CAJ | 학술논문

目的观察超短波及低频脉冲磁疗对骨折愈合过程中血管内皮生长因子(VEGF)表达的影响.方法选用新西兰大白兔56只,随机分为对照组、超短波治疗组(超短波组)、低频脉冲磁疗组(磁疗组)和超短波+低频脉冲磁疗组(联合组),每组14只.各组分别制备桡骨横断骨折模型.对照组不予干预,其余各组分别给予超短波及低频脉冲磁疗治疗.分别于术后第1,2,4,6周拍摄X线片,评价骨痂以及骨折愈合情况;于术后第1,2,4,6周取材行病理学检查,观察骨折愈合情况,并行免疫组织化学染色检测VEGF蛋白表达水平.结果 X线片显示,术后第1、2、4、6周,联合组骨痂生成增多,骨折线模糊,X线评分大于其他组,差异具有统计学意义(P<0.05).病理检查结果示,与对照组、超短波组及磁疗组相比,联合组在术后各相应时间点软骨岛生成多,骨痂生成多,重建快,骨折愈合时间短.免疫组织化学染色检测结果示,联合组第1、2、4周的VEGF免疫阳性指数大于对照组、超短波组及磁疗组,差异有统计学意义(P<0.05).结论超短波结合低频脉冲磁疗可上调骨折愈合过程中VEGF的表达,从而促进骨痂生长,加快骨折愈合.
목적 관찰초단파급저빈맥충자료대골절유합과정중혈관내피생장인자(VEGF)표체적영향.방법 선용신서란대백토56지,수궤분위대조조、초단파치료조(초단파조)、저빈맥충자료조(자료조)화초단파+저빈맥충자료조(연합조),매조14지.각조분별제비뇨골횡단골절모형.대조조불여간예,기여각조분별급여초단파급저빈맥충자료치료.분별우술후제1,2,4,6주박섭X선편,평개골가이급골절유합정황;우술후제1,2,4,6주취재행병이학검사,관찰골절유합정황,병행면역조직화학염색검측VEGF단백표체수평.결과 X선편현시,술후제1、2、4、6주,연합조골가생성증다,골절선모호,X선평분대우기타조,차이구유통계학의의(P<0.05).병리검사결과시,여대조조、초단파조급자료조상비,연합조재술후각상응시간점연골도생성다,골가생성다,중건쾌,골절유합시간단.면역조직화학염색검측결과시,연합조제1、2、4주적VEGF면역양성지수대우대조조、초단파조급자료조,차이유통계학의의(P<0.05).결론 초단파결합저빈맥충자료가상조골절유합과정중VEGF적표체,종이촉진골가생장,가쾌골절유합.
Objective To study the effects of uhrashortwave and low frequency pulsed electromagnetic fields on the expression of vascular endothelial growth factor(VEGF) in fracture healing. Methods Fifty-six New Zeal-and rabbits with artificial fractures were randomly divided into 4 groups:a control group,an ultrashortwave group,a low frequency pulsed electromagnetic field group and an ultrashortwave combined with low frequency pulsed electro-magnetic field group(combined group),with 14 in each group.Radiographic evaluation of callus formation and frac-ture healing,pathohistological examination and detection of VEGF expression through immunohistochemical staining were performed at the 1 st,2nd,4th and 6th week after the operation. Results Radiographic examination showed that there was significantly greater callus formation in the combined group than in the other groups throughout the healing process. Pathohistological examination also revealed significantly more cartilage islets and callus formation in the combined group.At the 1 st,2nd and 4th week after the operation,VEGF positive indexes in the combined group were significantly higher than in the other groups. Conclusion Uhrashortwave combined with low frequency pulsed electromagnetic field exposure can up-regulate the expression of VEGF and thus can accelerate fracture healing.