CAJ | 학술논문

目的观察白蛋白对肾小管上皮细胞血管紧张素转换酶(ACE)mRNA和蛋白水平表达的影响,并探讨尿蛋白激活肾脏局部.肾素.血管紧张索系统(RAS)的机制.方法分别采用2.5、5、10 g/L的牛血清白蛋白(BSA)刺激人近端肾小管上皮细胞株(HK-2)6 h和12 h.并分别采用实时定量PCR和Western印迹检测ACE mRNA和蛋白水平的表达.结果与对照组相比,随着BSA刺激浓度的增加,HK-2细胞ACE mRNA表达均显著增加(均P<0.05).同时,Western印迹显示ACE蛋白表达也均显著增加(均P<0.05).另外,BSA 10 g/L作用于HK-2细胞6 h和12 h后,ACE mRNA表达显著增加(均P<0.05);Western印迹显示ACE蛋白也显著增加(均P<0.05).结论 BSA可显著增加HK-2细胞ACE表达,此作用可能是导致肾间质局部Ang Ⅱ蓄积从而启动肾小管间质纤维化的重要机制之一.
목적 관찰백단백대신소관상피세포혈관긴장소전환매(ACE)mRNA화단백수평표체적영향,병탐토뇨단백격활신장국부.신소.혈관긴장색계통(RAS)적궤제.방법 분별채용2.5、5、10 g/L적우혈청백단백(BSA)자격인근단신소관상피세포주(HK-2)6 h화12 h.병분별채용실시정량PCR화Western인적검측ACE mRNA화단백수평적표체.결과 여대조조상비,수착BSA자격농도적증가,HK-2세포ACE mRNA표체균현저증가(균P<0.05).동시,Western인적현시ACE단백표체야균현저증가(균P<0.05).령외,BSA 10 g/L작용우HK-2세포6 h화12 h후,ACE mRNA표체현저증가(균P<0.05);Western인적현시ACE단백야현저증가(균P<0.05).결론 BSA가현저증가HK-2세포ACE표체,차작용가능시도치신간질국부Ang Ⅱ축적종이계동신소관간질섬유화적중요궤제지일.
Objective To investigate the influence of albumin on the expression of angiotensin-eonverting enzyme (ACE) in cultured human proximal tubular ceils (HK-2). Methods HK-2 cells were exposed to 2.5, 5, 10 g/L bovine serum albumin (BSA) for 6 h and 12 h respectively. The expression of ACE in HK-2 cells was detected by real-time RT-PCR and Western blot. Results Compared to the control group, the expression of HK-2 cells ACE mRNA treated for 12 h with different concentrations of BSA (2.5, 5, 10 g/L) significantly increased (P<O.05). Furthermore, Western blot analysis showed that the expression of ACE protein induced by BSA significantly increased (P<0.05). Treated with BSA (10 g/L) for 6 h and 12 h, the expression of ACE mRNA significantly increased in a time-dependent manner (P <0.05, respectively), and the ACE protein expression significantly increased (P<0.05, respectively). Conclusion BSA can induce ACE up-regulation in proximal tubular cells, which may lead to the increased production of local Ang Ⅱ and finally contributes to the intrarenal activation of renin angiotensin system (RAS).