CAJ | 학술논문

目的探讨多次床旁X线摄片对早产鼠肺组织氧自由基的影响.方法取孕21 dSD大鼠(因频繁运动出现早产)分娩的早产鼠为研究对象,按临床早产儿可能接受的X线次数分组进行照片,每天1次,同时设对照组,分别于第2、4、6、8、10次摄片后4h随机取实验组10只,对照组5～6只断头取肺组织,行形态学检查、脂质过氧化产物丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性的测定.结果连续10次(每天1次)摄片,对早产鼠体重、肺重以及肺的形态学无明显影响(P>0.05);MDA含量在第8次摄片后达到最高[(24.45±8.30)nmol/mg肺组织,SOD活性持续升高至本实验结束,最终达(16.26±3.84)nU/mg肺组织],和对照组相比,6次以上摄片实验组肺MDA和SOD含量高于对照组,差异有统计学意义(P<0.05).结论连续摄片具有剂量累加效应,超过6次床旁X线摄片的电离辐射能使早产子鼠体内氧化和抗氧化系统处于亢奋状态.
목적 탐토다차상방X선섭편대조산서폐조직양자유기적영향.방법 취잉21 dSD대서(인빈번운동출현조산)분면적조산서위연구대상,안림상조산인가능접수적X선차수분조진행조편,매천1차,동시설대조조,분별우제2、4、6、8、10차섭편후4h수궤취실험조10지,대조조5～6지단두취폐조직,행형태학검사、지질과양화산물병이철(MDA)함량화초양화물기화매(SOD)활성적측정.결과 련속10차(매천1차)섭편,대조산서체중、폐중이급폐적형태학무명현영향(P>0.05);MDA함량재제8차섭편후체도최고[(24.45±8.30)nmol/mg폐조직,SOD활성지속승고지본실험결속,최종체(16.26±3.84)nU/mg폐조직],화대조조상비,6차이상섭편실험조폐MDA화SOD함량고우대조조,차이유통계학의의(P<0.05).결론 련속섭편구유제량루가효응,초과6차상방X선섭편적전리복사능사조산자서체내양화화항양화계통처우항강상태.
Objective To investigate the effect of multiple bedside radiation on oxygen free radical in neonatal lung tissue.Methods SD rats,on the 21st day of pregnancy,were induced to preterm delivery by frequent movement.And the premature pups were randomly divided to experiment group,who received clinical X-ray once a day,and control group without.Four hours after receiving radiography for 2,4,6,8 and 10 times,10 rats from the experimental group and 5 to 6 rats from the control group were sacrificed.The morphological examination was performed,malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) were detected in the lung tissue.Results The consecutive radiography did not affect the lung weight,body weight and lung morphology of premature rats.MDA peaked to (24.45 ±8.30) nmol/mg after the 8th radiography,the activity of SOD increased to (16.26±3.84) nU/mg at the end of the experiment.Compared with the control group,more than six times radiography significantly increased MDA and SOD level (P<0.05).Conelusions Consecutive radiography may have dose cumulative effects on premature SD rats.More than six times bedside X-ray could stimulate the oxidation and antioxidant system of the body.