中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2011年
5期
413-416
,共4页
周昭彦%秦亮%胡必杰%渡边浩
週昭彥%秦亮%鬍必傑%渡邊浩
주소언%진량%호필걸%도변호
嗜肺军团菌%生物膜%毒力基因%RNA表达
嗜肺軍糰菌%生物膜%毒力基因%RNA錶達
기폐군단균%생물막%독력기인%RNA표체
LegioneUa pneumophilla%Biofilm%Violence gene%RNA expression
目的 初步探讨生物膜相关军团菌独特的毒力基因表达特征.方法 在富营养条件下建立静止状态的单细胞嗜肺军团菌生物膜模型.运用反转录real-time PCR技术,比较和分析浮游和生物膜状态下嗜肺军团菌部分毒力基因的表达,毒力基因包括mip、flaA和fliA基因.结果 对数生长后期与对数生长期浮游菌的mip、flaA和fliA基因表达量比值分别为0.53、4.45、3.67,对数生长期浮游菌显示复制态军团菌的毒力基凶表达特征,对数生长后期表达转移态军团菌特点.生物膜相关军团菌与对数生长期浮游菌的mip、flaA和fliA基因表达量比值为4.42、5.24、16.21;与对数生长后期浮游菌的比值为8.39、1.18、4.43,生物膜相关军团菌同时表达复制态和转移态军团菌毒力基因特征.结论 生物膜相关军团菌毒力基因的表达具有其特殊性,有待进一步研究.
目的 初步探討生物膜相關軍糰菌獨特的毒力基因錶達特徵.方法 在富營養條件下建立靜止狀態的單細胞嗜肺軍糰菌生物膜模型.運用反轉錄real-time PCR技術,比較和分析浮遊和生物膜狀態下嗜肺軍糰菌部分毒力基因的錶達,毒力基因包括mip、flaA和fliA基因.結果 對數生長後期與對數生長期浮遊菌的mip、flaA和fliA基因錶達量比值分彆為0.53、4.45、3.67,對數生長期浮遊菌顯示複製態軍糰菌的毒力基兇錶達特徵,對數生長後期錶達轉移態軍糰菌特點.生物膜相關軍糰菌與對數生長期浮遊菌的mip、flaA和fliA基因錶達量比值為4.42、5.24、16.21;與對數生長後期浮遊菌的比值為8.39、1.18、4.43,生物膜相關軍糰菌同時錶達複製態和轉移態軍糰菌毒力基因特徵.結論 生物膜相關軍糰菌毒力基因的錶達具有其特殊性,有待進一步研究.
목적 초보탐토생물막상관군단균독특적독력기인표체특정.방법 재부영양조건하건립정지상태적단세포기폐군단균생물막모형.운용반전록real-time PCR기술,비교화분석부유화생물막상태하기폐군단균부분독력기인적표체,독력기인포괄mip、flaA화fliA기인.결과 대수생장후기여대수생장기부유균적mip、flaA화fliA기인표체량비치분별위0.53、4.45、3.67,대수생장기부유균현시복제태군단균적독력기흉표체특정,대수생장후기표체전이태군단균특점.생물막상관군단균여대수생장기부유균적mip、flaA화fliA기인표체량비치위4.42、5.24、16.21;여대수생장후기부유균적비치위8.39、1.18、4.43,생물막상관군단균동시표체복제태화전이태군단균독력기인특정.결론 생물막상관군단균독력기인적표체구유기특수성,유대진일보연구.
Objective To investigate the physiological state of L. pneumophila in biofilm. Methods Genes previously identified as good markers for the transmissive and replicative phases of the L. pneumophila life cycle during growth in Acanthamoeba castellanii were examined for their expression fold change in the sessile cells as compared to planktonic cells using real-time RT PCR. Results Mature L. pneumophila biofilms were formed at 37t in 75 cm2 cell culture treated flasks for 18 days. The ratio of gene (mip, flaA and fliA) expression in post-exponential cells compared to exponential cells is 0. 53, 4. 45 and 3. 67. The exponential phase cultures display replicative traits and post-exponential bacteria express transmissive factors. The ratio of gene (mip, flaA and fliA) expression in sessile cells compared to exponential cells is 4.42, 5.24 and 16.21, while the sessile cells compared to exponential cells is 8.39, 1. 18 and 4. 43, respectively. Conclusion The violence gene expression of L pneumophila in biofilm is unique.
