白血病·淋巴瘤
白血病·淋巴瘤
백혈병·림파류
JOURNAL OF LEUKEMIA & LYMPHOMA
2008年
2期
98-99,104
,共3页
程淑琴%陈成坚%谢伟成%谢必霞%黄朝辉%曹小龙
程淑琴%陳成堅%謝偉成%謝必霞%黃朝輝%曹小龍
정숙금%진성견%사위성%사필하%황조휘%조소룡
血液病%原位杂交,荧光%染色体%三体8
血液病%原位雜交,熒光%染色體%三體8
혈액병%원위잡교,형광%염색체%삼체8
Hematologic diseases%In situ hybridization,fluorescence%Chromosomes%Trisomy 8
目的 探讨间期荧光原位杂交(FISH)技术在检测血液病染色体三体8中的价值.方法 用荧光素直接标记的8号染色体着丝粒探针对77例血液病患者进行间期FISH检测,并与常规细胞遗传学方法(CC)进行比较分析.结果 FISH法三体8的检出率较传统吉姆萨显带高,或在吉姆萨显带无法分析时提供结果.结论 FISH检测三体8的敏感性高于常规核型分析,在小克隆检测方面有其优越性.
目的 探討間期熒光原位雜交(FISH)技術在檢測血液病染色體三體8中的價值.方法 用熒光素直接標記的8號染色體著絲粒探針對77例血液病患者進行間期FISH檢測,併與常規細胞遺傳學方法(CC)進行比較分析.結果 FISH法三體8的檢齣率較傳統吉姆薩顯帶高,或在吉姆薩顯帶無法分析時提供結果.結論 FISH檢測三體8的敏感性高于常規覈型分析,在小剋隆檢測方麵有其優越性.
목적 탐토간기형광원위잡교(FISH)기술재검측혈액병염색체삼체8중적개치.방법 용형광소직접표기적8호염색체착사립탐침대77례혈액병환자진행간기FISH검측,병여상규세포유전학방법(CC)진행비교분석.결과 FISH법삼체8적검출솔교전통길모살현대고,혹재길모살현대무법분석시제공결과.결론 FISH검측삼체8적민감성고우상규핵형분석,재소극륭검측방면유기우월성.
Objective To explore the value of interphase fluorescence in situ hybridization(FISH) in the detection of trisomy 8 in patients with hematologic disorders. Methods Seventy-seven patients were vestigated by directly labeled centrome DNA probes specific for 8 chromosome. The results were compared with that of conventional cytogenetic (CC) analysis. Results The proportion of trisomy 8 of 77 cases of hematologic disorders detected by FISH is higher than by G-banding karyotyping and FISH could offer the result when conventional cytogenetic methods failed to diagnose. Conclusion Interphase FISH is more sensitive in the detection of trisomy 8 than CC, and FISH displays its superiority in the detection of small clone.
