中华整形外科杂志
中華整形外科雜誌
중화정형외과잡지
CHINESE JOURNAL OF PLASTIC SURGERY
2009年
4期
273-276
,共4页
陈刚%刘延山%刘毅%马恒香%王之奇%李宏捷
陳剛%劉延山%劉毅%馬恆香%王之奇%李宏捷
진강%류연산%류의%마항향%왕지기%리굉첩
骨生成,牵张%腭裂%胰岛素样生长因子-1%碱性磷酸酶
骨生成,牽張%腭裂%胰島素樣生長因子-1%堿性燐痠酶
골생성,견장%악렬%이도소양생장인자-1%감성린산매
Osteogenesis,distraction%Cleft palate%Insulin-like growth factor-1%Alkalinephosphatase
目的 以牵张成骨术整复猕猴腭裂骨缺损,定量分析不同时段新生成骨胰岛素样生长因子-1(insulin.1ike growth factor-I,IGF-1)与碱性磷酸酶(alkaline phosphatase,ALP)表达水平,探讨其成骨调控机制.方法 用猕猴建立腭裂动物模型.实验组动物21只以牵张成骨术整复其腭部软硬组织缺损,关闭裂隙后固定.于牵张成骨术后第1、2,4…6 8 12及24周分别取材,各3只动物.采用实时定量PCR法分析比较IGF-I与ALP的mRNA表达水平,并以酶联免疫吸附试验法(ELISA)定量分析其IGF-I与ALP含量,结果与实验对照及健康对照组(动物各2只)进行比较.结果 固定期第1～2周为成骨早期,第1周时IGF-1和AIJP的mRNA表达明显上调,分别为3.67±0.35和3.30±0.21,第2周时达最高峰,分别为7.55±0.32和5.91±0.21,随后逐渐下降,至第12周与健康对照组无明显差异(P>0.05).ELISA结果显示:固定期第1～2周,IGF-1和ALP表达均增强.IGF-1含量于第2周表达最高,为(2.0±0.06)ng/mg,第4周为(1.46±0.08)ns/mg,第6周为(0.84±0.11)ng/mg,其表达逐渐下降;同期ALP则呈高水平表达,第4周为(25.34±0.44)U/mg,第6周为(26.21±0.82)U/mg.第8～12周,IGF-1和ALP的表达均下降至接近健康对照组.结论 腭骨牵张成骨区域,新骨的原位增量生成明确,增殖过程正常,最终以膜内成骨的方式形成新骨整复了腭裂骨切开牵张间隙区域.
目的 以牽張成骨術整複獼猴腭裂骨缺損,定量分析不同時段新生成骨胰島素樣生長因子-1(insulin.1ike growth factor-I,IGF-1)與堿性燐痠酶(alkaline phosphatase,ALP)錶達水平,探討其成骨調控機製.方法 用獼猴建立腭裂動物模型.實驗組動物21隻以牽張成骨術整複其腭部軟硬組織缺損,關閉裂隙後固定.于牽張成骨術後第1、2,4…6 8 12及24週分彆取材,各3隻動物.採用實時定量PCR法分析比較IGF-I與ALP的mRNA錶達水平,併以酶聯免疫吸附試驗法(ELISA)定量分析其IGF-I與ALP含量,結果與實驗對照及健康對照組(動物各2隻)進行比較.結果 固定期第1～2週為成骨早期,第1週時IGF-1和AIJP的mRNA錶達明顯上調,分彆為3.67±0.35和3.30±0.21,第2週時達最高峰,分彆為7.55±0.32和5.91±0.21,隨後逐漸下降,至第12週與健康對照組無明顯差異(P>0.05).ELISA結果顯示:固定期第1～2週,IGF-1和ALP錶達均增彊.IGF-1含量于第2週錶達最高,為(2.0±0.06)ng/mg,第4週為(1.46±0.08)ns/mg,第6週為(0.84±0.11)ng/mg,其錶達逐漸下降;同期ALP則呈高水平錶達,第4週為(25.34±0.44)U/mg,第6週為(26.21±0.82)U/mg.第8～12週,IGF-1和ALP的錶達均下降至接近健康對照組.結論 腭骨牽張成骨區域,新骨的原位增量生成明確,增殖過程正常,最終以膜內成骨的方式形成新骨整複瞭腭裂骨切開牽張間隙區域.
목적 이견장성골술정복미후악렬골결손,정량분석불동시단신생성골이도소양생장인자-1(insulin.1ike growth factor-I,IGF-1)여감성린산매(alkaline phosphatase,ALP)표체수평,탐토기성골조공궤제.방법 용미후건립악렬동물모형.실험조동물21지이견장성골술정복기악부연경조직결손,관폐렬극후고정.우견장성골술후제1、2,4…6 8 12급24주분별취재,각3지동물.채용실시정량PCR법분석비교IGF-I여ALP적mRNA표체수평,병이매련면역흡부시험법(ELISA)정량분석기IGF-I여ALP함량,결과여실험대조급건강대조조(동물각2지)진행비교.결과 고정기제1～2주위성골조기,제1주시IGF-1화AIJP적mRNA표체명현상조,분별위3.67±0.35화3.30±0.21,제2주시체최고봉,분별위7.55±0.32화5.91±0.21,수후축점하강,지제12주여건강대조조무명현차이(P>0.05).ELISA결과현시:고정기제1～2주,IGF-1화ALP표체균증강.IGF-1함량우제2주표체최고,위(2.0±0.06)ng/mg,제4주위(1.46±0.08)ns/mg,제6주위(0.84±0.11)ng/mg,기표체축점하강;동기ALP칙정고수평표체,제4주위(25.34±0.44)U/mg,제6주위(26.21±0.82)U/mg.제8～12주,IGF-1화ALP적표체균하강지접근건강대조조.결론 악골견장성골구역,신골적원위증량생성명학,증식과정정상,최종이막내성골적방식형성신골정복료악렬골절개견장간극구역.
Objective To investigate the osteogenesis mechanism by analysis of the expression of insulin-like growth factor-I (IGF-1)and alkaline phesphatas (ALP)in the reconstruction of cleft palate(CP) with distraction osteogenesis (DO) in rhesus. Methods The CP animal models were established surgically. 21 rhesus in experimental group underwent DO to close the soft and bony defect, followed by consolidations. Every 3 animals were killed and the specimen were taken out after consolidation of 1, 2, 4, 6, 8, 12, 24 weeks. The mRNA of IGF-1 and ALP were detected with Real-time BT-PCB technique. The expression of IGF-1 and ALP was quantitatively analyzed by ELISA. The results were compared with those in control and sham groups (each of 2 animals), respectively. Results Since consolidation, the mRNA of IGF-1 and ALP increased significantly at one week and reached the peak at two weeks, but decrease to control level after 12 weeks of consolidation. The expression of IGF-1 also increased to peak level afiert two weeks of consolidation. The expression of ALT increased significantly since consolidation and reach the peak value after six weeks. They all decreased to nearly control level after 8 ～ 12 weeks. Conclusions The palate cleft can be successfully closed with new formed bone after DO. The mechanism of bone consolidation is intramembranons bone formation.
