CAJ | 학술논문

目的磁共振成像(MRI)活体观察内膜损伤后小鼠颈动脉管腔狭窄和管壁增厚的动态过程.方法金属丝损伤法建立小鼠颈动脉内膜损伤模型,术后不同的时间点行MRI扫描,动态观察损伤血管的表现,包括管腔内径和面积、管壁的厚度和面积.结果 MRI能够清晰地观察小鼠颈动脉内膜损伤后的变化以及血管周围软组织的表现.小鼠颈动脉内膜损伤前、损伤后1、5、10d和15d管腔内径分别为(0.57±0.07)、(0.41±0.19)、(0.44±0.10)、(0.43±0.10)mm和(0.47±0.11)mm.管腔面积分别为:(0.30±0.06)、(0.18±0.11)、(0.18±0.06)、(0.18±0.06)mm2和(0.22±0.07)mm2.术后第15天患侧血管壁厚度为(0.23±0.12)mm,管壁面积为(0.35±0.24)mm2.结论 MRI可以在活体观察小鼠颈动脉内膜损伤后管腔狭窄和管壁增厚的动态变化过程.
목적 자공진성상(MRI)활체관찰내막손상후소서경동맥관강협착화관벽증후적동태과정.방법 금속사손상법건립소서경동맥내막손상모형,술후불동적시간점행MRI소묘,동태관찰손상혈관적표현,포괄관강내경화면적、관벽적후도화면적.결과 MRI능구청석지관찰소서경동맥내막손상후적변화이급혈관주위연조직적표현.소서경동맥내막손상전、손상후1、5、10d화15d관강내경분별위(0.57±0.07)、(0.41±0.19)、(0.44±0.10)、(0.43±0.10)mm화(0.47±0.11)mm.관강면적분별위:(0.30±0.06)、(0.18±0.11)、(0.18±0.06)、(0.18±0.06)mm2화(0.22±0.07)mm2.술후제15천환측혈관벽후도위(0.23±0.12)mm,관벽면적위(0.35±0.24)mm2.결론 MRI가이재활체관찰소서경동맥내막손상후관강협착화관벽증후적동태변화과정.
Objective To dynamically observe stenosis and wall thickness of carotid artery with endothelium injury in mouse using 7T micro-MR imaging in vivo. Methods A mouse model of carotid artery intimal injury was established by removing endothelium with a flexible wire. The lumen diameter, lumen area, wall thickness and wall area of the injured arteries were observed, and serial MR scanning was performed in different time points after operation. Results The injured arteries and perivascular parenchyma were clearly observed by MR imaging. Before and 1, 5, 10 and 15 days after artery injury, the lumen diameter were (0.57±0.07)mm,(0.41±0.19)mm, (0.44±0.10)mm, (0.43±0.10)mm and (0.47±0.11)mm respectively, and the lumen area were (0.30±0.06)mm2, (0.18±0.11)mm2, (0.18±0.06)mm2, (0.18±0.06)mm2 and (0.22±0.07)mm2. The thickness of artery wall was(0.23±0.12)mm, and the area of artery wall was (0.35±0.24)mm2 15days after artery injury. Conclusions Stenosis and wall thickening of carotid artery after the artery intimal injury of mouse can be dynamically observed on MR imaging in vivo.