CAJ | 학술논문

目的探讨三氧化二砷(As2O3)和曲古抑菌素A(TSA)在体外诱导急性早幼粒白血病细胞HL-60凋亡过程中的协同作用.方法采用MTT法检测As2O3和(或)TSA对HL-60细胞增殖的影响,流式细胞术检测细胞周期和细胞凋亡,逆转录-聚合酶链反应(RT-PCR)检测凋亡相关基因Bax和Bcl-2的表达.结果 As2O3和TSA联用较单独用药能明显抑制细胞,引起细胞周期阻滞和凋亡,Bax基因表达升高,Bcl-2基因表达降低,Bax/Bcl-2比值升高.结论 As2O3和TSA均能够引起HL-60凋亡,二者具有协同效应,其机制是引起细胞周期阻滞,且上调Bax与Bcl-2比值.
목적 탐토삼양화이신(As2O3)화곡고억균소A(TSA)재체외유도급성조유립백혈병세포HL-60조망과정중적협동작용.방법 채용MTT법검측As2O3화(혹)TSA대HL-60세포증식적영향,류식세포술검측세포주기화세포조망,역전록-취합매련반응(RT-PCR)검측조망상관기인Bax화Bcl-2적표체.결과 As2O3화TSA련용교단독용약능명현억제세포,인기세포주기조체화조망,Bax기인표체승고,Bcl-2기인표체강저,Bax/Bcl-2비치승고.결론 As2O3화TSA균능구인기HL-60조망,이자구유협동효응,기궤제시인기세포주기조체,차상조Bax여Bcl-2비치.
Objective To detect the synergetic effect and mechanism of arsenic trioxide(As2O3)and Trichostatin A(TSA)during inducing apoptosis of HL-60 cells.Methods MTT method was used to test the proliferation of HL-60 cells.Cell cycle and apoptosis were detected by FCM.Semi-quantitative RT-PCR was used to detect the mRNA expression of Bax and Bcl-2 in the cells treated by As2O3 and(or)TSA.Results As2O3 combined with TSA could inhibit proliferation and induce cell cycle arrest at G0 and G1.The percent of apoptosis induced by combination of As2O3 and TSA was obviously higher than that of either As2O3 or TSA.Bax gene expression was increased,while Bcl-2 gene expression was decreased,Bax/Bel-2 ratio was up-regulated.Conclusion Synergetic effect by As2O3 and TSA is remarkable in inducing apoptosis of HL-60 cells.Cell cycte arrest and Bax/Bcl-2 ratio play an important role in apoptosis of HL-60 cells induced by As2O3,TSA or their combination.