照组,常温反应时间4 h.测定残余巯基计算交联效率;静态拉伸实验测定力学性能.结果 4 h交联率依次为92.40%、89.88%、87.87%和87.46%,相对分子质量越小,交联效率越高,PEG3400组与其他各组比较,差异有统计学意义(P＜0.05);力学测试显示,相对分子质量越大,力学强度越高,呈一定的正相关,PEG12000和PEG20000组抗拉强度分别为(3.22±0.41)MPa和(3.19±0.15)MPa,与对照组比较,差异有统计学意义(P＜0.05).结论相对分子质量12000 Da和20000 Da枝化状聚乙二醇交联可有效改善猪去细胞瓣的力学性能,有望用于构建新型复合的组织工程瓣膜支架材料的研究应用.
목적 채용불동상대분자질량지화상취을이순(PEG)교련구기화적저거세포판,관찰기대거세포판막역학성능적영향.방법 장25례저거세포주동맥판수궤분위5조:PEG3400조、PEG8000조、PEG12000조、PEG20000조화대조조,상온반응시간4 h.측정잔여구기계산교련효솔;정태랍신실험측정역학성능.결과 4 h교련솔의차위92.40%、89.88%、87.87%화87.46%,상대분자질량월소,교련효솔월고,PEG3400조여기타각조비교,차이유통계학의의(P＜0.05);역학측시현시,상대분자질량월대,역학강도월고,정일정적정상관,PEG12000화PEG20000조항랍강도분별위(3.22±0.41)MPa화(3.19±0.15)MPa,여대조조비교,차이유통계학의의(P＜0.05).결론 상대분자질량12000 Da화20000 Da지화상취을이순교련가유효개선저거세포판적역학성능,유망용우구건신형복합적조직공정판막지가재료적연구응용.
Objective Branched polyethylene glycols (PEG) with different molecular weight were cross-linked to the porcine decellularized aortic valve (DAV) and the effects of PEG cross-linking on the mechanical properties were investigated. Methods A total of 25 porcine DAVs were randomly assigned into 5 groups: PEG3400, PEG8000, PEG12000, PEG20000 and control. The reactive time was 4 h at room temperature. The efficiency of crosslinking was calculated by measuring the residual thiol group. The mechanical properties were obtained by static tensile test. Results The efficiency of crosslinking was 92. 40% , 89. 88% , 87. 87% and 87. 46% in PEG3400, PEG8000, PEG12000, PEG20000 groups, respectively. As compared with other groups, the PEG3400 group had significantly greater increase in the crosslinking degree (P ＜ 0. 05). Tensile test showed the tensile strength of PEG12000 and PEG20000 groups was (3. 22 ±0.41) and (3. 19 ±0. 15) MPa, respectively, and significantly different from that in control group (P ＜ 0. 05). The tensile strength and young' s modulus had a positive correlation with the molecular weight of PEG. Conclusion Branched PEG with the molecular weight of 12 000 Da and 20 000 Dacan effectively crosslink to the porcine decellularized aortic valves and improve their mechanical behaviors, and has the potential to be used as the material of fabricating new hybrid scaffold of tissue engineering heart valves.