国际医学寄生虫病杂志
國際醫學寄生蟲病雜誌
국제의학기생충병잡지
INTERNATIONAL JOURNAL OF MEDICAL PARASITIC DISEASES
2010年
6期
323-326
,共4页
旋毛虫%成虫排泄分泌抗原%唾液%血清%ELISA
鏇毛蟲%成蟲排洩分泌抗原%唾液%血清%ELISA
선모충%성충배설분비항원%타액%혈청%ELISA
T. spiralis%Adult worm excretory-secretory antigen%Saliva%Serum%ELISA
目的 评价旋毛虫(Trichinella spiralis)成虫排泄分泌抗原(adult worm excretory-secretory antigen,AWESA)作为诊断抗原检测旋毛虫感染日本大耳兔唾液中抗旋毛虫IgG抗体的可行性. 方法 建立旋毛虫感染日本大耳兔和对照组兔动物模型,采集感染前和感染后1～6周兔唾液和血清以及对照组兔唾液和血清.制备AWESA,建立AWESA作为诊断抗原的间接酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA),以市售旋毛虫IgG抗体检测试剂盒作为对照,测定感染前和感染后1～6周兔唾液和血清以及对照组兔唾液和血清中抗旋毛虫IgG抗体.AWESA和试剂盒测得的唾液A值和血清A值进行线性相关分析,AWESA和试剂盒测得的唾液阳性率和血清阳性率分别进行x2检验.结果 AWESA检测唾液和血清特异性IgG抗体阳性率依次为0、5%、20%、40%、60%、85%、90%,0、30%、60%、85%、95%、100%、100%,除感染后0、1、2周外其余各周唾液A值与血清A值呈显著线性相关(P＞0.05、P＞0.05、P＞0.05、P＜0.05、P＜0.05、P＜0.05、P＜0.05).市售旋毛虫IgG抗体检测试剂盒检测旋毛虫感染前和感染后兔唾液和血清中特异性IgG抗体阳性率依次为0、15%、20%、40%、55%、75%、90%,0、35%、60%、95%、95%、100%、100%,除0、1、3周外其余各周唾液A值与血清A值呈线性相关(P＞0.05、P＞0.05、P＜0.05、P＞0.05、P＜0.05、P＜0.05、P＜0.05). 结论 AWESA与市售试剂盒检测唾液和血清中抗旋毛虫IgG抗体的阳性率具有一致性.
目的 評價鏇毛蟲(Trichinella spiralis)成蟲排洩分泌抗原(adult worm excretory-secretory antigen,AWESA)作為診斷抗原檢測鏇毛蟲感染日本大耳兔唾液中抗鏇毛蟲IgG抗體的可行性. 方法 建立鏇毛蟲感染日本大耳兔和對照組兔動物模型,採集感染前和感染後1～6週兔唾液和血清以及對照組兔唾液和血清.製備AWESA,建立AWESA作為診斷抗原的間接酶聯免疫吸附試驗(enzyme-linked immunosorbent assay,ELISA),以市售鏇毛蟲IgG抗體檢測試劑盒作為對照,測定感染前和感染後1～6週兔唾液和血清以及對照組兔唾液和血清中抗鏇毛蟲IgG抗體.AWESA和試劑盒測得的唾液A值和血清A值進行線性相關分析,AWESA和試劑盒測得的唾液暘性率和血清暘性率分彆進行x2檢驗.結果 AWESA檢測唾液和血清特異性IgG抗體暘性率依次為0、5%、20%、40%、60%、85%、90%,0、30%、60%、85%、95%、100%、100%,除感染後0、1、2週外其餘各週唾液A值與血清A值呈顯著線性相關(P＞0.05、P＞0.05、P＞0.05、P＜0.05、P＜0.05、P＜0.05、P＜0.05).市售鏇毛蟲IgG抗體檢測試劑盒檢測鏇毛蟲感染前和感染後兔唾液和血清中特異性IgG抗體暘性率依次為0、15%、20%、40%、55%、75%、90%,0、35%、60%、95%、95%、100%、100%,除0、1、3週外其餘各週唾液A值與血清A值呈線性相關(P＞0.05、P＞0.05、P＜0.05、P＞0.05、P＜0.05、P＜0.05、P＜0.05). 結論 AWESA與市售試劑盒檢測唾液和血清中抗鏇毛蟲IgG抗體的暘性率具有一緻性.
목적 평개선모충(Trichinella spiralis)성충배설분비항원(adult worm excretory-secretory antigen,AWESA)작위진단항원검측선모충감염일본대이토타액중항선모충IgG항체적가행성. 방법 건립선모충감염일본대이토화대조조토동물모형,채집감염전화감염후1～6주토타액화혈청이급대조조토타액화혈청.제비AWESA,건립AWESA작위진단항원적간접매련면역흡부시험(enzyme-linked immunosorbent assay,ELISA),이시수선모충IgG항체검측시제합작위대조,측정감염전화감염후1～6주토타액화혈청이급대조조토타액화혈청중항선모충IgG항체.AWESA화시제합측득적타액A치화혈청A치진행선성상관분석,AWESA화시제합측득적타액양성솔화혈청양성솔분별진행x2검험.결과 AWESA검측타액화혈청특이성IgG항체양성솔의차위0、5%、20%、40%、60%、85%、90%,0、30%、60%、85%、95%、100%、100%,제감염후0、1、2주외기여각주타액A치여혈청A치정현저선성상관(P＞0.05、P＞0.05、P＞0.05、P＜0.05、P＜0.05、P＜0.05、P＜0.05).시수선모충IgG항체검측시제합검측선모충감염전화감염후토타액화혈청중특이성IgG항체양성솔의차위0、15%、20%、40%、55%、75%、90%,0、35%、60%、95%、95%、100%、100%,제0、1、3주외기여각주타액A치여혈청A치정선성상관(P＞0.05、P＞0.05、P＜0.05、P＞0.05、P＜0.05、P＜0.05、P＜0.05). 결론 AWESA여시수시제합검측타액화혈청중항선모충IgG항체적양성솔구유일치성.
Objective To evaluate the feasibility of the detection of IgG antibodies in saliva with Trichinella spiralis adult worm excretory-secretory antigen (AWESA). Methods Animal model of Japanese big ears rabbits infected with T. spiralis was established, AWESA was prepared. Saliva and serum of the rabbits before and 1-6 weeks post infection and that of the control group were collected. Taking commercial kit detecting anti-T. spiralis IgG antibody as control, indirect enzyme-linked immunosorbent assay (ELISA) using AWESA was developed. Anti-T. spiralis IgG antibodies in saliva and serum of the rabbits before and 1-6 weeks post infection and that of the control group were detected with ELISA. A values of saliva and serum from the detection with AWESA and commercial kit were tested with linear regression analysis. The comparisons of positive rates from the detection with AWESA and commercial kit were determined by the chi-square test. Results The positive rates of saliva and serum from rabbits infected with T. spiralis before infection and 1-6 weeks postinfection obtained by AWESA were 0,5%, 20%, 40%, 60%, 85%, 90% and 0, 30%, 60%, 85%, 95%,100%, 100% ,respectively. There were significant linear correlations between A values of saliva and A values of serum every week, except for before and 1 week and 2 week post-infection ( P ＞ 0.05, P ＞ 0.05, P ＞ 0.05, P ＜0.05 ,P ＜0.05 ,P ＜0.05 ,P ＜0.05, respectively). The positive rates of saliva and serum from rabbits infected with T. spiralis before and 1-6 weeks post-infection obtained by commercial kit detecting anti-T. spiralis IgG antibody were 0, 15% ,20% ,40% ,55% ,75% ,90% and 0,35% ,60% ,95% ,95%, 100%, 100%, respectively.There were significant correlations between A values of saliva and A values of serum every week, except that of before infection, 1 week and 3 week post-infection ( P ＞ 0.05 、P ＞ 0.05 、P ＜ 0.05 、P ＞ 0.05 、P ＜ 0.05 、P ＜0.05 、P ＜ 0.05, respectively). Conclusion The positive rates of the detection of anti-T. spiralis IgG antibodies in saliva and serum by AWESA were of consistency with that by commercial kit.
