上海交通大学学报（医学版）
上海交通大學學報（醫學版）
상해교통대학학보（의학판）
JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY(MEDICAL SCIENCE)
2009年
10期
1200-1204
,共5页
墨旱莲%环磷酰胺%CCl_4%扶正固本%保肝
墨旱蓮%環燐酰胺%CCl_4%扶正固本%保肝
묵한련%배린선알%CCl_4%부정고본%보간
Eclipta L.%cyclophosphamide%CCl_4%strengthening and restoring effects%hepatoprotective effect
目的 评价中药墨旱莲的扶正固本和保肝作用.方法 60只昆明小鼠随机分为对照组,墨旱莲高、低剂量组,环磷酰胺模型组,环磷酰胺(CY)模型的墨旱莲高、低剂量组,每组10只.以8 g·kg~(-1)·d~(-1)和4 g·kg~(-1)·d~(-1)剂量的墨旱莲灌胃给药7 d,给药第4天腹腔注射0.05 g/kg剂量的CY建立免疫低下模型,观察墨旱莲提取物对正常小鼠和CY诱导的免疫低下小鼠的胸腺指数、脾脏指数、碳粒廓清指数的影响;取BALB/c小鼠2只观察墨旱莲提取物对淋巴细胞增殖作用和ConA诱导小鼠淋巴细胞转化增殖作用的影响,评价墨旱莲扶正固本作用.70只昆明小鼠随机分为对照组,墨旱莲高、低剂量组,CCl_4模型组,联苯双酯(DDB)阳性对照组,CCl_4模型的墨旱莲高、低剂量组,每组10只小鼠.以20 g·kg~(-1)·d~(-1)和10 g·kg~(-1)·d~(-1)剂量的墨旱莲灌胃给药7 d,末次给药后2 h腹腔注射10 mL/kg剂量的CCl_4建立肝损伤模型,观察墨旱莲提取物对正常小鼠和CCl_4诱导的肝损伤小鼠血清ALT、MDA、TP、T-Bil值的影响;取SD大鼠1只,观察墨旱莲提取物对大鼠原代肝细胞活力的影响,评价墨旱莲的保肝作用.结果 墨旱莲提取物能上调正常小鼠和CY诱导的免疫低下小鼠的胸腺指数和碳粒廓清指数;能促进小鼠淋巴细胞增殖以及ConA诱导的T淋巴细胞转化增殖;能降低肝损伤小鼠的血清ALT、MDA值;能增强原代培养肝细胞的活力.结论 中药墨旱莲具有扶正固本作用和保肝作用.
目的 評價中藥墨旱蓮的扶正固本和保肝作用.方法 60隻昆明小鼠隨機分為對照組,墨旱蓮高、低劑量組,環燐酰胺模型組,環燐酰胺(CY)模型的墨旱蓮高、低劑量組,每組10隻.以8 g·kg~(-1)·d~(-1)和4 g·kg~(-1)·d~(-1)劑量的墨旱蓮灌胃給藥7 d,給藥第4天腹腔註射0.05 g/kg劑量的CY建立免疫低下模型,觀察墨旱蓮提取物對正常小鼠和CY誘導的免疫低下小鼠的胸腺指數、脾髒指數、碳粒廓清指數的影響;取BALB/c小鼠2隻觀察墨旱蓮提取物對淋巴細胞增殖作用和ConA誘導小鼠淋巴細胞轉化增殖作用的影響,評價墨旱蓮扶正固本作用.70隻昆明小鼠隨機分為對照組,墨旱蓮高、低劑量組,CCl_4模型組,聯苯雙酯(DDB)暘性對照組,CCl_4模型的墨旱蓮高、低劑量組,每組10隻小鼠.以20 g·kg~(-1)·d~(-1)和10 g·kg~(-1)·d~(-1)劑量的墨旱蓮灌胃給藥7 d,末次給藥後2 h腹腔註射10 mL/kg劑量的CCl_4建立肝損傷模型,觀察墨旱蓮提取物對正常小鼠和CCl_4誘導的肝損傷小鼠血清ALT、MDA、TP、T-Bil值的影響;取SD大鼠1隻,觀察墨旱蓮提取物對大鼠原代肝細胞活力的影響,評價墨旱蓮的保肝作用.結果 墨旱蓮提取物能上調正常小鼠和CY誘導的免疫低下小鼠的胸腺指數和碳粒廓清指數;能促進小鼠淋巴細胞增殖以及ConA誘導的T淋巴細胞轉化增殖;能降低肝損傷小鼠的血清ALT、MDA值;能增彊原代培養肝細胞的活力.結論 中藥墨旱蓮具有扶正固本作用和保肝作用.
목적 평개중약묵한련적부정고본화보간작용.방법 60지곤명소서수궤분위대조조,묵한련고、저제량조,배린선알모형조,배린선알(CY)모형적묵한련고、저제량조,매조10지.이8 g·kg~(-1)·d~(-1)화4 g·kg~(-1)·d~(-1)제량적묵한련관위급약7 d,급약제4천복강주사0.05 g/kg제량적CY건립면역저하모형,관찰묵한련제취물대정상소서화CY유도적면역저하소서적흉선지수、비장지수、탄립곽청지수적영향;취BALB/c소서2지관찰묵한련제취물대림파세포증식작용화ConA유도소서림파세포전화증식작용적영향,평개묵한련부정고본작용.70지곤명소서수궤분위대조조,묵한련고、저제량조,CCl_4모형조,련분쌍지(DDB)양성대조조,CCl_4모형적묵한련고、저제량조,매조10지소서.이20 g·kg~(-1)·d~(-1)화10 g·kg~(-1)·d~(-1)제량적묵한련관위급약7 d,말차급약후2 h복강주사10 mL/kg제량적CCl_4건립간손상모형,관찰묵한련제취물대정상소서화CCl_4유도적간손상소서혈청ALT、MDA、TP、T-Bil치적영향;취SD대서1지,관찰묵한련제취물대대서원대간세포활력적영향,평개묵한련적보간작용.결과 묵한련제취물능상조정상소서화CY유도적면역저하소서적흉선지수화탄립곽청지수;능촉진소서림파세포증식이급ConA유도적T림파세포전화증식;능강저간손상소서적혈청ALT、MDA치;능증강원대배양간세포적활력.결론 중약묵한련구유부정고본작용화보간작용.
Objective To evaluate the strengthening and restoring effects and hepatoprotective effect of Eclipta extracts. Methods Sixty Kunming mice were randomly divided into control group, low-dose Eclipta group, high-dose Eclipta group, cyclopho9phamide(CY) group, low-dose Eclipta CY group and high-dose Eclipta CY group(n = 10). Eclipta 8 g·kg~(-1)d~(-1) or 4 g·kg~(-1) · d~(-1) were administered by oral gavage for 7 d. Immunosuppression mouse models were established by intraperitoneal injection of 0. 05 g/kg CY on the fourth day of administration. The effects of Eclipta extracts on thymus index, spleen index and carbon clearance index in both normal mice and immunosuppression mice induced by CY were tested, and those on proliferation of lymphocyte and T cells induced by ConA were examined. Based on these data, strengthening and restoring effects of Eclipta were observed. Besides, 70 Kunming mice were randomly divided into control group, low-dose Eclipta group, high-dose Eclipta group, CCl_4 group, DDB group, low-dose Eclipta CCl_4 group and high-dose Eclipta CCl_4 group. 20 g·kg~(-1)·d~(-1) and 10 g·kg~(-1) ·d~(-1) Eclipta were administered by oral gavage for 7 d. Hepatic injury mouse models were established by intraperitoneal injection of 10 mL/kg CCl_4 2 h after the last administration. The effects of Eclipta extracts on serum ALT, MDA, TP and T-Bil levels in both normal mice and hepatic injury mice induced by CCl_4 was tested, and those on the viability of the primary culture hepatocytes were determined. Based on these data, hepatoprotective effect of Eclipta was observed. Results Eclipta extracts increased thymus index and carbon clearance index in both normal mice and immunosuppression mice induced by CY, promoted proliferation of lymphocyte and proliferation of T cells induced by ConA, reduced serum ALT and MDA levels in hepatic injury mice, and improved the viability of the primary culture hepatocytes. Conclusion Eclipta herb has strengthening and restoring effects and hepatoprotective effect.
