CAJ | 학술논문

目的　评价聚合酶链反应（PCR ）分子灯塔法检测临床标本中结核分枝杆菌（结核菌）的应用价值。方法　应用涂片镜检法、培养法及PCR分子灯塔法检测142份结核病患者痰标本，42份非结核呼吸系疾病患者痰标本。结果　涂片镜检法检测结核病患者临床标本中结核菌阳性率为35.2%(50/142)，培养法阳性率为35.9%(51/142)， PCR分子灯塔法阳性率为44.4%（63/142）。用PCR分子灯塔法检测临床标本特异性为100%。结论　PCR 分子灯塔法是一种全新的PCR分子杂交模式，PCR扩增、荧光探针杂交、检测一体化，在单一管内完成，其简便、快速、防污染，敏感性较高、特异性强，是结核病辅助诊断的有效方法之一。
목적　평개취합매련반응（PCR ）분자등탑법검측림상표본중결핵분지간균（결핵균）적응용개치。방법　응용도편경검법、배양법급PCR분자등탑법검측142빈결핵병환자담표본，42빈비결핵호흡계질병환자담표본。결과　도편경검법검측결핵병환자림상표본중결핵균양성솔위35.2%(50/142)，배양법양성솔위35.9%(51/142)， PCR분자등탑법양성솔위44.4%（63/142）。용PCR분자등탑법검측림상표본특이성위100%。결론　PCR 분자등탑법시일충전신적PCR분자잡교모식，PCR확증、형광탐침잡교、검측일체화，재단일관내완성，기간편、쾌속、방오염，민감성교고、특이성강，시결핵병보조진단적유효방법지일。
Objective　To evaluate the value of PCR-molecular beacon assay. In detecting mycobacterium tuberculosis in clinical specimens. Methods　The sputum specimens of 142 patients with pulmonary tuberculosis and 42 patients with respiratory system diseases other than tuberculosis were tested by smears, cultures and PCR-molecular beacon assays. Results　The sensitivities of smears. cultures and molecular beacon assay in detecting mycobacterium tuberculosis in sputum specimens of patients with pulmonary tuberulosis were 35.2%,35.9% and 44. 4%, respectively. The specificity of molecular beacon assay in testing clinical specimens was 100%. Conclusion　 PCR-molecular beacon assay is in a hermetially sealed simple tube, rapid and anticontaminative. This assay is higher in sensitivity and specifity,than smears and cultures is one of the effective mothods for diagnosis of tuberculosis.