CAJ | 학술논문

目的研究脉冲强磁场对人膀胱肿瘤BIU-87细胞生长相关基因表达的影响.方法将体外培养的人膀胱肿瘤BIU-87细胞分为磁场组及对照组.磁场组细胞给予脉冲强磁场干预(磁场强度为8 T,频率为15 Hz),每天持续干预2 h;对照组细胞亦置于相同环境中,但未给予曝磁处理.分别于实验进行24 h、48 h及72 h后采用逆转录-多聚酶链反应(RT-PCR)技术检测各组细胞B细胞淋巴瘤/白血病基因-2(Bcl-2)、促凋亡基因(Bax)及凋亡促进因子-3(caspase-3)mRNA表达,采用流式细胞仪检测Bcl-2、Bax及caspase-3蛋白表达.结果实验进行24 h、48 h及72 h后,发现磁场组Bcl-2 mRNA及蛋白表达在上述各时间点均较对照组明显降低,Bax mRNA及蛋白表达均较对照组显著增强(P＜0.05);caspase-3 mRNA及蛋白表达在上述各时间点组间差异均无统计学意义(P＞0.05).结论脉冲强磁场能抑制膀胱肿瘤BIU-87细胞生长,促其凋亡,其机制可能与脉冲强磁场促进Bax mRNA及蛋白表达、抑制Bcl-2 mRNA及蛋白表达有关.
목적 연구맥충강자장대인방광종류BIU-87세포생장상관기인표체적영향.방법 장체외배양적인방광종류BIU-87세포분위자장조급대조조.자장조세포급여맥충강자장간예(자장강도위8 T,빈솔위15 Hz),매천지속간예2 h;대조조세포역치우상동배경중,단미급여폭자처리.분별우실험진행24 h、48 h급72 h후채용역전록-다취매련반응(RT-PCR)기술검측각조세포B세포림파류/백혈병기인-2(Bcl-2)、촉조망기인(Bax)급조망촉진인자-3(caspase-3)mRNA표체,채용류식세포의검측Bcl-2、Bax급caspase-3단백표체.결과 실험진행24 h、48 h급72 h후,발현자장조Bcl-2 mRNA급단백표체재상술각시간점균교대조조명현강저,Bax mRNA급단백표체균교대조조현저증강(P＜0.05);caspase-3 mRNA급단백표체재상술각시간점조간차이균무통계학의의(P＞0.05).결론 맥충강자장능억제방광종류BIU-87세포생장,촉기조망,기궤제가능여맥충강자장촉진Bax mRNA급단백표체、억제Bcl-2 mRNA급단백표체유관.
Objective To estimate any influence of strong pulsed magnetic fields on the expression of growth-related genes in human bladder cancer BIU-87 cells. Methods Human BIU-87 cells were cultured in vitro and randomly divided into a magnetic field group and a control group. Each group was further divided into 24 h, 48 h and 72 h sub-groups. The magnetic field group cells were exposed to an 8 T magnetic field pulsed at 15 Hz for 2 h every day. The control group cells also placed on the same environment, but not exposed to any strong, pulsed magnetic field. The expression of B cell lymphoma/leukemia gene-2 (Bcl-2) mRNA, Bax mRNA and caspase-3 mRNA was measured with RT-PCR, and flow cytometry was used to evaluate the expression of the Bcl-2, Bax and caspase-3 genes of the tumor cells in vitro. Results The expression of Bax mRNA and protein was significantly higher in the cells exposed to the magnetic field than in the control groups. The expression of Bcl-2 mRNA and protein was significantly less. The expression of caspase-3 mRNA and protein in the two groups showed no significant differences.Conclusions A strong, pulsed magnetic field can inhibit the growth of bladder tumor BIU-87 cells and promote their apoptosis. The mechanism is probably related with the magnetic field promoting Bax mRNA and protein expression and inhibiting Bcl-2 mRNA and protein expression.